Sulfidogenesis under extremely haloalkaline conditions by Desulfonatronospira thiodismutans gen. nov., sp. nov., and Desulfonatronospira delicata sp. nov. - a novel lineage of Deltaproteobacteria from hypersaline soda lakes

Sorokin, Dimitry Y.; Tourova, T. P.; Henstra, Anne M.; Stams, A.J.M.; Galinski, Erwin A.; Muyzer, Gerard

doi:10.1099/mic.0.2007/015628-0

Cited by 94 publications

(60 citation statements)

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“…3). The SRB partner at the lowest salinity was identified as a member of the genus Desulfonatronum and the SRB partner at the highest salinity was identified as a novel representative of the extremely natronophilic genus Desulfonatronospira (Sorokin et al, 2008). A more specific analysis of the functional gene dsrB showed the presence of a novel Desulfonatronospira in moderate-and high-salt cultures, and confirmed the presence of Desulfonatronum in the low-salt culture (Fig.…”

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confidence: 68%

“…However, only sulfur reduction was apparently active with acetate as electron donor in a relatively short-term incubation experiment. This was corroborated by the results of a cultivation approach, which demonstrated a domination of lithotrophic sulfate-reducing bacteria (SRB) using H 2 /formate, lactate and ethanol as electron donors and disproportionating thiosulfate/sulfite, such as in the genera Desulfonatronum, Desulfonatronovibrio and Desulfonatronospira (Pikuta et al, 1998(Pikuta et al, , 2003Zhilina et al, 1997Zhilina et al, , 2005aSorokin et al, 2008Sorokin et al, , 2011b. Heterotrophic SRB isolated from soda lakes are either 'incomplete oxidizers', producing acetate as a final product during oxidation of simple organic compounds (Desulfobotulus alkaliphilus and Desulfobulbus alkaliphilus; Sorokin et al, 2010bSorokin et al, , 2012 or 'complete oxidizers', which cannot use external acetate (Desulfonatronobacter acidivorans; Sorokin et al, 2012).…”

Section: Introductionmentioning

confidence: 83%

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Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes

et al. 2014

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So far, anaerobic sulfate-dependent acetate oxidation at high pH has only been demonstrated for a low-salt-tolerant syntrophic association of a clostridium 'Candidatus Contubernalis alkalaceticum' and its hydrogenotrophic sulfate-reducing partner Desulfonatronum cooperativum. Anaerobic enrichments at pH 10 inoculated with sediments from hypersaline soda lakes of the Kulunda Steppe (Altai, Russia) demonstrated the possibility of sulfate-dependent acetate oxidation at much higher salt concentrations (up to 3.5 M total Na + ). The most salt-tolerant purified cultures contained two major components apparently working in syntrophy. The primary acetate-fermenting component was identified as a member of the order Clostridiales forming, together with 'Ca. Contubernalis alkalaceticum', an independent branch within the family Syntrophomonadaceae. A provisional name, 'Ca. Syntrophonatronum acetioxidans', is suggested for the novel haloalkaliphilic clostridium. Two phylotypes of extremely haloalkaliphilic sulfatereducing bacteria of the genus Desulfonatronospira were identified as sulfate-reducing partners in the acetate-oxidizing cultures under extreme salinity. The dominant phylotype differed from the two species of Desulfonatronospira described so far, whilst a minor component belonged to Desulfonatronum thiodismutans. The results proved that, contrary to previous beliefs, sulfatedependent acetate oxidation is possible, albeit very slowly, in nearly saturated soda brines.

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confidence: 68%

Section: Introductionmentioning

confidence: 83%

Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes

et al. 2014

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“…Desulfonatronospira Sorokin et al 2008Sorokin et al , 1511 VP (Effective Publication: Sorokin et al 2008Sorokin et al , 1451 De.sul.fo.na.tro.no.spi´ra. L. prep.…”

Section: Phenotypic Analysesmentioning

confidence: 97%

The Family Desulfohalobiaceae

Kuever¹

2014

The Prokaryotes

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“…was reported for mesophilic representatives of the deltaproteobacterial genera Desulfovibrio, Desulfocapsa, Desulfonatronum, Desulfonatronospira and Desulfonatronovibrio (Bak & Pfennig, 1987;Finster et al, 1998;Janssen et al, 1996;Pikuta et al, 2003;Sorokin et al, 2008Sorokin et al, , 2011 as well as for two thermophiles, Dissulfuribacter thermophilus and Dissulfurimicrobium hydrothermale (Slobodkin et al, 2013. Beyond the class Deltaproteobacteria, this ability is known for only one species, Thermosulfurimonas dismutans, belonging to the phylum Thermodesulfobacteria .…”

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confidence: 98%

Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent

Slobodkina

Колганова

Копицын

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent T , was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388 T were Gramstain-negative short rods, 0.2-0.4 µm in diameter and 1.0-2.5 µm in length, and motile with flagella. The temperature range for growth was 25-58 C (optimum 50 C), and the pH range for growth was pH 5.0-7.0 (optimum pH 6.0-6.5). Growth of strain SH388T was observed in the presence of NaCl concentrations ranging from 0.5 to 4.0 % (w/v) (optimum 2.0-2.5 %). The strain grew chemolithoautotrophically with molecular hydrogen as electron donor, sodium sulfite as electron acceptor and bicarbonate/CO 2 as a carbon source. It was also able to grow by disproportionation of sulfite and elemental sulfur but not thiosulfate. Sulfate, Fe(III) and nitrate were not used as electron acceptors either with H 2 or organic electron donors. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the class Deltaproteobacteria and was most closely related to Dissulfuribacter thermophilus and Dissulfurimicrobium hydrothermale (91.6 % and 90.4 % sequence similarity). On the basis of its physiological properties and results of phylogenetic analyses, strain SH388 T is considered to represent a novel species of a new genus, for which the name Dissulfurirhabdus thermomarina gen. nov., sp. nov. is proposed. The type strain of the species is SH388 T (=DSM 100025. It is the first thermophilic disproportionator of sulfur compounds isolated from a shallow-sea environment.Chemolithoautotrophic micro-organisms can gain energy from a variety of inorganic compounds serving as electron donors and acceptors. Sulfur dioxide is one of the most typical and abundant volcanic gases. It is highly soluble in water; thus, in aquatic environments, including hydrothermal vents, SO 2 is usually present in the form of sulfite ions. Micro-organisms capable of dissimilatory sulfite reduction are phylogenetically diverse and include all sulfate-reducers as well as many nonsulfate-reducing species. Overall, the ability to use sulfite as an electron acceptor with organic or inorganic electron donors is known for representatives of the bacterial phyla Firmicutes, Proteobacteria, Nitrospirae and Thermodesulfobacteria and for archaea of the phyla Crenarchaeota and Euryarchaeota (Simon & Kroneck, 2013; Slobodkin et al., 1999). Some sulfite-reducers are also capable of sulfite disproportionation. Growth coupled to disproportionation of sulfite

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Sulfidogenesis under extremely haloalkaline conditions by Desulfonatronospira thiodismutans gen. nov., sp. nov., and Desulfonatronospira delicata sp. nov. - a novel lineage of Deltaproteobacteria from hypersaline soda lakes

Abstract: Sulfidogenesis under extremely haloalkaline conditions by Desulfonatronospira thiodismutans gen. nov., sp. nov., and Desulfonatronospira delicata sp. nov. -a novel lineage of Deltaproteobacteria from hypersaline soda lakes

Cited by 94 publications

References 31 publications

Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes

Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes

The Family Desulfohalobiaceae

Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent

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