Sulforaphane inhibited tumor necrosis factor-α induced migration and invasion in estrogen receptor negative human breast cancer cells

Bao, Cheng; Ko, Jiwon; Park, Hyun‐Chang; Kim, Min Chae; Kim, Jongkee; Auh, Joong-Hyuck; Lee, Hong Jin

doi:10.1007/s10068-015-0046-7

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…Organic isothiocyanates (ITCs, 2 , Scheme 1) are a well-studied class of compounds with an array of documented biological applications, including anti-inflammatory [1,2,3,4,5], antioxidant [6,7,8,9], and antitumor effects [10,11,12,13,14]. Of note, consumption of many Brassica vegetables (e.g.…”

Section: Introductionmentioning

confidence: 99%

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

Klingaman

Wagner

Brown

et al. 2017

Analytical Biochemistry

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Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for their beneficial effects in humans. Myrosinase has been shown to be tolerant of non-natural glucosinolates, such as 2,2-diphenylethyl glucosinolate, and can facilitate their conversion to non-natural ITCs, some of which are leads for drug development. An HPLC-based method capable of analyzing this transformation for non-natural systems has been described. This current study describes (1) the Michaelis–Menten characterization of 2,2-diphenyethyl glucosinolate and (2) a parallel evaluation of this analogue and the natural analogue glucotropaeolin to evaluate effects of pH and temperature on rates of hydrolysis and product(s) formed. Methods described in this study provide the ability to simultaneously and independently analyze the kinetics of multiple reaction components. An unintended outcome of this work was the development of a modified Lambert W(x) which includes a parameter to account for the thermal denaturation of enzyme. The results of this study demonstrate that the action of Sinapis alba myrosinase on natural and non-natural glucosinolates is consistent under the explored range of experimental conditions and in relation to previous accounts.

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Section: Introductionmentioning

confidence: 99%

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

Klingaman

Wagner

Brown

et al. 2017

Analytical Biochemistry

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“… 205 SFN prevented TNF-α mediated migration and invasion in breast cancer cells and suppressed MMP-2, -9 and -13 at both transcriptional and translational levels. 206 Lee et al (2012) reported before that SFN suppress MMP-9 expression and invasion in MCF-7 cells via inhibition of NF-κB. 207 …”

Section: Phytochemicals Involved In Breast Cancer Chemopreventionmentioning

confidence: 99%

Mechanistic evaluation of phytochemicals in breast cancer remedy: current understanding and future perspectives

et al. 2018

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“…There is growing interest in chemoprevention, which is intended to prevent or suppress diseases through the chronic administration of synthetic or natural bioactive agents. Isothiocyanates (ITCs) have been reported to possess wonderful chemopreventive effects, including anti-inflammatory, antioxidant, and antitumor effects, yet they are naturally stored in an inactive form named glucosinolates in crucifers with significant edible and commercial value such as broccoli, cauliflower, watercress, Brussels sprouts, and cabbage. , Only after activation by an endogenetic β-thioglucosidase enzyme called myrosinase (Myr), these secondary metabolites glucosinolates (Gls) can be converted to ITCs and hydrolysis contributes to not only a special taste but also health benefits to the human body. Studies have suggested that increasing the ingestion of cruciferous vegetables can reduce the incidence of various diseases, , but the key activator Myr enzyme could be easily denatured through wilting in storage and transporting or food processing , and therefore significantly hinders the utilization of ITCs through diet.…”

Section: Introductionmentioning

confidence: 99%

Near Infrared-II Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Dietary Myrosinase Profiling

et al. 2023

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Myrosinase (Myr) is a type of critical βthioglucosidase enzyme activator essential for sustaining many functional foods to perform their health-promoting functions. An accurate and reliable Myr test is meaningful for food quality and dietary nutrition assessments, whereas the currently reported methods do not guarantee specificity and have high reliance on instrumentation, which are not suitable for rapid and onsite Myr screening especially in complex systems from various sources. Herein, we present a unique NIR-II absorption-based photothermal-responsive colorimetric biosensor for anti-interference onsite Myr determination and realization of rapid visualized outputs with the aid of smartphone calculation. Typically, assisted by glucose oxidase (GOx), Myr specifically converts the sinigrin substrate into hydrogen peroxide (H 2 O 2 ) that can oxidize 3,3′,5,5′tetramethylbenzidine (TMB) catalyzed by AuNPs to form a charge transfer complex (CTC) with NIR-II absorption and photothermal characters. Delightfully, such a proposed method is able to determine Myr within a wide range of 0 to 172.5 mU/mL with a detection limit down to 2.96 mU/mL. Moreover, simple, rapid, and real-time visual Myr identification in actual food-sourced samples could also be readily achieved by smartphone readout processing, with the promising advantages of anti-interference, high accuracy, and low cost as well as labor-saving and intelligence engagement, thus providing great feasibility for precise measurement in complex and dynamic dietary sample analysis. Overall, our proposed method presents a novel technology for onsite dietary Myr enzyme profiling, which is promising to be applied in the food industry for nutritional composition profiles, freshness evaluation, and quality assessment.

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Sulforaphane inhibited tumor necrosis factor-α induced migration and invasion in estrogen receptor negative human breast cancer cells

Cited by 5 publications

References 18 publications

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

Mechanistic evaluation of phytochemicals in breast cancer remedy: current understanding and future perspectives

Near Infrared-II Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Dietary Myrosinase Profiling

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