Suitability of bovine bile compared to urine for detection of free, sulfate and glucuronate boldenone, androstadienedione, cortisol, cortisone, prednisolone, prednisone and dexamethasone by LC–MS/MS

Chiesa, Luca Maria; Nobile, Maria; Panseri, Sara; Vigo, D.; Pavlović, Radmila; Arioli, Francesco

doi:10.1016/j.foodchem.2015.04.131

Cited by 14 publications

(4 citation statements)

References 29 publications

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“…To the best of our knowledge, the immunosensor for DXM through competitive PEC method was proposed for the first time. Compared with the reported detection method 26 27 28 , this assay is more sensitive, inexpensive and time-saving.…”

mentioning

confidence: 99%

A competitive photoelectrochemical immunosensor based on a CdS-induced signal amplification strategy for the ultrasensitive detection of dexamethasone

Wang

Yan

et al. 2015

Sci Rep

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A novel photoelectrochemical immunosensor based on the competitive strategy is proposed for the specific detection of dexamethasone (DXM). Graphitic carbon nitride coupled with bismuth sulfide are used as the sensing matrix for the immobilization of BSA-DXM on the electrode surface, while cadmium sulfide functionalized titanium dioxide (TiO2@CdS) is used as the photoelectric active labels of anti-DXM. Due to the perfect matching of energy levels between TiO2 and CdS, the in situ prepared composite labels show excellent photocurrent response under visible lights. The competitive binding of DXM in sample solutions and BSA-DXM on the electrode surface reduces the specific attachment of labels to the electrode, resulting in a decrease of the photocurrent intensity. Greatly enhanced sensitivity is achieved after the optimization of the detection conditions. Under the optimal detection condition, the well-designed immunosensor for DXM exhibits a low detection limit of 2 pg∙mL−1. Additionally, the proposed immunoassay system shows high specificity, good reproducibility and acceptable stability, which is also expected to become a promising platform for the detection of other small molecules.

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mentioning

confidence: 99%

A competitive photoelectrochemical immunosensor based on a CdS-induced signal amplification strategy for the ultrasensitive detection of dexamethasone

Wang

Yan

et al. 2015

Sci Rep

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“…The authors found that cortisol, cortisone, pseudoendogenous prednisolone, and prednisone are present at higher concentrations, up to seven times higher, in the urine. On the other hand, dexamethasone was found in 10 out of 53 bovine bile samples, but only in one urine sample [ 23 ].…”

Section: Revealing Cortisol In the Agri-food Sectormentioning

confidence: 99%

“…LC-MS is a powerful tool used to determine cortisol levels in food samples due to its high sensitivity, selectivity, specificity, and automation, allowing the analysis of multiple steroids without the need for a previous hydrolysis or derivatization step. The application of LC-MS or tandem mass spectrometry (MS-MS) is the technique of choice for measuring steroid hormones, as has been reported [ 12 , 17 , 18 , 22 , 23 ]. Additionally, some studies reported the utilization of MS with electrospray ionization (ESI), atmospheric pressure photoionization (APPI), time-of-flight (TOF) mass spectrometry [ 10 , 19 , 27 ], or high-performance liquid chromatography (HPLC) [ 25 , 30 ].…”

Section: Revealing Cortisol In the Agri-food Sectormentioning

confidence: 99%

The Importance of Monitoring Cortisol in the Agri-Food Sector—A Systematic Review

2023

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Cortisol monitoring in the agri-food sector is considered a valuable tool due to its direct correlation with growth, reproduction, the immune system, and overall animal welfare. Strategies to monitor this stress hormone and its correlation to food quality and security have been studied in fish farming and the livestock industry. This review discusses studies on monitoring cortisol in the food industry for the first time. The impact of cortisol on animal production, quality, and the security of food products, and the analytical procedures commonly implemented for sample pre-concentration and quantification by liquid chromatography coupled to mass spectrometry, are reviewed and discussed according to the results published in the period 2012–2022. Aquaculture, or fish farming, is the leading agri-food sector, where cortisol’s impact and usefulness are better known than in livestock. The determination of cortisol in fish not only allows for an increase in the production rate, but also the ability to monitor the water quality, enhancing the sustainable development of this industry. In cattle, further studies are needed since it has mainly been used to detect the administration of illicit substances. Current analytical control and monitoring techniques are expensive and often depend on invasive sampling, not allowing fast or real-time monitoring.

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“…8 It is also prohibited for use as a growth promoter and is usually used in association with β-agonists. 10 HC is a synthetic and naturally occurring glucocorticoid, which has anti-inflammatory, immunosuppressive, anti-allergic, and anti-shock effects. 11 17α-Hydoxyprogesterone (OHP) is a derivative of progesterone transformed by 17α-hydroxylase.…”

Section: Introductionmentioning

confidence: 99%

Development, optimization and validation of modified QuEChERS based UPLC-MS/MS for simultaneous determination of nine steroid hormones in milk powder and milk

Zhang

et al. 2022

New J. Chem.

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Suitability of bovine bile compared to urine for detection of free, sulfate and glucuronate boldenone, androstadienedione, cortisol, cortisone, prednisolone, prednisone and dexamethasone by LC–MS/MS

Cited by 14 publications

References 29 publications

A competitive photoelectrochemical immunosensor based on a CdS-induced signal amplification strategy for the ultrasensitive detection of dexamethasone

A competitive photoelectrochemical immunosensor based on a CdS-induced signal amplification strategy for the ultrasensitive detection of dexamethasone

The Importance of Monitoring Cortisol in the Agri-Food Sector—A Systematic Review

Development, optimization and validation of modified QuEChERS based UPLC-MS/MS for simultaneous determination of nine steroid hormones in milk powder and milk

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