Suicide gene strategies applied in ovarian cancer studies

Nguyen, Quoc Manh; Dupré, Pierre‐François; Haute, Tanguy; Montier, Tristan; d’Arbonneau, Frédérique

doi:10.1038/s41417-023-00590-6

Cited by 6 publications

(5 citation statements)

References 107 publications

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“…Various suicide gene candidates cause host cell death by directly inducing apoptosis, or indirectly by converting prodrugs to toxic metabolites. 32…”

Section: Bacteriophage Applications In Medical Oncologymentioning

confidence: 99%

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New role of bacteriophages in medical oncology

Moradi,

Ghaleh,

Bolandian

et al. 2023

Biotech and App Biochem

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Targeted treatment of cancer is one of the most paramount approaches in cancer treatment. Despite significant advances in cancer diagnosis and treatment methods, there are still significant limitations and disadvantages in the field, including high costs, toxicity, and unwanted damage to healthy cells. The phage display technique is an innovative method for designing carriers containing exogenic peptides with cancer diagnostic and therapeutic properties. Bacteriophages possess unique properties making them effective in cancer treatment. These characteristics include the small size enabling them to penetrate vessels; having no pathogenicity to mammals; easy manipulation of their genetic information and surface proteins to introduce vaccines and drugs to cancer tissues; lower cost of large‐scale production; and greater stimulation of the immune system. Bacteriophages will certainly play a more effective role in the future of medical oncology; however, studies are in the early stages of conception and require more extensive research. We aimed in this review to provide some related examples and bring insights into the potential of phages as targeted vectors for use in cancer diagnosis and treatment, especially regarding their capability in gene and drug delivery to cancer target cells, determination of tumor markers, and vaccine design to stimulate anticancer immunity.

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“…Various suicide gene candidates cause host cell death by directly inducing apoptosis, or indirectly by converting prodrugs to toxic metabolites. 32…”

Section: Bacteriophage Applications In Medical Oncologymentioning

confidence: 99%

“…Phage can be used as a tool for “suicide gene therapy,” the most commonly used approach for solid tumors. Various suicide gene candidates cause host cell death by directly inducing apoptosis, or indirectly by converting prodrugs to toxic metabolites 32 …”

Section: Bacteriophage Applications In Medical Oncologymentioning

confidence: 99%

New role of bacteriophages in medical oncology

Moradi,

Ghaleh,

Bolandian

et al. 2023

Biotech and App Biochem

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“…Particularly, this system contemplates the delivery (with viral or non-viral vectors) of a transgene (viral or bacterial) into tumor cells in order to activate a prodrug or to express the “suicide” gene product in the targeted tumor cells (Fig. 5 ) [ 226 ]. However, this strategy is not completely free from toxic effects.…”

Section: Genome Editing and Hypoxia-response Pathwaymentioning

confidence: 99%

Genome editing and cancer therapy: handling the hypoxia-responsive pathway as a promising strategy

Stampone

Bencivenga

Capellupo

et al. 2023

Cell. Mol. Life Sci.

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The precise characterization of oxygen-sensing pathways and the identification of pO2-regulated gene expression are both issues of critical importance. The O2-sensing system plays crucial roles in almost all the pivotal human processes, including the stem cell specification, the growth and development of tissues (such as embryogenesis), the modulation of intermediate metabolism (including the shift of the glucose metabolism from oxidative to anaerobic ATP production and vice versa), and the control of blood pressure. The solid cancer microenvironment is characterized by low oxygen levels and by the consequent activation of the hypoxia response that, in turn, allows a complex adaptive response characterized mainly by neoangiogenesis and metabolic reprogramming. Recently, incredible advances in molecular genetic methodologies allowed the genome editing with high efficiency and, above all, the precise identification of target cells/tissues. These new possibilities and the knowledge of the mechanisms of adaptation to hypoxia suggest the effective development of new therapeutic approaches based on the manipulation, targeting, and exploitation of the oxygen-sensor system molecular mechanisms.

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“…TRAIL induces cell death upon ligation with its death receptors (Singh et al, 2021). The discovery of its potential to explicitly target malignant cells while sparing normal cells has been met with great enthusiasm, even though the underlying mechanisms have not yet been thoroughly understood (Nguyen et al, 2023). It has been reported that most primary and residual ovarian tumors expressed at least one TRAIL death receptor (DR4 and DR5), while in residual tumors following chemotherapy, one of the two death receptors of TRAIL—DR5 was more frequently observed (Arts et al, 2004; Dong et al, 2008; Duiker et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Two targeted therapies have been approved by FDA and EMA, namely, poly‐ADP‐ribose polymerase inhibitors and anti‐vascular endothelial growth factor monoclonal antibodies (Guan & Lu, 2018). In recent years, cancer gene therapy has also attracted great attention owing to the continued progress made in nucleic acids delivery techniques (Nguyen et al, 2023). However, the application of gene therapy as monotherapy in the treatment of ovarian cancer has not been as successful as had been expected (Áyen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

BSV163/DOPE‐mediated TRAIL gene transfection acts synergistically with chemotherapy against cisplatin‐resistant ovarian cancer

Nguyen,

Dupré,

Berchel

et al. 2023

Chem Biol Drug Des

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Ovarian cancer is the seventh most frequently diagnosed cancer among women worldwide. Most patients experience recurrence and succumb eventually to resistant disease, underscoring the need for an alternative treatment option. In the presented manuscript, we investigated the effect of the TRAIL‐gene, transfected by an innovative bioinspired lipid vector BSV163/DOPE in the presence or absence of cisplatin, to fight against sensitive and resistant ovarian cancer. We showed that BSV163/DOPE can transfect ovarian cancer cell lines (Caov3, OVCAR3, and our new cisplatin‐resistant, CR‐Caov3) safely and efficiently. In addition, TRAIL‐gene transfection in association with cisplatin inhibited cellular growth more efficiently (nearly 50% in Caov3 cells after the combined treatment, and 15% or 25% by each treatment alone, respectively) owing to an increase in apoptosis rate, caspases activity and TRAIL's death receptors expression. Most importantly, such synergistic effect was also observed in CR‐Caov3 cells demonstrated by an apoptosis rate of 35% following the combined treatment in comparison with 17% after TRAIL‐gene transfection or 6% after cisplatin exposition. These results suggest this combination may have potential application for sensitive as well as refractory ovarian cancer patients.

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Suicide gene strategies applied in ovarian cancer studies

Cited by 6 publications

References 107 publications

New role of bacteriophages in medical oncology

New role of bacteriophages in medical oncology

Genome editing and cancer therapy: handling the hypoxia-responsive pathway as a promising strategy

BSV163/DOPE‐mediated TRAIL gene transfection acts synergistically with chemotherapy against cisplatin‐resistant ovarian cancer

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