Sugarcane Internode Composition During Crop Development

Lingle, Sarah E.; Thomson, Jessica L.

doi:10.1007/s12155-011-9153-3

Cited by 36 publications

(42 citation statements)

References 26 publications

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“…Cellulose, hemicellulose, and lignin are critical factors affecting forage digestibility and livestock industry. In agreement with the previous studies (Chen et al, 2002; Jung and Casler, 2006; Lingle and Thomson, 2012; Rancour et al, 2012; Zhang et al, 2013), a continuous increase in lignin accumulation was observed in internode 2 from the E2 to E4 stage and the successive ones along the culm at the R1 stage as well. Moreover, an impressive shifting of lignin composition was observed in internodes at late stages in sheepgrass, implying that S lignin units may play an important role in cell wall strengthen and mechanical support of culms.…”

Section: Discussionsupporting

confidence: 92%

Lignification of Sheepgrass Internodes at Different Developmental Stages and Associated Alteration of Cell Wall Saccharification Efficiency

Wang¹,

Shen³

et al. 2017

Front. Plant Sci.

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Sheepgrass (Leymus chinensis) is a high-quality cool-season forage crop used as pasture and hay for livestock feeds. The presence of lignin in cell walls, however, impairs forage digestibility of such lignocellulosic feedstock. Here, the structural characterization and cell wall composition of sheepgrass internodes were studied, and a progressive increase in cell wall lignification was observed with internode maturation. Lignin composition analysis further revealed a gradual accumulation of guaiacyl and syringyl lignin units during internode development. Consistently, the transcript abundance of lignin-related genes was upregulated in mature internodes, suggesting their potential roles in lignin biosynthesis. Furthermore, the effects of cell wall composition and lignification extent on biomass saccharification efficiency were examined in sheepgrass. The results showed that lignin content, guaiacyl and syringyl lignin unit levels inversely correlated with cell wall digestibility, indicating that lignin is a crucial obstacle for utilizing sheepgrass feedstock. The baseline information obtained in this work will facilitate establishment, grazing management, harvesting and feedstock utilization of sheepgrass in future.

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Section: Discussionsupporting

confidence: 92%

Lignification of Sheepgrass Internodes at Different Developmental Stages and Associated Alteration of Cell Wall Saccharification Efficiency

Wang¹,

Shen³

et al. 2017

Front. Plant Sci.

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“…The lignin content measured with the Klason method showed a continuous increase until the fifth internode for IACSP04-683 and until the sixth internode for IACSP04-529, after which much less variation was observed during later stages of stem development. This pattern of lignification is in agreement with previous reports (Lingle and Thomson, 2012). A similar developmental pattern of lignin deposition was observed in switchgrass (Panicum virgatum), a C4 perennial forage grass that has been selected for development as a biofuel crop in North America, and for which a fast increase in lignin content was observed during the progressive elongation stage (Shen et al, 2009).…”

Section: Discussionsupporting

confidence: 92%

Lignification in Sugarcane: Biochemical Characterization, Gene Discovery, and Expression Analysis in Two Genotypes Contrasting for Lignin Content

et al. 2013

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Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse into ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. Because several studies have shown a negative effect of lignin on saccharification yield, the characterization of lignin biosynthesis, structure, and deposition in sugarcane is an important goal. Here, we present, to our knowledge, the first systematic study of lignin deposition during sugarcane stem development, using histological, biochemical, and transcriptional data derived from two sugarcane genotypes with contrasting lignin contents. Lignin amount and composition were determined in rind (outer) and pith (inner) tissues throughout stem development. In addition, the phenolic metabolome was analyzed by ultra-highperformance liquid chromatography-mass spectrometry, which allowed the identification of 35 compounds related to the phenylpropanoid pathway and monolignol biosynthesis. Furthermore, the Sugarcane EST Database was extensively surveyed to identify lignin biosynthetic gene homologs, and the expression of all identified genes during stem development was determined by quantitative reverse transcription-polymerase chain reaction. Our data provide, to our knowledge, the first in-depth characterization of lignin biosynthesis in sugarcane and form the baseline for the rational metabolic engineering of sugarcane feedstock for bioenergy purposes.

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“…The results of the second experiment are similar to the maize results of Jung and Casler (2006), who suggest that young maize tissue is comprised of a higher percentage of lignified protoxylem vessels than more mature tissue, that initially results in a high lignin content in very young tissue (Jung & Casler 2006). It is likely that the Section A tissue (from internodes 2 and 3) was in what is the second zone identified in the studies by Jung and Casler (Jung & Casler 2006) and Lingle and Thomson (Lingle & Thomson 2012). This is supported by the results of Bottcher et al (2013) who showed lower lignin levels in internode 2-4 of two sugarcane cultivars before reaching a relatively steady state lignin level for internodes 5 to 18.…”

Section: Discussionsupporting

confidence: 81%

Peer Review #2 of "Cell wall composition and lignin biosynthetic gene expression along a developmental gradient in an Australian sugarcane cultivar (v0.1)"

Brito¹

2017

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Sugarcane Internode Composition During Crop Development

Cited by 36 publications

References 26 publications

Lignification of Sheepgrass Internodes at Different Developmental Stages and Associated Alteration of Cell Wall Saccharification Efficiency

Lignification of Sheepgrass Internodes at Different Developmental Stages and Associated Alteration of Cell Wall Saccharification Efficiency

Lignification in Sugarcane: Biochemical Characterization, Gene Discovery, and Expression Analysis in Two Genotypes Contrasting for Lignin Content

Peer Review #2 of "Cell wall composition and lignin biosynthetic gene expression along a developmental gradient in an Australian sugarcane cultivar (v0.1)"

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