2012
DOI: 10.1016/j.actbio.2012.06.039
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Sulfated hyaluronan/collagen I matrices enhance the osteogenic differentiation of human mesenchymal stromal cells in vitro even in the absence of dexamethasone

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Cited by 62 publications
(105 citation statements)
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References 73 publications
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“…For in vitro studies with human bone marrow stromal cells, dexamethasone is a mandatory supplement inducing osteogenic differentiation, but it is also a component in the adipogenic differentiation media [18,42]. Dex induced the expression of several osteogenic markers as tnap and osad [this study, [43,44]]. Increasing TNAP activity is a prerequisite for increasing phosphate amounts at the site of mineralization and lead in consequence to increasing calcium phosphate deposition.…”
Section: Discussionmentioning
confidence: 94%
See 1 more Smart Citation
“…For in vitro studies with human bone marrow stromal cells, dexamethasone is a mandatory supplement inducing osteogenic differentiation, but it is also a component in the adipogenic differentiation media [18,42]. Dex induced the expression of several osteogenic markers as tnap and osad [this study, [43,44]]. Increasing TNAP activity is a prerequisite for increasing phosphate amounts at the site of mineralization and lead in consequence to increasing calcium phosphate deposition.…”
Section: Discussionmentioning
confidence: 94%
“…Osteoadherin (¼osteomodulin, OSAD) is an extracellular matrix keratan sulfate-containing proteoglycan which is involved in a controlling mineralization process in bones and teeth [45]. Other typical osteogenic markers as collagen typ 1, osterix, and osteocalcin are suppressed by dex [41,43]. In mesenchymal progenitor cells (ROB-C26), a down-regulation of canonical Wnt/β-catenin signaling was seen with dex, decelerating the osteogenic differentiation [46].…”
Section: Discussionmentioning
confidence: 96%
“…The synthesis and characterization of (s)GAG derivatives were performed as described earlier [9, 11, 13]. The preparation of the sulfated HA derivative sHA1Δ6S was previously reported by Becher et al [14] and Schulz et al [15].…”
Section: Methodsmentioning
confidence: 99%
“…Synthetically sulfated hyaluronan derivatives (sHA) and oversulfated chondroitin sulfate derivatives (sCS) as components of artificial ECM (aECM) have been recently described to promote adhesion and proliferation of dermal fibroblasts [9] and to influence osteoclastogenesis [10]. aECM with sHA derivatives are known to enhance osteogenic differentiation of hBMSC even in the absence of dexamethasone [11] which has been described as an established supplement to induce osteogenic differentiation in vitro [12]. …”
Section: Introductionmentioning
confidence: 99%
“…Matrices of Coll type I are well known for promoting cellular adhesion and spreading as well as proliferation of ostoblasts [40,51]. Coll matrices containing sulfated hyaluronan stimulated the osteogenic differentiation of hMSC by increasing alkaline phosphatase expression and activity as well as calcium phosphate deposition in comparison to those containing Coll with and without CS-A/C [52]. At the same time aECM containing sulfated hyaluronan and CS led to a significant inhibition of osteoclast differentiation and resorption, which was found to be depending on their degree of sulfation [53].…”
Section: Artificial Ecm Fabrication and Characterizationmentioning
confidence: 98%