Sulfolobales

Huber, Harald; Prangishvili, David

doi:10.1007/0-387-30743-5_3

Cited by 40 publications

(26 citation statements)

References 262 publications

(296 reference statements)

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“…By this scheme, an abasic site generated by loss of guanine and replicated by DNA PolB1 would dictate insertion of dA, yielding a G:C to T:A transversion at that position after another round of replication. High temperature promotes loss of A at about 73% the rate of G loss [39a], but Sulfolobus genomes have 32-38 mol% G+C [39b], making A is nearly twice as abundant as G. As a result, non-enzymatic depurination is predicted to remove A about 25% more frequently than G, on average. The sites of A loss are predicted to be converted by DNA PolB1 (and any other A-rule DNA polymerase) into A:T to T:A transversions, yet such transversions are not observed in spontaneous mutation spectra of the dbh -mutant strains (Fig.…”

Section: Discussionmentioning

confidence: 99%

Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature

2012

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The intrinsically thermostable Y-family DNA polymerases of Sulfolobus spp. have revealed detailed three-dimensional structure and catalytic mechanisms of trans-lesion DNA polymerases, yet their functions in maintaining their native genomes remain largely unexplored. To identify functions of the Y-family DNA polymerase Dbh in replicating the Sulfolobus genome under extreme conditions, we disrupted the dbh gene in Sulfolobus acidocaldarius and characterized the resulting mutant strains phenotypically. Disruption of dbh did not cause any obvious growth defect, sensitivity to any of several DNA-damaging agents, or change in overall rate of spontaneous mutation at a well-characterized target gene. Loss of dbh did, however, cause significant changes in the spectrum of spontaneous forward mutation in each of two orthologous target genes of different sequence. Relative to wild-type strains, dbh− constructs exhibited fewer frame-shift and other small insertion-deletion mutations, but exhibited more base-pair substitutions that converted G:C base pairs to T:A base pairs. These changes, which were confirmed to be statistically significant, indicate two distinct activities of the Dbh polymerase in Sulfolobus cells growing under nearly optimal culture conditions (78-80 °C and pH 3). The first activity promotes slipped-strand events within simple repetitive motifs, such as mononucleotide runs or triplet repeats, and the second promotes insertion of C opposite a potentially miscoding form of G, thereby avoiding G:C to T:A transversions.

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Section: Discussionmentioning

confidence: 99%

Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature

2012

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“…Site SPX, with a much lower pH value of 2, is an ideal habitat for Acidithiobacillus over Hydrogenobacter . Archaea in the Sulfolobales , including Acidianus , which dominated at all pools, are thermoacidophilic sulfur‐oxidizing microorganisms that have been found almost exclusively at solfataras (Huber & Prangishvili, 2006), including those in New Guinea (Plumb et al , 2007) and Montserrat (Burton & Norris, 2000).…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity of boron-rich volcanic hot springs of St. Lucia, Lesser Antilles

Stout

Blake

Greenwood

et al. 2009

FEMS Microbiology Ecology

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“…The S. islandicus barrier is commonly attributed (sometimes implicitly) to the fact that the environmental conditions required by Sulfolobus species (Huber and Prangishvili, 2006) occur in geothermal sites that are extremely limited in area and separated by extremely large distances (Fenchel, 2003;Hahn and Pöckl, 2005;Knittel et al, 2005;Cohan, 2006;Ramette and Tiedje, 2007;Pagaling et al, 2009).…”

Section: Genetic Divergence With Distance In Sulfolobus Speciesmentioning

confidence: 99%

Genomic evidence of rapid, global-scale gene flow in a Sulfolobus species

Mao

Grogan

2012

The ISME Journal

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Local populations of Sulfolobus islandicus diverge genetically with geographical separation, and this has been attributed to restricted transfer of propagules imposed by the unfavorable spatial distribution of acidic geothermal habitat. We tested the generality of genetic divergence with distance in Sulfolobus species by analyzing genomes of Sulfolobus acidocaldarius drawn from three populations separated by more than 8000 km. In sharp contrast to S. islandicus, the geographically diverse S. acidocaldarius genomes proved to be nearly identical. We could not link the difference in genome conservation between the two species to a corresponding difference in genome stability or ecological factors affecting propagule dispersal. The results provide the first evidence that genetic isolation of local populations does not result primarily from properties intrinsic to Sulfolobus and the severe discontinuity of its geothermal habitat, but varies with species, and thus may reflect biotic interactions that act after propagule dispersal.

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Sulfolobales

Cited by 40 publications

References 262 publications

Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature

Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature

Microbial diversity of boron-rich volcanic hot springs of St. Lucia, Lesser Antilles

Genomic evidence of rapid, global-scale gene flow in a Sulfolobus species

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