Sulfonyl-semicarbazide group as a recognition site: A novel ratiometric fluorescent probe for hypochlorous acid and imaging in living cells

Phosphorescent and Test Strips Detection Properties of ClO− Probes Based on Iridium(III) Complexes with Pivaloyl Unit as Recognition Site

“…Nowadays, a variety of recognition moiety sites for HClO/ClO À sensing have been introduced in various probes via different sensing mechanisms. [28] However, most of the reported probes have some shortcomings, such as low fluorescence quantum yield, narrow linear range, poor photostability and interferences from other reactive substances. [29] In general, transition metal complex-based probes, such as cyclometalated Ir(III) complexes, can overcome the above shortcomings due to their favorable photophysical properties including: i) large Stokes shift, which can minimize the possibility of self-quenching; ii) high light stability, which can monitor the target in real time without photobleaching; iii) tunability of π-conjugated ligands, which endow them twophoton absorption behavior, and to be used for two-photon (TP) bioimaging; iv) long luminescence lifetime, that can be used for lifetime imaging and even time-gated luminescence imaging; v) low cytotoxicity and good application in biological samples; vi) abundant electrochemical properties, which suggest them being used in ECL detection.…”

“…As a key element for luminescence probe, the recognition part that can selective and sensitive detection of HClO/ClO − is essential. Nowadays, a variety of recognition moiety sites for HClO/ClO − sensing have been introduced in various probes via different sensing mechanisms [28] . However, most of the reported probes have some shortcomings, such as low fluorescence quantum yield, narrow linear range, poor photostability and interferences from other reactive substances [29] …”