Sulfide removal from livestock biogas by Azospirillum-like anaerobic phototrophic bacteria consortium

Silva, M.L.B. da; Mezzari, Melissa P.; Ibelli, A. M. G.; Gregory, Kelvin B.

doi:10.1016/j.ibiod.2013.09.013

Cited by 18 publications

(8 citation statements)

References 10 publications

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“…The PCR products were purified using the PureLink TM PCR purification kit (Invitrogen), cloned into pGEMT Easy Vector Systems (Promega) according to the manufacturer's protocols. The recombinant clones were transformed in E. coli DH5␣ cells and the white colonies were selected and amplified to confirm the vector insert (Silva et al, 2014). The DNA was extracted using the PureLink ® Quick Plasmid Miniprep Kit (Invitrogen), sequenced with an Applied Biosystems ABI Prism 3130 Avant ® genetic analyzer and submitted to BLAST analysis to confirm the sequences obtained (Altschul et al, 1990).…”

Section: Qpcr Reactionsmentioning

confidence: 99%

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.

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Section: Qpcr Reactionsmentioning

confidence: 99%

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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“…Some of the biogas quality requirements of different countries for grid injection [17][18][19][20] Parameter The potential of Azospirillum-like anaerobic phototrophic bacteria consortium for H 2 S removal from swine waste biogas was found to be more than 97%. Rapid decrease in concentration of H 2 S from 1200 to 30 ppm was observed within 3 h. The removal efficiency remains unaffected when the experiment was repeated, indicating the robustness of the biofiltering process [41].…”

Section: Adsorption Processmentioning

confidence: 87%

Upgrading strategies for effective utilization of biogas

Salihu

Alam

2015

Env Prog and Sustain Energy

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Production of biogas is based on anaerobic digestion of different renewable raw materials including human, animal, agricultural, industrial, and municipal wastes. In addition to methane content, biogas contains carbon dioxide along with water vapor, hydrogen sulfide, ammonia, and depending on the raw materials siloxane can be present. Thus, different purification and upgrading strategies are necessary in order to enhance the methane content; this review presents some of the upgrading technologies for practical removal of major contaminants in biogas. Recent development in membrane technology with high selectivity and permeability could serve as a boost in search for the most efficient biogas upgrading process capable of meeting the requirements for its use in vehicle fuel as well as incorporation in the natural gas grid. © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1512–1520, 2015

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“…Moreover, they are responsible for the production and consumption of these gases. In this context, to date, several studies have identified the genus Azospirillum in enriched environments of CO 2 , CH 4 , and H 2 S (Grouzdev et al, 2018; Grover et al, 2015; Lavrinenko et al, 2010; Silva et al, 2014; Wasai & Minamisawa, 2018); however, consumption or degradation of these gases by Azospirillum has not been reported yet. This indicates that Azospirillum might have a natural ability to endure the stressful concentration of greenhouse gases, explaining the high growth capacity, IAA productivity, and yield attained by this bacterium when cultured under the biogas condition.…”

Section: Discussionmentioning

confidence: 99%

“…To date, many studies have demonstrated the beneficial effects of Azospirillum on plant and microalga physiology, mainly induced by IAA production (Cassán et al, 2020;Contreras-Angulo et al, 2019;Pagnussat et al, 2020;Peng et al, 2020). Silva et al (2014) also demonstrated that Azospirillum sp. 5c played an important role during sulfide removal from swine waste biogas, maintaining a syntrophic relationship between the dominant microorganisms and others in the community.…”

Section: F I G U R E 5 Ipdc Gene Expression In Azospirillum Brasilensementioning

confidence: 98%

Active indole-3-acetic acid biosynthesis by the bacterium Azospirillum brasilense cultured under a biogas atmosphere enables its beneficial association with microalgae

Barbosa-Nuñez

Palacios

de-Bashan

et al. 2022

Journal of Applied Microbiology

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Aims: This study assessed, at the physiological and molecular levels, the effect of biogas on indole-3-acetic acid (IAA) biosynthesis by Azospirillum brasilense as well as the impact of this bacterium during CO 2 fixation from biogas by Chlorella vulgaris and Scenedesmus obliquus.Methods and Results: IpdC gene expression, IAA production and the growth of A. brasilense cultured under air (control) and biogas (treatment) were evaluated. The results demonstrated that A. brasilense had a better growth capacity and IAA production (105.7 ± 10.3 μg ml −1 ) when cultured under biogas composed of 25% CO 2 + 75% methane (CH 4 ) with respect to the control (72.4 ± 7.9 μg ml −1 ), although the ipdC gene expression level was low under the stressful condition generated by biogas.Moreover, this bacterium was able to induce a higher cell density and CO 2 fixation rate from biogas by C. vulgaris (0.27 ± 0.08 g l −1 d −1 ) and S. obliquus (0.22 ± 0.08 g l −1 d −1 ).Conclusions: This study demonstrated that A. brasilense has the capacity to grow and actively maintain its main microalgal growth-promoting mechanism when cultured under biogas and positively influence CO 2 fixation from the biogas of C. vulgaris and S. obliquus. Significance and Impact of the Study:These findings broaden research in the field of Azospirillum-microalga interactions and the prevalence of Azospirillum in environmental and ecological topics in addition to supporting the uses of plant growthpromoting bacteria to enhance biotechnological strategies for biogas upgrading.

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Sulfide removal from livestock biogas by Azospirillum-like anaerobic phototrophic bacteria consortium

Cited by 18 publications

References 10 publications

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Upgrading strategies for effective utilization of biogas

Active indole-3-acetic acid biosynthesis by the bacterium Azospirillum brasilense cultured under a biogas atmosphere enables its beneficial association with microalgae

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