Sulfonylurea resistance in Amaranthus hybridus from southern Brazil

Mendes, Rafael Romero; Silva, Vanessa Francieli Vital; Ferreira, Luiz Augusto Inojosa; Oliveira, Rubem Silvério de

doi:10.1590/0034-737x202269030016

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…1) were generated as commonly described in the literature (Burgos et al, 2013;Takano et al, 2016;Takano et al, 2017;Zobiole et al, 2019;Albrecht et al, 2020b;Albrecht et al, 2020c). Resistance to ALS-inhibiting herbicides in A. hybridus has been observed in neighboring Paraguay, like Brazil, where there are cases of A. hybridus resistance to glyphosate (Resende et al, 2022), multiple resistance to glyphosate and chlorimuron (Heap, 2023), and resistance to chlorimuron and metsulfuron (Mendes et al, 2022). In Argentina, multiple resistance to glyphosate and ALS inhibitors has been highlighted (García et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Amaranthus hybridus L. RESISTANT TO GLYPHOSATE AND CHLORIMURON IN PARAGUAY

Furlan Kashivaqui,

Krzyzaniak,

Pires

et al. 2023

Chil. j. agric. anim. sci.

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Amaranthus species are extremely problematic weeds due to their aggressiveness and resistance, being Amaranthus palmeri S. Watson, Amaranthus retroflexus L., Amaranthus tuberculatus (Moq.) J. D. Sauer, and Amaranthus hybridus L. prominent worldwide. The present research aimed to study A. hybridus populations in Paraguay where the use of glyphosate and chlorimuron herbicides presented management challenges. This research was conducted in the municipalities of Corpus Christi and Hernandarias, Paraguay, in 2016, 2017, 2018, 2019, and 2020. Weed seeds with resistance indicators were collected, cultivated, and following a screening process, cultivated for seed production (heritability up to F2), and the seeds were collected to obtain the herbicide dose-response curve. The herbicides glyphosate and chlorimuron were tested in portions of 1-8, 1-4, 1-2, 1, 2, 4, and 8 times the recommended dose on the package inserts (720 g acid equivalent (ae) ha 1 and 20 g active ingredient (ai) ha1, respectively). Among the A. hybridus populations evaluated in Paraguay, biotype from Hernandarias with multiple resistance to ALS-inhibiting (HRAC 2) and EPSPs-inhibiting (HRAC 9) herbicides, and biotype from Corpus Christi with resistance to ALS-inhibiting herbicides (HRAC 2) were found. Therefore, rotation and combination of herbicides, as well as integration with non-chemical measures, are essential to control and prevent selection of resistant biotypes. Studies aimed to develop integrated weed management plans are essential for effective control. In countries like Paraguay, this becomes imperative because of the lack of research on this topic.

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Section: Resultsmentioning

confidence: 99%

Amaranthus hybridus L. RESISTANT TO GLYPHOSATE AND CHLORIMURON IN PARAGUAY

Furlan Kashivaqui,

Krzyzaniak,

Pires

et al. 2023

Chil. j. agric. anim. sci.

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“…Recently, cross-resistance to imazethapyr and chlorimuron-ethyl was confirmed in Argentina to A. hybridus [31]. Another study in Brazil found cross-resistance to ALS-inhibiting herbicides in an A. hybridus population, with resistance factors of 6.9 and 6.5 for chlorimuron and metsulfuron-ethyl, respectively, but resistance to imazethapyr was not verified [49].…”

Section: Plant Dose-response Curvementioning

confidence: 99%

Absorption, Translocation, and Metabolism of Glyphosate and Imazethapyr in Smooth Pigweed with Multiple Resistance

et al. 2023

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The evolution of herbicide-resistant weeds is the major challenge for chemical management worldwide, increasing production costs, and reducing yield. This work aimed to evaluate the putative resistance of the Amaranthus hybridus population from Candido Mota (CMT) to glyphosate and imazethapyr and to investigate the non-target site mechanisms involved. Dose–response studies were conducted under greenhouse conditions and the control and biomass reduction were evaluated 28 days after application (DAA). Absorption, translocation, and metabolization studies were evaluated at 72 h after treatment (HAT) using radiometric techniques. The dose–response results show different responses among populations to glyphosate and imazethapyr. The CMT population was not controlled with labeled herbicide doses. Based on biomass reduction, the resistance factor was 16.4 and 9.4 to glyphosate and imazethapyr, respectively. The CMT absorbed 66% of 14C-glyphosate and 23% of 14C-imazethapyr at 72 HAT. Although the CMT population absorbed more glyphosate than the susceptible population (12.6%), translocation was impaired for both herbicides in the CMT when compared to the SUS population. There was no evidence that herbicide metabolization was involved in CMT resistance to the herbicides studied. Understanding the mechanisms endowing resistance allows better decision-making. This is the first study that describes non-target-site resistance mechanisms in an Amaranthus hybridus population from Brazil.

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“…Currently, A. hybridus populations have been identified with resistance to inhibitors of photosystem II, acetolactate synthase (ALS), protoporphyrinogen oxidase, 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), and to the synthetic auxin herbicides (Heap 2023). Amaranthus hybridus biotypes from Brazil were identified with resistance to the EPSPS inhibitor, glyphosate, and to ALS-inhibiting herbicides (Mendes et al 2022; Resende et al 2020). However, the mechanism of resistance to ALS-inhibiting herbicides in biotypes from Brazil has not been characterized.…”

Section: Introductionmentioning

confidence: 99%

“…Amaranthus hybridus biotypes from Brazil were identified with resistance to the EPSPS inhibitor, glyphosate, and to ALSinhibiting herbicides (Mendes et al 2022;Resende et al 2020). However, the mechanism of resistance to ALS-inhibiting herbicides in biotypes from Brazil has not been characterized.…”

Section: Introductionmentioning

confidence: 99%

Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

Sulzbach,

Turra,

Cutti

et al. 2023

Weed Sci

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The presence of glyphosate-resistant smooth pigweed (Amaranthus hybridus L.) biotypes has increased in southern Brazil in the last years, presenting the triple amino acid substitution TAP-IVS in the 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), as previously found in Argentina. Some of these biotypes have morphological characteristics of smooth pigweed and redroot amaranth (Amaranthus retroflexus L.). The present study aimed to identify, through molecular markers, the herbicide-resistant species of Amaranthus from Brazil that have the TAP-IVS substitution and to analyze the occurrence of pollen-mediated gene flow (PMGF) as the source of TAP-IVS substitution in these biotypes. Six biotypes were evaluated by the internal transcribed spacer (ITS) sequences, of which two (AMACHY-S and CAMAQ-R) were molecularly classified as A. hybridus, and four (AMACRET-S, AMACVI-S, ARRGR-R, and SAOJER-R) were unclassified. Interestingly, all the glyphosate-resistant biotypes (ARRGR-R, SAOJER-R, and CAMAQ-R) had the TAP-IVS substitution, and an increase in EPSPS relative copy number; however, only CAMAQ-R was confirmed as A. hybridus. Although the biotypes ARRGR-R and SAOJER-R are closely related to A. hybridus and green pigweed (Amaranthus powellii S. Watson), their species identity could not be resolved. The biotype SAOJER-R also was resistant to ALS-inhibiting herbicides due to a tryptophan to leucine substitution at position 574 in acetolactate synthase (ALS). The evaluation of 119,746 seedlings in an intraspecific hybridization study of A. hybridus indicated an outcrossing frequency of 0.09%. In contrast, an absence of interspecific hybridization (A. hybridus × unclassified biotype – AMACVI-S) was found after screening 111,429 offspring. Unclassified biotypes might be derived from one or more ancient hybridization events and subsequently evolved the triple mutation independently. Alternatively, such biotypes could have evolved from recent hybridization events, which occur at a frequency below the level of detection in our study.

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Sulfonylurea resistance in Amaranthus hybridus from southern Brazil

Cited by 4 publications

References 29 publications

Amaranthus hybridus L. RESISTANT TO GLYPHOSATE AND CHLORIMURON IN PARAGUAY

Amaranthus hybridus L. RESISTANT TO GLYPHOSATE AND CHLORIMURON IN PARAGUAY

Absorption, Translocation, and Metabolism of Glyphosate and Imazethapyr in Smooth Pigweed with Multiple Resistance

Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

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