Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

Abstract

Moroz, O. M., Hnatush, S. O., Tarabas, O. V., Bohoslavets, C. I., Yavorska, G. V., & Borsukevych, B. M. (2018). Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds. Biosystems Diversity, 26(1), 3-10.Due to their high content in natural environments, heavy metals exhibit toxic effects on living organisms, which leads to a decrease in the biological diversity and productivity of ecosystems. In niches with low oxidation reducing potential, sulfate and sulfur reducing bacteria carry out the reducing transformation of oxidized sulfur compounds with the formation of significant amounts of hydrogen sulfide. H 2 S produced by bacteria interacts with metal ions, precipitating them in the form of sulfides. The aim of this work was to investigate the influence of lead, cuprum (II), iron (II) and manganese (II) salts on the production of hydrogen sulfide by bacteria of the Desulfovibrio and Desulfuromonas genera, isolated from Yavorivske Lake, and to evaluate the efficiency of their use for purifying media, enriched with organic compounds, from hydrogen sulfide and heavy metals. The content of heavy metal ions in the water of Yavorivske Lake was determined by the spectrophotometric method. The bacteria were grown for 10 days at 30 °C in the Kravtsov-Sorokin medium under anaerobic conditions. To study the influence of metal ions on bacteria growth and their H 2 S production, cells were incubated with metal salts (0.5-4.0 mM), washed and grown in media with SO 4 2or S 0 . To determine the level of metal ions binding by H 2 S, produced by bacteria, cells were grown in media with metal compounds (0.5-4.0 mM), SO 4 2or S 0 . Biomass was determined by turbidimetric method. In the cultural liquid the content of H 2 S was determined quantitatively by spectrophotometric method, and qualitatively by the presence of metal cations. The content of metal sulfides in the growth medium was determined by weight method. Sulfate and sulfur-reducing bacteria were resistant to 2.0 mM Pb(NO 3 ) 2 , 2.5 mM CuCl 2 , 2.5 mM FeCl 2 × 4H 2 O and 2.0 mM MnCl 2 × 4H 2 O, therefore they are promising for the development of biotechnologies for the purification of water resources contaminated by sulfur and metal compounds. When present in a medium with sulfates or sulfur of 1.0-1.5 mM lead, cuprum (II), iron (II) or manganese (II) ions, they almost completely bind with the H 2 S produced by bacteria in the form of insoluble sulfides, which confirms the negative results of qualitative reactions to their presence in the cultural liquid.

Reduction of sulfate, nitrate and nitrite ions by Desulfovibrio sp. under the influence of ferrum (III) citrate

“…The intensity of the anaerobic respiration of microorganisms in the contaminated ecotopes is determined by the level of their adaptation to unfavorable environmental conditions, in particular, increased content of metal compounds [18,34,35,37,38]. In the technogenic reservoir that arose on the place of Yavoriv sulfur deposit open pit, high concentrations of toxic to living organisms compounds of sulfur, nitrogen and heavy metals were detected [24,36]. The concentrations of Fe(III) were significantly higher than maximum permissible concentrations (MPC) (0.1−0.3 mg/L [14]) at a depths of 50−70 m and reached to 0.28−0.34 mg/L, while the content of Fe(II) at no depth exceeded the MPC [24].…”

“…In the technogenic reservoir that arose on the place of Yavoriv sulfur deposit open pit, high concentrations of toxic to living organisms compounds of sulfur, nitrogen and heavy metals were detected [24,36]. The concentrations of Fe(III) were significantly higher than maximum permissible concentrations (MPC) (0.1−0.3 mg/L [14]) at a depths of 50−70 m and reached to 0.28−0.34 mg/L, while the content of Fe(II) at no depth exceeded the MPC [24]. The influence of Fe(III) at concentrations significantly higher, than in the reservoir, on sulfate-, nitrate-and nitrite-reducing activity of Desulfovibrio genus bacteria, isolated from Yavorivske Lake, was studied.…”

“…These bacteria play an important role in regulating the level of not only compounds of sulfur and carbon, but also of nitrogen and metals in the environment [2,23,33,[38][39][40]. Pollution of media by heavy metals influences on the physiological and biochemical processes which are carried out by bacteria [10,22,24,25,33].…”

Ovocidal action of glutaraldehyde and benzalkonium chloride mixture on Aonchotheca bovis (Nematoda, Capillariidae) embryogenesis

“…Sulfur-reducing bacteria attract the attention of researchers as potential agents for the treatment of wastewater contaminated with sulfur and sulfur compounds, ions of heavy metals and organic compounds. By performing dissimilation recovery of sulfur, sulfur-reducing bacteria oxidize various organic compounds, thus purifying water reservoirs from organic matter (Qiuet et al, 2017;Moroz et al, 2018). These bacteria oxidize various organic substrates using metals of variable valency as electron acceptors and turn them into forms which are non-toxic or less toxic for living organisms (Smith & Gadd, 1993).…”

“…Hydrogen sulfide accumulation efficiency is influenced by the various environmental factors, such as рН, hydrogen sulfide, presence of various donors and acceptors of electrons, concentration of heavy metal ions etc. (An & Picardal, 2015;Moroz et al, 2018). The objective of this work consisted in the evaluation of the effect of abiotic factors of the medium on sulfidogenic activity of bacteria Desulfuromonas sp.…”