Sulfated polysaccharides from <i>Phaeodactylum tricornutum</i>: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro

Yang, Shengfeng; Wan, Haitao; Wang, Rui; Hao, Daijun

doi:10.7717/peerj.6409

Cited by 32 publications

(20 citation statements)

References 37 publications

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“…In order to improve the technoeconomic feasibility of Phaeodactylum biorefinery, it is important to characterise the carbohydrates which can be converted to bioethanol or biobutanol. The polysaccharides from microalgae have also been shown to have some bioactivities such as antitumor, antibacterial, antioxidant, etc for potential pharmaceutical applications [37]. Thus, the carbohydrate content from the two morphotypes of P. tricornutum cultures were generally determined using the PSA assay, shown in line graphs in showed a plateau at approximately 21% DW in both strains.…”

Section: Carbohydrate Content From Fusiform and Oval P Tricornutum Cell Culturesmentioning

confidence: 99%

Morphological and biochemical changes in Phaeodactylum tricornutum triggered by culture media: Implications for industrial exploitation

2020

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Phaeodactylum tricornutum is a polymorphic marine diatom, displaying three main morphotypes: fusiform, triradiate and oval. It is of great interest for industrial biotechnology as a natural rich source of valuable eicosapentaenoic acid (EPA) and fucoxanthin. Changing culture conditions such as temperature and salinity has been shown to elicit morphological changes in P. tricornutum. However, limited information is available about the conditions that can be used for controlling cell morphology and growth of a particular cell morphotype with high biomass productivity. While the phenomenon of pleiomorphy is intrinsically interesting, there has not been a systematic study linking this behavior to the ability of P. tricornutum to perform as a platform for industrial biotechnology. In this study, the effects of culture medium and culture age on morphological and biochemical changes in P. tricornutum were investigated. Mann and Myers' medium was identified as eliciting significant morphotype conversion from fusiform to oval in P. tricornutum. Liquid cultures containing more than 90% oval cells were obtained and well-maintained in this medium under constant shaking condition, allowing high dry biomass concentration (0.73 g L -1 ) to be achieved. Biochemical composition analyses revealed that higher protein (% dry weight) was obtained from oval cell cultures compared to fusiform cell cultures maintained in f/2 medium over 21 days cultivation. Meanwhile, pigment was markedly accumulated in oval cell cultures whereas lipid and carbohydrate were highly accumulated in fusiform cell cultures. This work offered a novel way to regulate cell morphology of P. tricornutum and provided significant implications for upstream cultivation strategies to optimise manufacture of different classes of product in P. tricornutum.

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Section: Carbohydrate Content From Fusiform and Oval P Tricornutum Cell Culturesmentioning

confidence: 99%

Morphological and biochemical changes in Phaeodactylum tricornutum triggered by culture media: Implications for industrial exploitation

2020

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“…The cells were placed at a density of (1 × 10 5 ) cells/well in a 96 well plate supplemented with 100 µl of MEM medium with 10% of FBS in each well. HepG2 cells along with (D-GalN (50 mmol) were treated with different GC polysaccharide extract concentration (250 and 500 µg mL −1 ) for 8 h and the cell viability was assessed by MTT assay ( Yang et al, 2019 ). Silymarin (100 µg mL −1 ) was used as the reference standard.…”

Section: Methodsmentioning

confidence: 99%

Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides

Alkahtani

Elshikh

Almaary

et al. 2020

Saudi Journal of Biological Sciences

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Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities. The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides. The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability. Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method. Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines. The findings showed that GC polysaccharides MIC were 1.06 and 0.56 mg mL −1 against E. coli and S. aureus, respectively. Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols. The IC 50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent. The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner. GC polysaccharides at a dose of 500 µg ml −1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%). The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.

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“…[62], Tribonema sp. [73], and Phaeodactylum tricornutum [74] against HepG2 cell lines (liver cancer).…”

Section: Functional Characteristicsmentioning

confidence: 99%

Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications

et al. 2021

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Microalgae and cyanobacteria are photosynthetic microorganisms capable of synthesizing several biocompounds, including polysaccharides with antioxidant, antibacterial, and antiviral properties. At the same time that the accumulation of biomolecules occurs, microalgae can use wastewater and gaseous effluents for their growth, mitigating these pollutants. The increase in the production of polysaccharides by microalgae can be achieved mainly through nutritional limitations, stressful conditions, and/or adverse conditions. These compounds are of commercial interest due to their biological and rheological properties, which allow their application in various sectors, such as pharmaceuticals and foods. Thus, to increase the productivity and competitiveness of microalgal polysaccharides with commercial hydrocolloids, the cultivation parameters and extraction/purification processes have been optimized. In this context, this review addresses an overview of the production, characterization, and potential applications of polysaccharides obtained by microalgae and cyanobacteria. Moreover, the main opportunities and challenges in relation to obtaining these compounds are highlighted.

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Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro

Cited by 32 publications

References 37 publications

Morphological and biochemical changes in Phaeodactylum tricornutum triggered by culture media: Implications for industrial exploitation

Morphological and biochemical changes in Phaeodactylum tricornutum triggered by culture media: Implications for industrial exploitation

Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides

Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications

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