Sulforaphane from broccoli attenuates inflammatory hepcidin by reducing IL-6 secretion in human HepG2 cells

Al-Bakheit, Ala’a; Abu-Qatouseh, Luay

doi:10.1016/j.jff.2020.104210

Cited by 19 publications

(8 citation statements)

References 55 publications

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“…The polyphenol curcumin was also shown to reduce pro-inflammatory cytokine production and directly decrease hepcidin transcription in vitro and in vivo [352,353]. Finally, the Nrf2 activator sulforaphane (SFN), which is a broccoli-derived isothiocyanate (ITC) was shown to counteract the inflammatory induced hepcidin expression with consequent repression of Fpn1 expression in murine macrophages and human cancer cells [146,354].…”

Section: Nutrition In the Treatment Of Anemia Of Inflammationmentioning

confidence: 99%

Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis—Mechanistic Insights into Anemia of Inflammation and Its Treatment

et al. 2021

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Anemia is very common in patients with inflammatory disorders. Its prevalence is associated with severity of the underlying disease, and it negatively affects quality of life and cardio-vascular performance of patients. Anemia of inflammation (AI) is caused by disturbances of iron metabolism resulting in iron retention within macrophages, a reduced erythrocyte half-life, and cytokine mediated inhibition of erythropoietin function and erythroid progenitor cell differentiation. AI is mostly mild to moderate, normochromic and normocytic, and characterized by low circulating iron, but normal and increased levels of the storage protein ferritin and the iron hormone hepcidin. The primary therapeutic approach for AI is treatment of the underlying inflammatory disease which mostly results in normalization of hemoglobin levels over time unless other pathologies such as vitamin deficiencies, true iron deficiency on the basis of bleeding episodes, or renal insufficiency are present. If the underlying disease and/or anemia are not resolved, iron supplementation therapy and/or treatment with erythropoietin stimulating agents may be considered whereas blood transfusions are an emergency treatment for life-threatening anemia. New treatments with hepcidin-modifying strategies and stabilizers of hypoxia inducible factors emerge but their therapeutic efficacy for treatment of AI in ill patients needs to be evaluated in clinical trials.

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Section: Nutrition In the Treatment Of Anemia Of Inflammationmentioning

confidence: 99%

Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis—Mechanistic Insights into Anemia of Inflammation and Its Treatment

et al. 2021

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“…Sulforaphane exposure led to reduction in cell growth, by significantly downregulating key cell cycle-related genes in HepG2 and Huh7 cell lines, and also within a xenograft mouse model ( Sato et al, 2018 ). Sulforaphane decreased secretion of the pro-inflammatory cytokine, interleukin-6, and was not linked with cellular toxicity in HepG2 cells ( Al-Bakheit and Abu-Qatouseh, 2020 ), suggesting that sulforaphane could have anti-inflammatory properties and may modulate liver carcinogenesis.…”

Section: Glucosinolates From Cruciferous Vegetables and Human Healthmentioning

confidence: 99%

Glucosinolates From Cruciferous Vegetables and Their Potential Role in Chronic Disease: Investigating the Preclinical and Clinical Evidence

et al. 2021

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An increasing body of evidence highlights the strong potential for a diet rich in fruit and vegetables to delay, and often prevent, the onset of chronic diseases, including cardiometabolic, neurological, and musculoskeletal conditions, and certain cancers. A possible protective component, glucosinolates, which are phytochemicals found almost exclusively in cruciferous vegetables, have been identified from preclinical and clinical studies. Current research suggests that glucosinolates (and isothiocyanates) act via several mechanisms, ultimately exhibiting anti-inflammatory, antioxidant, and chemo-protective effects. This review summarizes the current knowledge surrounding cruciferous vegetables and their glucosinolates in relation to the specified health conditions. Although there is evidence that consumption of a high glucosinolate diet is linked with reduced incidence of chronic diseases, future large-scale placebo-controlled human trials including standardized glucosinolate supplements are needed.

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“…Since an increase in oxidative stress is always accompanied by an inflammatory process, it was interesting to study the immunomodulatory capacity of these two hempseed peptides in the same cellular system. Notably, HepG2 cells have been widely used as a model for characterizing the anti-inflammatory activity of many food-active compounds. − To achieve this objective, we decided to perform tests under the same conditions and study the same concentration of each peptide H2 (100 μM) and H3 (25 μM), which was previously demonstrated to be safe from a cytotoxic point of view and also effective for antioxidant activity . In particular, HepG2 cells were stimulated with LPS, a generic and commonly used pro-inflammatory stimulus.…”

Section: Discussionmentioning

confidence: 99%

Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

Cruz‐Chamorro

Santos-Sánchez

Bollati

et al. 2022

J. Agric. Food Chem.

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WVSPLAGRT (H2) and IGFLIIWV (H3) are two transepithelial transported intestinal peptides obtained from the hydrolysis of hempseed protein with pepsin, which exert antioxidant activity in HepG2 cells. Notably, both peptides reduce the H 2 O 2 -induced reactive oxygen species, lipid peroxidation, and nitric oxide (NO) production levels in HepG2 cells via the modulation of the nuclear factor erythroid 2-related factor 2 and the inducible nitric oxide synthase (iNOS) pathways, respectively. Due to the close link between inflammation and oxidative stress and with the objective of fostering the multifunctional behavior of bioactive peptides, in this study, the molecular characterization of the anti-inflammatory and immunomodulatory properties of H2 and H3 was carried out in HepG2 cells. In fact, both peptides were shown to modulate the production of pro (IFN-γ: −33.0 ± 6.7% H2, p = 0.011; −13.1 ± 2.0% H3, p = <0.0001; TNF: −17.6 ± 1.7% H2, p = 0.0004; −20.3 ± 1.7% H3, p = <0.0001; and IL-6: −15.1 ± 6.5% H3, p = 0.010)- and anti (IL-10: +9.6 ± 3.1% H2, p = 0.010; +26.0 ± 2.3% H3, p = < 0.0001)-inflammatory cytokines and NO (−9.0 ± 0.7% H2, p = <0.0001; −7.2 ± 1.8% H3, p = <0.0001) through regulation of the NF-κB and iNOS pathways, respectively, in HepG2 cells stimulated by lipopolysaccharides.

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Sulforaphane from broccoli attenuates inflammatory hepcidin by reducing IL-6 secretion in human HepG2 cells

Cited by 19 publications

References 55 publications

Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis—Mechanistic Insights into Anemia of Inflammation and Its Treatment

Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis—Mechanistic Insights into Anemia of Inflammation and Its Treatment

Glucosinolates From Cruciferous Vegetables and Their Potential Role in Chronic Disease: Investigating the Preclinical and Clinical Evidence

Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

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