Sulfone-aromatic ligands for thiophilic adsorption chromatography: Purification of human and mouse immunoglobulins

Interaction of pepsin with aromatic amino acids and their derivatives immobilized to Sepharose

“…The structure of the resulting affinity carrier corresponds to the general formula M-O-CH 2 -CH 2 -SO 2 -CH 2 -CH 2 -X-Y [38,49]. Where M is the agarose matrix, X, besides S, can also be O or N and Y is an aromatic or heteroaromatic compound.…”

“…Studies on the use of these affinity carriers were mainly directed to the thiophilic adsorption separation of different antibodies from various sources. It has been shown that the presence of a thio-ether group is important for introducing selectivity for the given group of proteins [38,49]. N-containing aromatic thiophilic carriers were much less selective for antibodies than regular T-gels [28,50].…”

“…Other nucleophilic atoms (O or N) may sometimes substitute a sulfur atom [26,36,37]. Carriers with sulfone-aromatic ligands were found to possess thiophilic properties and are useful in immunoglobulin separation [38][39][40]. A similar mechanism might participate in binding aspartate proteinases to carriers containing aromatic amino acids immobilized to divinyl sulfone-activated Sepharose [3][4][5].…”

Synthesis of substituted terpyridine ligands for use in protein purification

“…The filtrate from the wash was concentrated to give a second crop of precipitate. The solids were combined and dried under high vacuum to yield a light grey crystalline solid (8.29 g), which was purified by flash column chromatography on a short column of alumina gel using Please cite this article in press as: Lin, C. 7.86 (2H, s, H3 0 ,5 0 ), 7.77 (2H, dd, J 5.2, 1.9 Hz, H5,5 00 ). 13 6.2.5.…”

Capture of a monoclonal antibody and prediction of separation conditions using a synthetic multimodal ligand attached on chips and beads

“…Although, the mechanism of action is not yet completely elucidated, contributions for antibody adsorption come from the presence of imidazole heterocycle [13], the sulphur group [3,4,9] and the aromatic ring [14,15]. The sulfonate group is introduced to prevent the adsorption of acidic species that tend to co-adsorb on the resin by electrostatic repulsion, such as albumin.…”