Suitability of agricultural by-products as production medium for spore production by Beauveria bassiana HQ917687

Abstract

Purpose The seemingly harmful effect of chemical insecticides to the environment, plants and human health, has led to prominent role of entomopathogenic fungi (ENPF) as biopesticide. However, the success of ENPF as biocontrol agent depends on their amenability to easy and cheap mass multiplication. Solid state fermentation (SSF) has emerged as an appropriate technology for management of agro-industrial residues and for their value addition. In this study, the potentiality of agricultural by-products for mass multiplication of Beauveria bassiana was determined.Methods Two sets of SSF experiments with agricultural by-products [tea leaf waste, wheat bran, husk of rice, pigeon pea and urad, and seed cake of jatropha and pongamia] as substrates; one with fortification of nutrients, while other with no nutrient addition were performed. The substrates (200 g) were inoculated with B. bassiana spore suspension (1 9 10 8 spores/ml) and incubated at 28°C for 14 days. After the incubation period, B. bassiana spore concentrations were enumerated. Results Maximum spore production was observed in rice husk, while jatropha seed cake showed least spore production. Addition of nutrient supplements in the substrates showed slight (pongamia seed cake) to significant (rice husk, tea leaf waste) increase in spore production. The results showed constant increase in spore production with increase in C/N ratio of the substrates.Conclusions Mass production processes directly influences the cost, shelf life, virulence, and field efficacy of fungal pathogens. The results from this investigation are expected to pave the way towards commercialization of B. bassiana by augmenting the research in fungal mass production.

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

“…Contrarily, spore production of a biocontrol agent C. rosea on wheat bran and maize meal in a new developed SSF reactor showed an efficient yield of 3.36 × 10 10 spores·g −1 DM, which was 10 times higher than in a conventional tray reactor [159]. Several agricultural wastes such as rice husk, tea leaf waste, and wheat bran have revealed great sporulation of Beauveria bassiana in SSF that presents pathogenicity towards the larvae M. domestica [160].…”

Production of proteases from organic wastes by solid-state fermentation: downstream and zero waste strategies

“…(Eshetu et al 2013), 36 (Pan-In and Sukasen 2016), 19.49 and 2.46 (Mishra et al 2016) respectively, HS and SF present a high content of proteins (nitrogen). Comparing the reported values of protease production in the mentioned examples, and after optimization of the experimental conditions in all cases, maximum production correspond to Jatropha curcas, with a range of production near to 1500 U mL -1 , which was the residue that presented lowest C/N ratio, while the range of production for wheat bran and the mixture of coffee pulp and corn cobs were U mL -1 and 582 U mL -1 respectively.…”

Alternatives for the production of microbial biopesticides based on fungi: the case of Latin America and the Caribbean

“…Diversas publicaciones (Castillo-Lopez et al, 2014;Jenkins et al, 1998;Kobori et al, 2015;Mascarin et al, 2014;Michel-Aceves et al, 2008;Mishra et al, 2016;Xie et al, 2016) abordan la producción masiva de hongos para control biológico, aunque la mayoría describe investigaciones sobre la optimización de la producción de conidios para una cepa específica en un determinado sustrato, pero pocas veces van más allá del nivel de laboratorio. También se hace difícil la comparación entre los procesos de producción que se publican debido a las diferencias en la forma en que se reportan los resultados obtenidos.…”