Sugarcane metabolites produced in CO2-rich temporary immersion bioreactors (TIBs) induce tomato (Solanum lycopersicum) resistance against bacterial wilt (Ralstonia solanacearum)

Abstract

The production of phenolic metabolites has been optimized in parallel to sugarcane micropropagation in temporary immersion bioreactors (TIBs). Culturing micropropagated plants in 0.4% CO 2 -rich air induced their photosynthetic activity by enhancing the change from a heterotrophic to a photomixotrophic metabolic stage. Under 0.4% CO 2 enrichment, the transcript levels of both phenylalanine ammonia-lyase (PAL EF189195) and ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco CF576750) increased and were correlated to a 4.6-and 6.3-fold increase in the phenolic levels when plants were multiplied in 20 or 30 g/l sucrose, respectively. A novel application of plant phenolic metabolites as elicitors of resistance to tomato bacterial wilt in the Solanum lycopersicum-Ralstonia solanacearum pathosystem has been identified. The culture media was collected, and the phenolics were sprayed onto tomato plants infected with R. solanacearum, eliciting and/or maintaining an early defense signaling mechanism that resulted in the protection of the plant against the tomato bacterial wilt disease. RT-PCR analyses confirmed that selected genes from defense-related pathways were differentially expressed between plants treated with sugarcane metabolites, non-treated pathogenfree plants, and non-treated plants inoculated with R. solanacearum.

Temporary immersion systems in plant biotechnology

“…Twin‐Flask systems are also not equipped with a specialized port for external CO 2 supply during the exposure period. However, CO 2 ‐enriched air may be used to ensure higher CO 2 concentrations in the gaseous environment of the culture chamber . Twin‐Flask systems have been successfully applied in the propagation of plant seedlings, shoots, nodule cluster, and embryo cultures .…”

“…Twin‐Flask systems have been successfully applied in the propagation of plant seedlings, shoots, nodule cluster, and embryo cultures . Recently, Twin‐Flask systems have been used in research work on secondary metabolites accumulation by differentiated in vitro cultures as well .…”

“…Basically, the Twin-Flask system consists of two containers (wide-mouth flasks, bottles, or jars), connected together by a U pipe (glass or plastic) or a silicone tube ( Fig. 1) [16,17,[20][21][22][23][24]. One of the containers has the function of a culture chamber, whereas the other container is used as a medium storage tank.…”

EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee

“…(Etienne et al 2006). Penelitian penggunaan RITA ® juga dilaporkan lebih baik disbandingkan dengan media cair dan media semi padat pada tanaman Saccharum officinarum (Yang et al 2010) ; Eucalyptus Sp (McAlister et al 2005); Ananas comosus (pineapple) (Scheidt et al 2009). …”

Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile

“…RT-PCRs were conducted according to Yang et al (2010). RNA was extracted from the leaves (middle part) pooled from separate replicates.…”