Sulfite Pretreatment (SPORL) for Robust Enzymatic Saccharification of Corn Stalks

Abstract

This study demonstrates sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust bioconversion of corn stalks. With only about 3% sodium bisulfite charge on corn stalks and 30 min pretreatment at temperature 180°C, SPORL can achieve near complete cellulose conversion to glucose in 48h enzymatic hydrolysis. Sulfuric acid charge has great effect on SPORL pretreatment. Enzymatic cellulose conversion of substrate increased with the increase in acid charge under the studied conditions. Bisulfite with different counter-ions can be used in the SPORL. Magnesium as counter ion was found to be more effective on enzymatic hydrolysis of substrate than sodium or ammonium.

Correlation between lignin physicochemical properties and inhibition to enzymatic hydrolysis of cellulose

“…These results also supported the hypothesis that the inhibitory effect of lignin to enzymatic hydrolysis of cellulose was mainly caused by the non-productive adsorption of cellulase (at low concentration) on lignin through hydrophobic interactions. The observations support the results that the sulfite pretreated biomass had good enzymatic digestibility (Kumar et al, 2011;Wang et al, 2009;Zhu et al, 2009), because the residual lignin in the sulfite- Figure 5. Effect of hydrophilically modified lignin on enzymatic saccharification of cellulose and correlation between drop in the glucose yield at 48 h of cellulose caused by addition of the modified lignin and contact angle of the modified lignin.…”

Efficient fractionation of corn stover by bisulfite pretreatment for the production of bioethanol and high value products

“…Previous studies have shown that bisulfite pretreatment is an efficient pretreatment, exhibiting great enzymatic cellulose conversion and ethanol production (Tan et al 2013;Tian et al 2011;Wang et al 2011). In the present study, such a process was selected for the CS pretreatment, after which CS was separated into the cellulosic solids and spent liquor.…”

“…It has been found that NaHSO3 dosage and H2SO4 dosage are the important factors affecting the bisulfite pretreatment (Tan et al 2016). Previous studies have shown that 1% acid almost completely removes the xylan in the raw material (Tan et al 2013;Wang et al 2011). Therefore, the effect of NaHSO3 dosage was investigated relative to bisulfite pretreatment of CS.…”

“…Previous work has shown that bisulfite pretreatment is a promising process that can be used in the effective conversion of different lignocellulose polysaccharides into bioethanol as a liquid fuel source (Zhu et al 2009;Tan et al 2013;Liu et al 2017). Wang et al (2011) and Yu et al (2015) showed that the enzymatic hydrolysis of CS for ethanol production can be significantly improved using bisulfite pretreatment. However, to date, there has been a lack of studies exploring the biorefinery of CS using bisulfite pretreatment.…”

Electrochemical sensor for sulfide determinationin food additives

“…Therefore it is urgent to find approaches to efficiently detect food additives. For example sulfite which is very harmful has been extensively applied in different food processing steps such as preservation of fresh fruits, bacterial inhibition and oxidation resistance in the manufacture process of wines such as grape wine and browning prevention of aquatic products [1][2][3][4][5].To change the status of food safety, technologies for detecting food additives have become the key research subject in food safety field [6][7][8][9][10].…”