Sugarcane Transformation

Snyman, S. J.

doi:10.1007/978-1-4020-2333-0_8

Cited by 17 publications

(3 citation statements)

References 42 publications

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“…Following the method described by Snyman (2004), sugarcane callus was induced from immature leaf rolls of the Saccharum spp . hybrid cv.…”

Section: Methodsmentioning

confidence: 99%

“…Following bombardment, putative transformed calli were selected on growth media containing 45 mgÁl À1 geneticin in the dark for 8 weeks. In vitro plantlets were regenerated from surviving calli through somatic embryogenesis under a 16-h light:8h dark photoperiod using cool white fluorescent lamps (50 lmol photons m À2 Ás À1 of photosynthetically active radiation; Osram, L 58V/740) for 6-10 weeks (Snyman 2004). Regenerated plantlets were hardened-off in the glasshouse at 26 AE 2 °C under natural light in 20-cm pots containing a potting mix consisting of 2:1:1 parts palm peat, vermiculite (Rosarium, Cape Town, South Africa) and sand.…”

Section: Sugarcane Callus Regeneration and Transformation With The At...mentioning

confidence: 99%

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Overexpression of the Small Ubiquitin‐Like Modifier protease OTS1 gene enhances drought tolerance in sugarcane (Saccharum spp. hybrid)

Masoabi,

Burger,

Botha

et al. 2023

Plant Biol J

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Sugarcane is an economically important crop plant across the globe as it is the primary source of sugar and biofuel. Its growth and development are greatly influenced by water availability; therefore, in periods of water scarcity, yields are severely compromised. Small Ubiquitin‐Like Modifier (SUMO) proteases play an important role in stress responses by regulating the SUMO‐related post‐translational modification of proteins. In an attempt to enhance drought tolerance in sugarcane, this crop was genetically transformed with a cysteine protease (OVERLY TOLERANT TO SALT‐1; OTS1) from Arabidopsis thaliana using particle bombardment. Transgenic plants were analysed in terms of photosynthetic capacity, oxidative damage, antioxidant accumulation and the SUMO‐enrich protein profile was assessed. Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT). The AtOTS1 transgenic plants maintained a high relative moisture content and higher photosynthesis rate when compared to the WT. In addition, when the transgene was expressed at high levels, the transformed plants were able to maintain higher stomatal conductance and chlorophyl content under moderate stress compared to the WT. Under severe water deficit stress, the transgenic plants accumulated less malondialdehyde and maintained membrane integrity. SUMOylation of total protein and protease activity was lower in the AtOTS1 transformed plants compared to the WT, with several SUMO‐enriched proteins exclusively expressed in the transgenics when exposed to water deficit stress. SUMOylation of proteins likely influenced various mechanisms contributing to enhanced drought tolerance in sugarcane.

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“…Following the method described by Snyman (2004), sugarcane callus was induced from immature leaf rolls of the Saccharum spp . hybrid cv.…”

Section: Methodsmentioning

confidence: 99%

Section: Sugarcane Callus Regeneration and Transformation With The At...mentioning

confidence: 99%

Overexpression of the Small Ubiquitin‐Like Modifier protease OTS1 gene enhances drought tolerance in sugarcane (Saccharum spp. hybrid)

Masoabi,

Burger,

Botha

et al. 2023

Plant Biol J

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“…In addition, it is used as a raw material for ethanol production in Brazil, which is the largest producer of cane sugar in the world (Snyman, 2004;López-Ortega et al, 2021). Similar to other food crops, the yield of sugarcane is seriously affected by various viral diseases.…”

Section: Introductionmentioning

confidence: 99%

Breeding of virus-resistant transgenic sugarcane by the integration of the Pac1 gene

Wang

Wang²,

Feng³

et al. 2022

Front. Sustain. Food Syst.

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Various RNA viral diseases on sugarcane result in yield loss and decreased sugar content. Breeding new varieties with virus resistance is the main goal of the sugarcane breeding program. Both single-stranded and double-stranded RNA viruses generated a double-stranded RNA replicative form (RF) during the replication cycle progress. While double-stranded RNA-specific ribonuclease (PAC1) encoded by the Pac1 gene (from Schizosaccharomyces pombe) can recognize and degrade double-stranded RNA specifically without any sequence, the expression of PAC1 in transgenic sugarcane may successfully develop virus-resistant sugarcane. In this research, we first expressed the PAC1 in prokaryotic cells. Then, double-stranded RNA RF of sugarcane's streak mosaic virus (SCSMV) was artificially synthesized. The degradation activity of the PAC1 was successfully tested by mixing the PAC1 protein and the double-stranded RNA RF. After that, the Pac1 gene was ligated to a plant expression vector and was then introduced into a virus-sensitive sugarcane cultivar by using Agrobacterium tumefaciens-mediated transformation method. Transgenic plants were challenged by inoculating with SCSMV. Results showed that although all the transgenic lines were infected by SCSMV, the mosaic symptoms that appeared on the leaves were significantly milder than that of the wild type. All transgenic shoots showed significantly lower viral loads and attained greater heights than wild-type shoots. This research provided a new pathway for breeding new varieties of sugarcane with virus resistance.

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Sugarcane Biotechnology: Axenic Culture, Gene Transfer, and Transgene Expression

Birch¹

2013

Sugarcane: Physiology, Biochemistry, and Functional Biology

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Sugarcane Transformation

Cited by 17 publications

References 42 publications

Overexpression of the Small Ubiquitin‐Like Modifier protease OTS1 gene enhances drought tolerance in sugarcane (Saccharum spp. hybrid)

Overexpression of the Small Ubiquitin‐Like Modifier protease OTS1 gene enhances drought tolerance in sugarcane (Saccharum spp. hybrid)

Breeding of virus-resistant transgenic sugarcane by the integration of the Pac1 gene

Sugarcane Biotechnology: Axenic Culture, Gene Transfer, and Transgene Expression

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