Sulfone derivatives enter the cytoplasm of Candida albicans sessile cells

Staniszewska, Monika; Sobiepanek, Anna; Gizińska, Małgorzata; Peña‐Cabrera, Eduardo; Arroyo-Córdoba, Ismael J.; Kazek, Michalina; Kuryk, Łukasz; Wieczorek, Magdalena; Koronkiewicz, Mirosława; Kobiela, Tomasz; Ochal, Zbigniew

doi:10.1016/j.ejmech.2020.112139

Cited by 18 publications

(11 citation statements)

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“…Furthermore, 5h induced the phosphatidylserine PE translocation and membrane permeability [ 41 ], these were shown using the Annexin V and propidium iodide PI staining assay ( Figure 3 and Figure 4 ). We hypothesized that PE externalization affect subsequently the elevated chitin content ( Figure 5 ) and activity of 5h .…”

Section: Discussionmentioning

confidence: 95%

“…Based on our results and in line with Nielsen and Sörensen [ 39 ], we hypothesized that 5h displays comparable activity to chitinase (EC 3.2.1.14). Since the ability of Congo red CR fluorescent tracker to visualize the fungal cell wall elements was described previously [ 40 , 41 ], we used CR as a diazo compound pertaining to its high affinity to polysaccharides in the 5h -treated C. albicans [ 40 , 41 ]. In line with Shalmy et al [ 40 ] we found good staining result of CR in the 5h -treated C. albicans vs. 5h -treated capsule of C. neoformans which was poorly stained ( Figure 7 vs. Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

“…Finally, the absorbance at 490 and 660 nm was measured with Synergy H4 Hybrid Reader (BioTek Instruments, Winooski, VT, USA) and specific absorbance (SA) was calculated as follows: SA = A 490 − A 660 . Viability of Vero cells was calculated using formula: % viability = (SA Test − SA Blank) / (SA Positive control − SA Blank) × 100, and the cytotoxicity of the compounds: % cytotoxicity = (SA Positive control − SA Test)/(SA Positive control − SA Blank) × 100 [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…4.2.9. Confocal Laser Scanning Microscopy (CLSM) Analyses of the C. albicans and C. neoformans Biofilms Treated with 5h C. albicans SPZ 176 and clinical C. neoformans SPZ 173 cultures were prepared, as previously described [41]. Suspensions were centrifuged at 5000 rpm at 4 • C for 5 min and resuspended in 2 mL of the YNB medium.…”

Section: Cytometric Analysis Of Cell Death Typementioning

confidence: 99%

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The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

et al. 2021

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Our study aimed to characterise the action mode of N-phenacyldibromobenzimidazoles against C. albicans and C. neoformans. Firstly, we selected the non-cytotoxic most active benzimidazoles based on the structure–activity relationships showing that the group of 5,6-dibromobenzimidazole derivatives are less active against C. albicans vs. 4,6-dibromobenzimidazole analogues (5e–f and 5h). The substitution of chlorine atoms to the benzene ring of the N-phenacyl substituent extended the anti-C. albicans action (5e with 2,4-Cl2 or 5f with 3,4-Cl2). The excellent results for N-phenacyldibromobenzimidazole 5h against the C. albicans reference and clinical isolate showed IC50 = 8 µg/mL and %I = 100 ± 3, respectively. Compound 5h was fungicidal against the C. neoformans isolate. Compound 5h at 160–4 µg/mL caused irreversible damage of the fungal cell membrane and accidental cell death (ACD). We reported on chitinolytic activity of 5h, in accordance with the patterns observed for the following substrates: 4-nitrophenyl-N-acetyl-β-d-glucosaminide and 4-nitrophenyl-β-d-N,N′,N″-triacetylchitothiose. Derivative 5h at 16 µg/mL: (1) it affected cell wall by inducing β-d-glucanase, (2) it caused morphological distortions and (3) osmotic instability in the C. albicans biofilm-treated. Compound 5h exerted Candida-dependent inhibition of virulence factors.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cytometric Analysis Of Cell Death Typementioning

confidence: 99%

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The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

et al. 2021

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“…Positive control means MCR-5 or Vero E6 monolayer in EMEM and Blank: EMEM with the tested conc. of benzoxazoles [ 31 ].…”

Section: Figures Schemes and Tablesmentioning

confidence: 99%

In Vitro Anti-Candida Activity and Action Mode of Benzoxazole Derivatives

et al. 2021

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A newly synthetized series of N-phenacyl derivatives of 2-mercaptobenzoxazole, including analogues of 5-bromo- and 5,7-dibromobenzoxazole, were screened against Candida strains and the action mechanism was evaluated. 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(4-bromophenyl)ethanone (5d), 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(2,3,4-trichloro-phenyl)ethanone (5i), 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(2,4,6-trichlorophenyl)ethanone (5k) and 2-[(5-bromo-1,3-benzoxazol-2-yl)sulfanyl]-1-phenylethanone (6a) showed anti-C. albicans SC5314 activity, where 5d displayed MICT = 16 µg/mL (%R = 100) and a weak anti-proliferative activity against the clinical strains: C. albicans resistant to azoles (Itr and Flu) and C. glabrata. Derivatives 5k and 6a displayed MICP = 16 µg/mL and %R = 64.2 ± 10.6, %R = 88.0 ± 9.7, respectively, against the C. albicans isolate. Derivative 5i was the most active against C. glabrata (%R = 53.0 ± 3.5 at 16 µg/mL). Benzoxazoles displayed no MIC against C. glabrata. Benzoxazoles showed a pleiotropic action mode: (1) the total sterols content was perturbed; (2) 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(3,4-dichlorophenyl)ethanol and 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(2,3,4-trichlorophenyl)ethanol (8h–i) at the lowest fungistatic conc. inhibited the efflux of the Rho123 tracker during the membrane transport process; (3) mitochondrial respiration was affected/inhibited by the benzoxazoles: 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(4-chlorophenyl)ethanol and 2-(1,3-benzoxazol-2-ylsulfanyl)-1-(4-bromophenyl)ethanol 8c–d and 8i. Benzoxazoles showed comparable activity to commercially available azoles due to (1) the interaction with exogenous ergosterol, (2) endogenous ergosterol synthesis blocking as well as (3) membrane permeabilizing properties typical of AmB. Benzoxazoles display a broad spectrum of anti-Candida activity and action mode towards the membrane without cross-resistance with AmB; furthermore, they are safe to mammals.

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Monitoring the Adhesion and Inhibitory Activity of Candida albicans on Poly‐l‐Lysine Modified Gold Nano‐Match Head Arrays

Zhou

et al. 2022

Adv Materials Inter

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found in the interface of implants leading to infection, [2] the surface of ship leading to rust, and the inner wall of underground pipelines leading to block, [3] etc. These microbial biofilms would result in significant financial losses when underground pipelines are corroded by the microorganism, or harm to people's health when implanted medical devices are contaminated by bacteria. So it is critical to prevent the adhesion and afterward formation of biofilm on these material's surfaces. Usually, biofilm formation consists of adhesion, micro-colony formation, architecture formation, and dispersion of the biofilm. [2b] Among these steps, the first step of microbial adhesion is the most critical stage, which initiates the infection and formation of biofilm. This step is influenced by a variety of factors such as surface material, surface electrostatic charge, and environmental factors (e.g., the pH of solution). Thus, it is important to develop a method to in situ evaluate the adhesion and afterward bioactivity of microorganisms on material's surfaces.At present, methods for in situ evaluating the microbial adhesion on material surfaces include fluorescence staining, [4] photonic crystal sensors, [5] electrochemistry, [6] and quartz crystal microbalance (QCM) [7] (Table S1, Supporting Information). The downside of fluorescence staining is not real time. The disadvantage of photonic crystal sensors is that they are insufficiently sensitive. The current generated by QCM and electrochemical impedance in the sensing process will inevitably affect the adhesion of microorganisms, and cause interference to the evaluation of microorganisms adhesion. [8] In addition, monitoring the microbial adhesion onto the material surface and inhibiting microbial activities are two separate processes at present. Most researches only focus on how to monitor the adhesion situation of microorganisms or how to inhibit the activity to kill microorganisms. [9] Therefore, real time, labelfree methods for monitoring the adhesion and inhibitory bioactivity of microorganisms in situ are in great need for the study of microbial adhesion on material surfaces.As we know, localized surface plasmon resonance (LSPR) sensors are sensitive to the refractive index (RI) change near the sensor surface, and therefore are widely used in the A platform for real-time monitoring and comprehensively studying the adhesion process of microorganisms onto material surfaces as well as their bioactivity on the surface is very helpful to reveal the microbial contamination mechanism on drainage catheters, intravascular stents, and implanted sensors. Herein, a multifunctional sensor based on localized surface plasmon resonance of gold nano-match head arrays (GNMAs) is developed for monitoring the adhesion behavior of microorganisms (such as Candida albicans, i.e., C. albicans) as well as its inhibitory activity. The sensor is fabricated through soft lithography processes using nanopatterned polydimethylsiloxane stamps, and modified with polymers of different charg...

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Sulfone derivatives enter the cytoplasm of Candida albicans sessile cells

Cited by 18 publications

References 35 publications

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

In Vitro Anti-Candida Activity and Action Mode of Benzoxazole Derivatives

Monitoring the Adhesion and Inhibitory Activity of Candida albicans on Poly‐l‐Lysine Modified Gold Nano‐Match Head Arrays

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