Suitable Technological Conditions for Enzymatic Hydrolysis of Waste Paper by Novozymes® Enzymes NS50013 and NS50010

Abstract

Waste paper belongs to a group of quantitatively the most produced waste types. Enzymatic hydrolysis is becoming a suitable way to treat this type of waste and at the same time, to produce a valuable liquid biofuel, because reducing sugars solutions that are formed during the process of saccharification can be a precursor for following or simultaneous fermentation. If it will be possible to make the enzymatic hydrolysis of the waste paper economically viable, it could serve as one of the new ways to lower the dependence of the transport sector on oil in the future. Only several studies comparing the enzymatic hydrolysis of different waste papers were performed in the past; they are summarized in this manuscript. In our experimental trials, suitable technological conditions for waste paper enzymatic hydrolysis using enzymes from Novozymes® biomass kit: enzymes NS50013 and NS50010 were investigated. The following enzymatic hydrolysis parameters in laboratory scale trials were verified on high cellulose content substrates-filter paper and cellulose pulp: type of buffer, pH, temperature, concentration of the substrate, loading of the enzyme and rate of stirring.

Pretreatment of Crop Residues by Application of Microwave Heating and Alkaline Solution for Biofuel Processing: A Review

“…Maitan-Alfenas et al [52] reported that microorganisms are essential in enzyme production for lignocellulosic biomass saccharification. The saccharification process in the ethanol conversion requires less energy and is done in mild conditions at pH of 5.2-6.2 and a temperature range of 45-50 C [53,54]. There are three distinct major types of cellulase enzymes used in the process: (1) endoglucanases (E C 3.2.1.4) hydrolyze at random internal β-1, 4-glucosidic linkages in the cellulose chain producing oligosaccharides of different lengths and with a shorter chain appearance; (2) exoglucanases of cellobiohydrolases (E C 3.2.1.91) progress along cellulose chain ends and release major products as cellulose or glucose; and (3) β-glucosidases known as β-glucoside glucohydrolases (E C 3.2.1.21) hydrolyze cellulose to glucose, liberate cellobiose, soluble cellodextrins to glucose [12,55].…”