Sulfate transport in Aspergillus nidulans: A novel gene encoding alternative sulfate transporter

Piłsyk, Sebastian; Natorff, Renata; Sieńko, Marzena; Paszewski, Andrzej

doi:10.1016/j.fgb.2006.11.007

Cited by 20 publications

(43 citation statements)

References 37 publications

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“…Moreover, the sB⌬ strain grew poorly on sulfite (SO 3 2Ϫ ) and thiosulfate, suggesting that the SB transporter is also involved in the uptake of partially reduced inorganic S sources. In addition, the deletant became resistant to chromate, a toxic sulfate analogue that is commonly used to isolate and validate mutants impaired in the uptake of sulfate (51,52,59). As expected, AfS177 grew normally on organic S sources such as methionine or cysteine.…”

Section: Resultssupporting

confidence: 56%

“…2A) revealed that the mutant is indeed unable to grow in the presence of SO 4 2Ϫ or nitrophenylsulfate as the sole S source. This demonstrates that, in contrast to A. nidulans (52), the sB-encoded transporter appears to be functionally unique in A. fumigatus. Moreover, the sB⌬ strain grew poorly on sulfite (SO 3 2Ϫ ) and thiosulfate, suggesting that the SB transporter is also involved in the uptake of partially reduced inorganic S sources.…”

Section: Resultsmentioning

confidence: 90%

“…In the past, a specific sulfate transporter was identified in the closely related species Aspergillus nidulans (51). Recent investigations characterized this protein as being a member of the SulP family of sulfate transporters (52,53), whose encoding sB gene (AN2730) was shown to be regulated by MetR (52-54). We previously identified its A. fumigatus orthologue (AFUA_1G05020) in a BLAST query (55)(56)(57) and confirmed that the expression of the encoding gene is regulated by MetR (33).…”

Section: Resultsmentioning

confidence: 99%

“…In fungi, however, high degrees of redundancy for uptake activities are evident, which reflect the osmotrophic feeding style common to these organisms. The fact that the sB gene encodes the sole sulfate transporter in A. fumigatus, as proven by the inability of the respective deletant to grow on sulfate as an S source and its resistance to the toxic analogue chromate, is exceptional in relation to other fungal organisms: Saccharomyces cerevisiae (83), Neurospora crassa (84), and Penicillium chrysogenum (85) appear to contain more than one sulfate transporter, and for the closely related species A. nidulans, an alternative sulfate transporter (GenBank accession number ABA28286.1) encoded by the astA gene has been described (52). A BLAST query failed to identify an AstA ortho- logue in A. fumigatus (not shown), and we therefore conclude that the capacity of A. fumigatus to take up sulfate completely depends on the SB transporter.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant

et al. 2016

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e Fungal infections are of major relevance due to the increased numbers of immunocompromised patients, frequently delayed diagnosis, and limited therapeutics. To date, the growth and nutritional requirements of fungi during infection, which are relevant for invasion of the host, are poorly understood. This is particularly true for invasive pulmonary aspergillosis, as so far, sources of (macro)elements that are exploited during infection have been identified to only a limited extent. Here, we have investigated sulfur (S) utilization by the human-pathogenic mold Aspergillus fumigatus during invasive growth. Our data reveal that inorganic S compounds or taurine is unlikely to serve as an S source during invasive pulmonary aspergillosis since a sulfate transporter mutant strain and a sulfite reductase mutant strain are fully virulent. In contrast, the S-containing amino acid cysteine is limiting for fungal growth, as proven by the reduced virulence of a cysteine auxotroph. Moreover, phenotypic characterization of this strain further revealed the robustness of the subordinate glutathione redox system. Interestingly, we demonstrate that methionine synthase is essential for A. fumigatus virulence, defining the biosynthetic route of this proteinogenic amino acid as a potential antifungal target. In conclusion, we provide novel insights into the nutritional requirements of A. fumigatus during pathogenesis, a prerequisite to understanding and fighting infection.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant

et al. 2016

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“…These same enzymes in A. nidulans, on the other hand, are not down-regulated in response to S metabolites (Clarke et al, 1997;Sienko and Paszewski, 1999;Natorff et al, 2003). The transcription of sulfate permeases is suppressed in all species studied by low concentrations of S metabolites (Bradfield et al, 1970;Ketter and Marzluf, 1988;Ketter et al, 1991;Marzluf, 1993Marzluf, , 1997Cherest et al, 1997;Grynberg et al, 2001;Pilsyk et al, 2007). A putative Cys synthase transcript has been identified from the AM fungus G. intraradices (http://darwin.nmsu.edu/~plammers/glomus/), which appears to be the only report on the identity or regulation of AM fungal genes related to S metabolism, transport, or regulation.…”

Section: Somentioning

confidence: 99%

Sulfur Transfer through an Arbuscular Mycorrhiza

2008

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Despite the importance of sulfur (S) for plant nutrition, the role of the arbuscular mycorrhizal (AM) symbiosis in S uptake has received little attention. ]Met was supplied in the fungal compartment. Fungal transcripts for putative S assimilatory genes were identified, indicating the presence of the trans-sulfuration pathway. The suppression of fungal sulfate transfer in the presence of Cys coincided with a reduction in putative sulfate permease and not sulfate adenylyltransferase transcripts, suggesting a role for fungal transcriptional regulation in S transfer to the host. A testable model is proposed describing root S acquisition through the AM symbiosis.

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The Aspergillus nidulans pigP gene encodes a subunit of GPI-N-acetylglucosaminyltransferase which influences filamentation and protein secretion

Piłsyk

Paszewski

2009

Curr Genet

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Glycosylphosphatidylinositol (GPI) anchoring is the main mechanism allowing proper localization of secretory proteins in cell membranes. We have isolated an Aspergillus nidulans homolog of the human PIG-P gene, which encodes a subunit of acetylglucosaminyltransferase (GPI-GnT)-an enzyme involved in the synthesis of GPI anchors. A. nidulans pigP mutants have significantly decreased GPI synthesis. On solid media they show strong growth retardation (the "button" phenotype) while in liquid minimal media they show overall good growth but with hyperbranched and bulbous hyphae with impaired septation. Furthermore, the pigP strains, in contrast to the wild-type, abundantly secrete a 33-kDa alkaline serine protease (ALP) into the liquid medium.

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Sulfate transport in Aspergillus nidulans: A novel gene encoding alternative sulfate transporter

Cited by 20 publications

References 37 publications

Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant

Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant

Sulfur Transfer through an Arbuscular Mycorrhiza

The Aspergillus nidulans pigP gene encodes a subunit of GPI-N-acetylglucosaminyltransferase which influences filamentation and protein secretion

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