Suitability of salivary leucocytes to assess DNA repair ability in human biomonitoring studies by the challenge-comet assay

Fernández-Bertólez, Natalia; Lema-Arranz, Carlota; Fraga, Sónia; Teixeira, João Paulo; Pásaro, Eduardo; Lorenzo-López, Laura; Valdiglesias, Vanessa; Laffón, Blanca

doi:10.1016/j.chemosphere.2022.136139

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…9 Genomic damage assessed by the comet test is repairable. 44 Therefore, this genetic alteration may not be fixed in the cellular genome, bringing less harm to the organism. 9,29 The evaluation of the animals' final weight indicated no change in the final weight or the absolute and relative weight of the heart, lung, liver, kidneys, and spleen compared to experimental and negative control groups.…”

Section: Discussionmentioning

confidence: 99%

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Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Correa,

das Neves,

Oliveira

et al. 2024

Chem. Res. Toxicol.

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Resorcinolic lipids are described as potential examples of selective chemotherapeutic adjuvants that can enhance the effects of cyclophosphamide (CYC) while promoting cell death without causing DNA damage. Therefore, the current study attempted to describe how the resorcinolic lipid methyl 3,5-dimethoxy-2-octanoylbenzoate (AMS35BB) interacted with DNA (DNA docking) and how this compound affected genetic toxicology models and other biological characteristics when combined with CYC. We observed that AMS35BB, used alone (7.5 and 10 mg/kg), increases the frequency of genomic damage (comet assay) but not chromosomal damage (micronuclei assay), lowers phagocytosis, and promotes cell death in Swiss male mice. When used in association with CYC, AMS35BB can reduce the risk of genomic damage by up to 33.8% as well as chromosomal damage, splenic phagocytosis, cell death, and lymphocyte frequency. Molecular docking showed that AMS35BB had a higher affinity than the active metabolite of CYC for binding to the DNA double helix major groove. As a result, AMS35BB has the potential to be both an adjuvant when used in association with CYC and a therapeutic candidate for the development of a selective chemotherapeutic drug.

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Section: Discussionmentioning

confidence: 99%

“…We also compare the effectiveness of AMS35BB with their analogs AMS35AA and AMS049 . Genomic damage assessed by the comet test is repairable . Therefore, this genetic alteration may not be fixed in the cellular genome, bringing less harm to the organism. , …”

Section: Discussionmentioning

confidence: 99%

Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Correa,

das Neves,

Oliveira

et al. 2024

Chem. Res. Toxicol.

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Toxicity of zinc oxide nanoparticles: Cellular and behavioural effects

Fernández-Bertólez,

Alba-González,

Touzani

et al. 2024

Chemosphere

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PERSPECTIVE: The comet assay as a method for assessing DNA damage in cryopreserved samples

Plitta-Michalak,

Ramos,

Stępień

et al. 2024

cryo letters

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The preservation of the nuclear genome's integrity is paramount for the viability and overall health of cells, tissues, and organisms. DNA, being susceptible to damage under physiological conditions and vulnerable to both endogenous and environmental factors, faces constant threats. To assess DNA damage and repair within individual eukaryotic cells, the comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, and highly sensitive method. Originally designed to monitor DNA damage and repair within populations of mammalian cells, the comet assay has now found applications across diverse domains, including yeast, protozoa, plants, and invertebrates. This technique has proven invaluable in cryopreservation studies, serving as a valuable adjunct for determining suitable cryopreservation protocols. These protocols encompass choices related to cryoprotectants, sample preparation, as well as storage conditions in terms of time and temperature. In the realm of animal cryopreservation research, the comet assay stands as a gold-standard method for assessing DNA integrity. Nevertheless, when applied in plant-oriented investigations, additional efforts are essential due to the distinct nature of plant cells and associated technical challenges. This review elucidates the fundamental principles underlying the comet assay, discusses its current iterations, and delineates its applications in the cryopreservation of both animal and plant specimens. Moreover, we delve into the primary challenges confronting the comet assay's utility as a monitoring tool in the context of plant sample cryopreservation.

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Suitability of salivary leucocytes to assess DNA repair ability in human biomonitoring studies by the challenge-comet assay

Cited by 3 publications

References 45 publications

Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Toxicity of zinc oxide nanoparticles: Cellular and behavioural effects

PERSPECTIVE: The comet assay as a method for assessing DNA damage in cryopreserved samples

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