Sulfate permeasesphylogenetic diversity of sulfate transport.

Piłsyk, Sebastian; Paszewski, Andrzej

doi:10.18388/abp.2009_2470

Cited by 42 publications

(36 citation statements)

References 90 publications

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“…With respect to the mode of sulfate acquisition by MSR, sulfate transport in sulfate-reducing micro-organisms studied to date is strictly regulated and is accomplished via numerous possible mechanisms. These include ATP-dependent ABC-type active transporters that pump sulfate into the cell against a concentration gradient (Piłsyk & Paszewski, 2009) and are homologous to Fig. 3 Comparison between our data (D. vulgaris in red and D. alaskensis in blue) and those generated in a semi-continuous-culture apparatus by Habicht et al (2002Habicht et al ( , 2005 (in black symbols), along with values from D. desulphuricans (green) from Harrison & Thode (1958), from closed-system experiments.…”

Section: Evaluation Of Cellular K S As a Predictor Of Fractionationmentioning

confidence: 75%

“…With respect to the mode of sulfate acquisition by MSR, sulfate transport in sulfate‐reducing micro‐organisms studied to date is strictly regulated and is accomplished via numerous possible mechanisms. These include ATP‐dependent ABC‐type active transporters that pump sulfate into the cell against a concentration gradient (Piłsyk & Paszewski, ) and are homologous to enzymes for assimilatory sulfate transport in other (non‐sulfate‐reducing) micro‐organisms, as well as H + and Na + symporters, which rely on concentration gradients and do not require ATP (Cypionka, ). Energetic considerations favor the symporters as the primary transport mechanism for dissimilatory metabolism (Cypionka, ), though symporters cannot produce intracellular substrate concentrations that vary from the environment.…”

Section: Resultsmentioning

confidence: 99%

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Patterns of sulfur isotope fractionation during microbial sulfate reduction

et al. 2015

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These data reveal complexity in the sulfate concentration-fractionation relationship. 42Sulfur isotope fractionation during sulfate reduction relates to environmental sulfate 43 concentrations but also to strain-specific physiological parameters such as the affinity of sulfate-44 reducing microorganisms for sulfate and electron donors. Previous studies have suggested that 45 the relationship between sulfate concentration and isotope fractionation is best fit with a MM fit. 46suggested We present a simple model, grounded in the physiology of sulfate reduction, in which 47 the ratio of MM relationships for sulfate and electron donor uptake produces the relationships 48 seen in experimental studies: a MM relationship with sulfate concentration, and a hyperbolic 49 relationship with growth rate. 50Since both environmental and biological factors influence the fractionation recorded in 51 geological samples, understanding their relationship is critical to interpreting the sulfur isotope 52 record. As the acquisition machinery for sulfate and electron acquisition has been subject to 53 selective pressure over Earth history, its evolution may complicate efforts to uniquely reconstruct 54 ambient sulfate concentrations from a single sulfur isotopic composition. 55Patterns of SRB S-isotope fractionation 2 56

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Section: Evaluation Of Cellular K S As a Predictor Of Fractionationmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Patterns of sulfur isotope fractionation during microbial sulfate reduction

et al. 2015

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“…In the genome of UW4, six SulP family sulfate transporters were identified (PputUW4_00023, 00047, 00617, 02916, 03092, 04194). Although the role of these transporters in sulfate assimilation in bacteria is not clear, the homologs in several eukaryotes have been characterized and shown to be active components of sulfate transport, some of which function as sulfate:H + symporters [91]. Organosulfur compounds are widely present in nature.…”

Section: Resultsmentioning

confidence: 99%

The Complete Genome Sequence of the Plant Growth-Promoting Bacterium Pseudomonas sp. UW4

et al. 2013

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The plant growth-promoting bacterium (PGPB) Pseudomonas sp. UW4, previously isolated from the rhizosphere of common reeds growing on the campus of the University of Waterloo, promotes plant growth in the presence of different environmental stresses, such as flooding, high concentrations of salt, cold, heavy metals, drought and phytopathogens. In this work, the genome sequence of UW4 was obtained by pyrosequencing and the gaps between the contigs were closed by directed PCR. The P. sp. UW4 genome contains a single circular chromosome that is 6,183,388 bp with a 60.05% G+C content. The bacterial genome contains 5,423 predicted protein-coding sequences that occupy 87.2% of the genome. Nineteen genomic islands (GIs) were predicted and thirty one complete putative insertion sequences were identified. Genes potentially involved in plant growth promotion such as indole-3-acetic acid (IAA) biosynthesis, trehalose production, siderophore production, acetoin synthesis, and phosphate solubilization were determined. Moreover, genes that contribute to the environmental fitness of UW4 were also observed including genes responsible for heavy metal resistance such as nickel, copper, cadmium, zinc, molybdate, cobalt, arsenate, and chromate. Whole-genome comparison with other completely sequenced Pseudomonas strains and phylogeny of four concatenated “housekeeping” genes (16S rRNA, gyrB, rpoB and rpoD) of 128 Pseudomonas strains revealed that UW4 belongs to the fluorescens group, jessenii subgroup.

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“…The leading models (Rees and trithionate pathways) share many features, most of which are derived from early biochemical studies of the sulfate reduction pathway (Hilz & Lipmann, 1955;Robbins & Lipmann, 1955;Bandurski et al, 1956;Peck, 1959Peck, , 1961Peck, , 1962Michaels et al, 1970). There is wide agreement that sulfate is actively transported into the cell via sulfate permease (Piłsyk & Paszewski, 2009). ATP sulfurylase then activates intracellular sulfate to the reactive intermediate APS (Hilz & Lipmann, 1955;Robbins & Lipmann, 1955), which -unlike sulfate -can be readily reduced to sulfite.…”

Section: Previous Models For the Sulfate Reduction Networkmentioning

confidence: 99%

Revisiting the dissimilatory sulfate reduction pathway

2011

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Sulfur isotopes in the geological record integrate a combination of biological and diagenetic influences, but a key control on the ratio of sulfur isotopes in sedimentary materials is the magnitude of isotope fractionation imparted during dissimilatory sulfate reduction. This fractionation is controlled by the flux of sulfur through the network of chemical reactions involved in sulfate reduction and by the isotope effect associated with each of these chemical reactions. Despite its importance, the network of reactions constituting sulfate reduction is not fully understood, with two principle networks underpinning most isotope models. In this study, we build on biochemical data and recently solved crystal structures of enzymes to propose a revised network topology for the flow of sulfur through the sulfate reduction metabolism. This network is highly branched and under certain conditions produces results consistent with the observations that motivated previous sulfate reduction models. Our revised network suggests that there are two main paths to sulfide production: one that involves the production of thionate intermediates, and one that does not. We suggest that a key factor in determining sulfur isotope fractionation associated with sulfate reduction is the ratio of the rate at which electrons are supplied to subunits of Dsr vs. the rate of sulfite delivery to the active site of Dsr. This reaction network may help geochemists to better understand the relationship between the physiology of sulfate reduction and the isotopic record it produces.

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Sulfate permeasesphylogenetic diversity of sulfate transport.

Cited by 42 publications

References 90 publications

Patterns of sulfur isotope fractionation during microbial sulfate reduction

Patterns of sulfur isotope fractionation during microbial sulfate reduction

The Complete Genome Sequence of the Plant Growth-Promoting Bacterium Pseudomonas sp. UW4

Revisiting the dissimilatory sulfate reduction pathway

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