Sulcular polymorphonuclear leucocytes and gingival exudate during experimental gingivitis in man

Kowashi, Yusuke; Jaccard, F; Cimasoni, G.

doi:10.1111/j.1600-0765.1980.tb00269.x

Cited by 96 publications

(82 citation statements)

References 12 publications

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“…It seems logical that serum will provide the basic antioxidant profile of crevicular fluid, as there is constant flow of fluid from the blood into the gingival crevice [19]. However, the finding that the concentrations of eGPx in GCF are significantly greater than those in paired samples of serum suggests a possible local eGPx synthesis and/or storage within the periodontium.…”

Section: Discussioncontrasting

confidence: 50%

Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

2012

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Abstract.Background: Plasma glutathione peroxidase (eGPx) is an important selenium containing antioxidant in human defense against oxidative stress. While crevicular fluid (GCF) eGPx levels and its association with periodontal disease is well documented, there is no data on correlation of GCF and serum eGPx levels in chronic periodontitis. Hence this study was undertaken to further probe into the role of oxidative stress in periodontal diseases and effect of nonsurgical periodontal therapy (NSPT) by correlating GCF and serum levels of eGPx. Materials and methods: Thirty subjects (16-Males and 14-Females; age: 30-38 years) participated in the study. The subjects were divided, based on gingival index, probing pocket depth and clinical attachment level into: Healthy (group-1, n = 10), Gingivitis (group-2, n = 10) and Periodontitis (group-3, n = 10). Chronic periodontitis patients after NSPT constituted group 4. GCF and serum samples collected from each subject were quantified for eGPx levels using Enzyme linked Immunosorbent Assay. Results: The mean eGPx concentrations increased from health (14.01 ng/µl and 78.26 ng/ml) to gingivitis (22.86 ng/µl and 90.44 ng/ml) and then to periodontitis (29.89 ng/µl and 103.43 ng/ml), in GCF and serum respectively. After NSPT, there was statistically significant reduction in eGPx concentration in GCF and serum (19.41 ng/µl and 85.21 ng/ml). Further, all the GCF eGPx values showed a positive correlation to that of serum eGPx level. Conclusion: Thus, increased eGPx concentration in GCF can be considered as an indicator of local increase in oxidative stress. While, increase in serum eGPx levels indicates that periodontal disease can also lead to increased oxidative stress at the systemic level.

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Section: Discussioncontrasting

confidence: 50%

Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

2012

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“…Moreover, the number of leucocytes migrating to the gingival sulcus and periodontal pockets increases during the progression of inflammation [9,10]. While most neutrophils recruited into the gingival tissues migrate to the gingival epithelium and sulcus, the majority of the mononuclear cells, including monocytes/macrophages, T lymphocytes and B cells persist in the connective tissue and form the local cell infiltrate [11].…”

Section: Introductionmentioning

confidence: 99%

Porphyromonas gingivalis-induced inflammatory mediator profile in anex vivohuman whole blood model

Bodet

Chandad

Grenier

2005

Clinical and Experimental Immunology

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SummaryPeriodontitis is characterized by an accumulation of inflammatory cells in periodontal tissue and subgingival sites. Leukocytes play a major role in the host response to Porphyromonas gingivalis , a major aetiological agent of chronic periodontitis. Secretion of high levels of inflammatory mediators, including cytokines and prostaglandins, by leucocytes is believed to contribute to periodontal tissue destruction. The aim of this study was to investigate the inflammatory response of an ex vivo whole blood model to P. gingivalis stimulation. The production of interleukin-1 beta (IL-1β β β β ), IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, tumour necrosis factor alpha (TNF-α α α α ), interferon gamma (IFN-γ γ γ γ ), IFN-γ γ γ γ -inducible protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), Regulated on Activation Normal T cell Expressed and Secreted (RANTES) and prostaglandin E 2 (PGE 2 ) were quantified by enzymelinked immunosorbent assays. P. gingivalis induced the secretion of the proinflammatory cytokines IL-1β β β β , TNF-α α α α , IL-6 and IFN-γ γ γ γ , the chemokines IL-8, RANTES and MCP-1 and the inflammatory mediator PGE 2 in an ex vivo human whole blood model. The secretion levels were dependent on the strain and the infectious dose used. While the mediator profiles were comparable between six healthy subjects, a high interindividual variability in the levels of secreted mediators was observed. This study supports the view that P. gingivalis , by inducing high levels of inflammatory mediators from a mixed leucocyte population, can contribute to the progression of periodontitis.

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“…Large accumulations of PMN in the tissues during gingival inflammation have been reported. [12][13][14] These PMNs can release lysosomal substances which not only may have significant effects on microbiota and host tissues but also provide important marker(s) for inflammation of the periodontal tissue.…”

Section: Introductionmentioning

confidence: 99%

Gingival crevicular fluid lactoferrin levels in adult per iodontitis patients

Tsai

Kao

1998

Australian Dental Journal

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The present study was designed to determine in a cross-sectional study whether there was any relationship between the levels of lactoferrin in gingival crevicular fluid and clinical periodontal parameters. Crevicular fluid was collected from individual sites using standardized filter paper strips (clinically healthy sites, N=23; periodontitis sites, n=66) and evaluated for lactoferrin by enzyme-linked immunosorbent assay. The data showed that: (1) the total amounts of lactoferrin were 0.003-0.021 ng (30 second sample) (average 0.009Ϯ0.005 ng) in a clinically healthy periodontium group and 0.016-3.847 ng (30 second sample) (average 0 . 5 7 5Ϯ0.069 ng) in adult periodontitis patients (statistically significantly higher in adult periodontitis patients); and (2) the total amounts of lactoferrin were significantly correlated with clinical parameters, especially a strong positive correlation with gingival crevicular fluid volume (r=0.85, p<0.01) and with probing depth (r=0.71, p<0.01). These results indicated that quantification of lactoferrin in gingival crevicular fluid may be a more sensitive indicator of periodontal pathology than traditional clinical indices.

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Sulcular polymorphonuclear leucocytes and gingival exudate during experimental gingivitis in man

Cited by 96 publications

References 12 publications

Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

Porphyromonas gingivalis-induced inflammatory mediator profile in anex vivohuman whole blood model

Gingival crevicular fluid lactoferrin levels in adult per iodontitis patients

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