Sulfate reduction and inorganic carbon assimilation in acidic thermal springs of the Kamchatka peninsula

Frolov, Evgenii N.; Merkel, Alexander Y.; Пименов, Н. В.; Khvashchevskaya, A. A.; Bonch-Osmolovskaya, E. A.; Chernykh, N. A.

doi:10.1134/s0026261716040068

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…Here, we studied terrestrial thermal acidic environments in which DSR was previously detected 29 . Using community profiling, culture studies and radioisotope measurements, coupled with comparative genomics, proteomics, and phylogenetics, we conclude that among the reported archaeal sulphate reducers with sequenced genomes, only Archaeoglobus spp.…”

Section: Source_data_figure1xlsxmentioning

confidence: 99%

“…The microbial community of Oreshek Spring (Moutnovsky Volcano, Kamchatka Peninsula, Russia, temperature of 91ºC, pH 3.5, sulphate concentration of 538 mg/l), which displays significant rates of sulphate reduction (1.094 nmol H 2 S/cm 3 •day) 29 , was analysed by sequencing 16S rRNA gene fragments. After quality filtering, we obtained 28152716S rRNA gene fragments corresponding to 434 operational taxonomic units (OTUs) defined with 0.98 similarity threshold.…”

Section: Community Profiling Of Oreshek Springmentioning

confidence: 99%

“…The Vulcanisaeta component of culture 768-28 was given the tentative species name "Candidatus V. moutnovskia" [29][30][31] . We designed specific primers for the detection of "Candidatus V. moutnovskia" dsrAB genes and used them for qPCR analysis of an environmental sample from Oreshek Spring.…”

Section: Community Profiling Of Oreshek Springmentioning

confidence: 99%

“…Sulphate is present in high-temperature submarine, deep subsurface and terrestrial environments where archaeal organisms are abundant. Several independent studies have reported high-temperature sulphate reduction in terrestrial neutral or acidic hot springs of Yellowstone National Park43 and Kamchatka Peninsula29,44,45 , but the species responsible for high-temperature sulphate reduction had not been identified. We report that hyperthermophilic archaea of the genus Vulcanisaeta are one of the dominant groups in the acidic and hot Oreshek Spring.…”

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Dissimilatory sulfate reduction in the archaeon ‘Candidatus Vulcanisaeta moutnovskia’ sheds light on the evolution of sulfur metabolism

et al. 2020

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Extended_Data_Figur e_4.epsError bars represent standard deviations of independent radioisotopic measurement experiments (n = 3).Extended Data Fig.Genes related to sulphate reduction. Extended_Data_Figur e_5.epsThe key sulphate reduction genes from genomes of selected sulphate reducing archaea ("Candidatus V. moutnovskia", Caldivirga sp. Obs2 and A. fulgidus) and bacteria (D. vulgaris Hildenborough and A. degensii) are shown, as well as from genomes of archaea not capable of sulphate reduction (V. distributa, V. souniana, Caldivirga sp. MU80, Ca. Methanodesulfokores washburnensis MDKW and unclassified Aigarchaeota JZ bin 15). Extended Data Fig. Proteomic analysis of the soluble fraction of cells in the binary culture. Extended_Data_Figur e_6.eps Dissimilatory sulphate reduction in archaeon Candidatus Vulcanisaeta moutnovskia sheds light on evolution of sulphur metabolism

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Section: Source_data_figure1xlsxmentioning

confidence: 99%

Section: Community Profiling Of Oreshek Springmentioning

confidence: 99%

Section: Community Profiling Of Oreshek Springmentioning

confidence: 99%

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Dissimilatory sulfate reduction in the archaeon ‘Candidatus Vulcanisaeta moutnovskia’ sheds light on the evolution of sulfur metabolism

et al. 2020

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“…Based on genomic analysis, as well as on proteomic and cultural experiments, it was shown that "Candidatus Vulcanisaeta moutnovskia" isolated from a hot spring at the bottom of Mutnovskii volcano [18,19], was capable of sulfate reduction [5]. The activity of SRP was demonstrated for Termofilnyi Spring [20], Bourlyashchy Pool [21], Oreshek Spring and Oil Site [5,22] by in situ experiments with 35 S-labelled sulfate. The 16S rRNA genes analysis, as well as metagenomic analyses, were performed for microbial communities of Kamchatka hot springs [13,14,[23][24][25][26][27][28][29], but the diversity of SRP in Kamchatka hot springs was never discussed.…”

Section: Introductionmentioning

confidence: 99%

Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs

et al. 2021

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Microbial communities of the Kamchatka Peninsula terrestrial hot springs were studied using radioisotopic and cultural approaches, as well as by the amplification and sequencing of dsrB and 16S rRNA genes fragments. Radioisotopic experiments with 35S-labeled sulfate showed that microbial communities of the Kamchatka hot springs are actively reducing sulfate. Both the cultivation experiments and the results of dsrB and 16S rRNA genes fragments analyses indicated the presence of microorganisms participating in the reductive part of the sulfur cycle. It was found that sulfate-reducing prokaryotes (SRP) belonging to Desulfobacterota, Nitrospirota and Firmicutes phyla inhabited neutral and slightly acidic hot springs, while bacteria of phylum Thermodesulofobiota preferred moderately acidic hot springs. In high-temperature acidic springs sulfate reduction was mediated by archaea of the phylum Crenarchaeota, chemoorganoheterotrophic representatives of genus Vulcanisaeta being the most probable candidates. The 16S rRNA taxonomic profiling showed that in most of the studied communities SRP was present only as a minor component. Only in one microbial community, the representatives of genus Vulcanisaeta comprised a significant group. Thus, in spite of comparatively low sulfate concentrations in terrestrial hot springs of the Kamchatka, phylogenetically and metabolically diverse groups of sulfate-reducing prokaryotes are operating there coupling carbon and sulfur cycles in these habitats.

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Metal sulfide precipitation mediated by an elemental sulfur-reducing thermoacidophilic microbial culture from a full-scale anaerobic reactor

2022

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Sulfate reduction and inorganic carbon assimilation in acidic thermal springs of the Kamchatka peninsula

Cited by 10 publications

References 24 publications

Dissimilatory sulfate reduction in the archaeon ‘Candidatus Vulcanisaeta moutnovskia’ sheds light on the evolution of sulfur metabolism

Dissimilatory sulfate reduction in the archaeon ‘Candidatus Vulcanisaeta moutnovskia’ sheds light on the evolution of sulfur metabolism

Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs

Metal sulfide precipitation mediated by an elemental sulfur-reducing thermoacidophilic microbial culture from a full-scale anaerobic reactor

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