Sugarcane Bagasse Pretreatment Methods for Ethanol Production

Saleh, Sabiha Hanim; Halim, Nurul Asyikin Abd

doi:10.5772/intechopen.81656

Cited by 19 publications

(12 citation statements)

References 92 publications

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“…Dilute acid (DA) and hydrothermal (HT) pretreatments are two examples of well-established pretreatments commonly used for sugarcane biomass [12–15]. They both create an acidic environment which loosens the cell wall by targeting the removal of xylan and are favored over other pretreatments because they are inexpensive and efficient in hydrolyzing lignocellulose [16]. Pretreatment using ionic liquids (IL) is emerging as a promising method because a large proportion of the lignin present in the biomass is solubilized allowing for high sugar yields from saccharification [17].…”

Section: Introductionmentioning

confidence: 99%

Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

Hodgson‐Kratky

Papa

Rodríguez

et al. 2019

Biotechnol Biofuels

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Background Lignocellulosic biomass is recognized as a promising renewable feedstock for the production of biofuels. However, current methods for converting biomass into fermentable sugars are considered too expensive and inefficient due to the recalcitrance of the secondary cell wall. Biomass composition can be modified to create varieties that are efficiently broken down to release cell wall sugars. This study focused on identifying the key biomass components influencing plant cell wall recalcitrance that can be targeted for selection in sugarcane, an important and abundant source of biomass. Results Biomass composition and the amount of glucan converted into glucose after saccharification were measured in leaf and culm tissues from seven sugarcane genotypes varying in fiber composition after no pretreatment and dilute acid, hydrothermal and ionic liquid pretreatments. In extractives-free sugarcane leaf and culm tissue, glucan, xylan, acid-insoluble lignin (AIL) and acid-soluble lignin (ASL) ranged from 20 to 32%, 15% to 21%, 14% to 20% and 2% to 4%, respectively. The ratio of syringyl (S) to guaiacyl (G) content in the lignin ranged from 1.5 to 2.2 in the culm and from 0.65 to 1.1 in the leaf. Hydrothermal and dilute acid pretreatments predominantly reduced xylan content, while the ionic liquid (IL) pretreatment targeted AIL reduction. The amount of glucan converted into glucose after 26 h of pre-saccharification was highest after IL pretreatment (42% in culm and 63.5% in leaf) compared to the other pretreatments. Additionally, glucan conversion in leaf tissues was approximately 1.5-fold of that in culm tissues. Percent glucan conversion varied between genotypes but there was no genotype that was superior to all others across the pretreatment groups. Path analysis revealed that S/G ratio, AIL and xylan had the strongest negative associations with percent glucan conversion, while ASL and glucan content had strong positive influences. Conclusion To improve saccharification efficiency of lignocellulosic biomass, breeders should focus on reducing S/G ratio, xylan and AIL content and increasing ASL and glucan content. This will be key for the development of sugarcane varieties for bioenergy uses.

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Section: Introductionmentioning

confidence: 99%

Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

Hodgson‐Kratky

Papa

Rodríguez

et al. 2019

Biotechnol Biofuels

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“…A typical bioethanol conversion relies on enzymes for converting biomass to ethanol and is done through this series of steps: food waste selection, pretreatment, saccharification and fermentation of the resulting sugars into ethanol, and purification (Rastogi & Shrivastava, 2017;Sabiha-Hanim & Halim, 2019). As seen in Figure 1, the schematic diagram represents the cradle-to-gate bioethanol production process.…”

Section: Bioethanol Productionmentioning

confidence: 99%

A Review on Bioethanol Production through the Valorization of Food Waste in Indonesia

Trisna¹,

Jai²,

Shirleen³

et al. 2022

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Biofuels are one of the numerous alternatives that are currently being considered to replace fossil fuels as it is more environmentally friendly. Specifically, bioethanol is often thought as a better alternative to gasoline fuel as it is considered cleaner, more renewable, and greener as it is synthesized from renewable feedstock which contributes to the reduction of greenhouse gas emissions to the environment. As bioethanol is produced from carbohydrate and starch rich food crops, food waste (FW) poses a potential source for bioethanol production as it is especially rich in carbohydrates and lipids. Bioethanol production itself consists of several steps which includes food waste selection, pretreatment, saccharification and fermentation, and recovery. Cafeteria FW was reviewed to be the best type of FW for bioethanol production as it has the highest carbohydrate and starch content. Subsequently, acid pretreatment was considered to be the best method due to low cost, high yielding, and time efficient method. Moreover, the non-isothermal simultaneous saccharification and fermentation (NSSF) produces 1.42 g ethanol/L.h with a time of 38 hours. Lastly, the enzyme-assisted extraction technique is most preferred to recover the bioactive compounds as it led to the highest yield of product (94%) compared to other methods.

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“…The pretreatments are necessary for efficient enzymatic hydrolysis of cellulose by removal of lignin and hemicelluloses [45]. Pretreatments also help to disrupt the crystalline structure of cellulose for enhancing enzyme accessibility to the cellulose during the enzymatic hydrolysis step [45]. The lignocellulosic composition of flaxseed meal after different pretreatment was very important which controlled the efficiency of enzymatic hydrolysis.…”

Section: Pretreatments Of Flaxseed Mealmentioning

confidence: 99%

Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

Ghosal¹,

Bhowal²

2021

Preprint

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The present study investigated the usefulness of flaxseed meals as a novel feedstock for the production of bioethanol. The proximate composition of the flaxseed meal was carried out before the pretreatment of the flaxseed meal. In this study, flaxseed meal was pretreated with dilute acid, alkali, and aqueous for disruption of lignocellulosic compounds. The acid pretreated flaxseed meal was used for enzymatic hydrolysis by different enzymes (cellulase, α-amylase, and cellulase combined with α-amylase) for better release of reducing sugar. The cellulose conversion to reducing sugar was significantly higher for acid pretreated flaxseed meals. After enzymatic hydrolysis with cellulase, cellulose conversions to reducing sugars were found to be significantly higher than those of α-amylase and cellulase combined with α-amylase. The bioethanol production was also investigated. The fermentation process was carried out by using baker’s yeast (Saccharomyces cerevisiae) with the acid pretreated flaxseed meal enzymatic hydrolyzate. Maximum ethanol production (0.11 g/l) was achieved from the fermented medium obtained from the acid pretreated flaxseed meal followed by enzymatic hydrolysis by using cellulase enzyme. The structural analysis of bioethanol was also investigated by FTIR.

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Sugarcane Bagasse Pretreatment Methods for Ethanol Production

Cited by 19 publications

References 92 publications

Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

A Review on Bioethanol Production through the Valorization of Food Waste in Indonesia

Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

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