Sugar uptake and invertase activity in <i>Dendrobium</i> tissues

Hew, C. S.; Mah, T. C.

doi:10.1111/j.1469-8137.1989.tb00678.x

Cited by 12 publications

(6 citation statements)

References 20 publications

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“…Hew and Mah (1989) reported that the percentage increase in dry matter of differentiated Dendrobium Multico White tissues grown in vitro was higher at 2% than at 1% sucrose. Vaz (2002), on the other hand, detected a significant decrease in the dry matter of Psygmorchis pusilla shoots grown in vitro in sucrose concentrations up to 6%.…”

Section: Resultsmentioning

confidence: 97%

Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark

Ferreira

Suzuki

Pescador

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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In general, plant material grown in vitro has low photosynthetic ability to achieve positive carbon balances. Therefore, a continuous supply of carbohydrates from the culture medium is required, and sucrose has been the most commonly used carbon source. In this paper, we investigate the effects of different sucrose concentrations and the presence and absence of light on the endogenous levels of soluble carbohydrates and starch as well as on the proliferation and growth of Dendrobium Second Love (Orchidaceae) in vitro. The possibility of using etiolated stem segments as a means for micropropagating this hybrid was also verified. The results obtained indicated that the presence and absence of light and the sucrose concentrations used influenced the amounts of soluble carbohydrates and starch and the proliferation of D. Second Love shoots and roots. An increase in sucrose concentration caused a progressive increase in the amounts of total carbohydrates and starch. Under both light conditions, sucrose was the main sugar found in the shoots followed by glucose and fructose. The addition of sucrose to the culture medium up to 2% and 4% was advantageous to the number of shoots produced per explant and the root longitudinal growth in the presence and absence of light, respectively. Shoot and root dry matter and the number of roots formed per explant increased as sucrose concentration was raised up to 6% in both light treatments. The use of dark-grown shoot segments proved to be a useful and reliable alternative for the micropropagation of this hybrid.

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Section: Resultsmentioning

confidence: 97%

Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark

Ferreira

Suzuki

Pescador

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…3). Contradictory results on the effect of autoclaved fructose as a carbon source (Oka & Ohyama 1986;Chauvin & Salesses 1988;Hew & Mah 1989;Welander et al 1989;Borkowska & Szczerba 1991;Moncousin et al 1992) are probably due to differences in the sensitivity of plant species to the degradation products formed as a result of autoclaving, such as furfural and hydroxymethylfurfural (Hsiao & Bornman 1989;Uosukainen & Vasara 1992;Druart & de Wulf 1993).…”

Section: Discussionmentioning

confidence: 99%

Role of carbohydrates in micropropagation of cork oak

Romano

Noronha

Martins-Loução

1995

Plant Cell Tiss Organ Cult

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The influences of carbon sources, fructose, glucose, sorbitol and sucrose on shoot proliferation and in vitro rooting of cork oak (Quercus suber L.) were compared at a wide range of concentrations (1-6%, w/v). The highest number of shoots occurred on glucose-containing medium. Nevertheless, we have chosen 3% sucrose which induced a similar rate of proliferation but favoured shoot elongation, permitting an effectively higher number of shoots during transfers. Sorbitol and autoclaved fructose did not stimulate shoot proliferation. Adventitious root formation was strongly dependent on carbohydrate supply. Sorbitol and autoclaved fructose were completely ineffectively on rooting induction. Glucose was the most effective carbon source on rooting promotion followed by sucrose and filter-sterilized fructose. The rooting response induced by fructose was dependent on the sterilizing procedure. The number of adventitious roots produced per shoot increased with increasing glucose and sucrose concentration. The content of reducing sugars in leaves of proliferation cultures and in leaves and roots of rooted plantlets was more dependent on carbon concentration than on glucose or sucrose supplement. The results presented here show that carbohydrate requirements during cork oak micropropagation depend upon the phase of culture. Sucrose (3%) and glucose (4%) were the best carbon sources respectively during proliferation and rooting phases.

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“…A preferência pela frutose em relação à sacarose, como fonte de carbono, por algumas espécies e tecidos, pode estar relacionada à necessidade de as células excretarem a enzima invertase ao meio de cultivo, visando à hidrólise da sacarose antes da absorção da fonte de carbono (Hew & Mah, 1989). No presente experimento com P. glomerata, verificou-se que a sacarose foi a melhor fonte de carbono para o crescimento; portanto, essa espécie não apresenta dificuldades em absorver a sacarose diretamente do meio e/ou também seus produtos de hidrólise.…”

Section: (D)unclassified

Efeito de doses e fontes de carboidratos no crescimento de plantas de ginseng Brasileiro [Pfaffia glomerata (Spreng.) Pedersen] Cultivadas in vitro

et al. 2003

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RESUMOPfaffia glomerata (Spreng.) Pedersen é uma das espécies de ginseng brasileiro usada popularmente como planta medicinal. Com este trabalho objetivou-se avaliar o efeito de doses (15, 30, 45 e 60 g.L -1 ) e fontes de carboidratos (sacarose, frutose, glicose, maltose e lactose) no crescimento dessas plantas cultivadas in vitro. O delineamento experimental foi o inteiramente casualizado com 15 repetições por tratamento. A parcela experimental consistiu de um tubo de ensaio contendo 10 mL de meio MS e um segmento nodal de 1,0 cm de comprimento e sem folhas. Os parâmetros avaliados aos 41 dias após a inoculação foram o número de brotações, o número total de segmentos nodais por plântula, a altura média das brotações, a altura da maior brotação, a massa fresca e seca da parte aérea e a massa seca das raízes. A melhor fonte de carbono para o crescimento in vitro da P. glomerata foi a sacarose, e a lactose é inapropriada. O número total de segmentos nodais, altura média das brotações, altura da maior brotação, massa fresca e seca da parte aérea e a massa seca de raízes aumentam pelo acréscimo das doses de sacarose, e em 30, 45 e 60 g.L -1 foram significativamente maiores do que aquelas proporcionadas pelas demais fontes de carbono. TERMOS PARA INDEXAÇÃO:Cultura de tecidos, espécie medicinal, açúcares, Amaranthaceae. EFFECT OF CARBOHYDRATE CONCENTRATION AND SOURCE ON GROWTH OF BRAZILIAN GINSENG [Pfaffia glomerata (Spreng.) Pedersen] IN VITRO CULTURED PLANTSABSTRACT Pfaffia glomerata (Spreng.) Pedersen is one of the brazilian ginseng species used as medicinal plant. The aim of this work was to evaluate the effect of carbohydrate concentration (15, 30, 45, and 60 g.L -1 ) and source (sucrose, fructose, glucose, maltose, and lactose) on the growth of plants grown in vitro. An entirely random statistical design with 15 replications per treatment was used. The experimental unity consisted of a test tube containing 10 mL of MS medium and 1-node segment of 1,0cm length and without leaves. After 41 days of the inoculation the growth parameters analyzed were sprout number, total number of nodal segment per seedling, average length of sprouts, length of the highest sprout, fresh and dry matter weight of aerial parts, and root dry matter weight. The best carbohydrate source for growth of P. glomerata in vitro cultured plants was sucrose, and lactose was found unsuitable. The total number of nodal segment per seedling, average length of sprouts, length of the highest sprout, fresh and dry matter weight of aerial parts, and root dry matter weight increase by sucrose concentrations, and at 30, 45 and 60 g.L -1 all parameters were significantly higher than those obtained by other carbon sources.

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Sugar uptake and invertase activity in Dendrobium tissues

Cited by 12 publications

References 20 publications

Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark

Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark

Role of carbohydrates in micropropagation of cork oak

Efeito de doses e fontes de carboidratos no crescimento de plantas de ginseng Brasileiro [Pfaffia glomerata (Spreng.) Pedersen] Cultivadas in vitro

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