Sulforaphane modulates TGFβ2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling

Liu, Han-Ruo; Xia, Zi-Yao; Wang, Ningli

doi:10.18240/ijo.2020.10.01

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…In our study, we found that MYLK and HIF1A are involved in the regulation of POSTN. MYLK is known as an important marker of fibrosis [ 35 , 36 ]. POSTN has been reported to activate integrin signaling, which initiates MYLK phosphorylation and leads to reorganization of the actin cytoskeleton important for the regulation of vascular permeability and cell migration [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

The integrated transcriptome bioinformatics analysis identifies key genes and cellular components for proliferative diabetic retinopathy

et al. 2022

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Proliferative Diabetic Retinopathy (PDR) is a chronic complication of Diabetes and the main cause of blindness among the world’s working population at present. While there have been many studies on the pathogenesis of PDR, its intrinsic molecular mechanisms have not yet been fully elucidated. In recent years, several studies have employed bulk RNA-sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) to profile differentially expressed genes (DEGs) and cellular components associated with PDR. This study adds to this expanding body of work by identifying PDR’s target genes and cellular components by conducting an integrated transcriptome bioinformatics analysis. This study integrately examined two public bulk RNA-seq datasets(including 11 PDR patients and 7 controls) and one single-cell RNA-seq datasets(including 5 PDR patients) of Fibro (Vascular) Membranes (FVMs) from PDR patients and control. A total of 176 genes were identified as DEGs between PDR patients and control among both bulk RNA-seq datasets. Based on these DEGs, 14 proteins were identified in the protein overlap within the significant ligand-receptor interactions of retinal FVMs and Protein-Protein Interaction (PPI) network, three of which were associated with PDR (CD44, ICAM1, POSTN), and POSTN might act as key ligand. This finding may provide novel gene signatures and therapeutic targets for PDR.

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Section: Discussionmentioning

confidence: 99%

The integrated transcriptome bioinformatics analysis identifies key genes and cellular components for proliferative diabetic retinopathy

et al. 2022

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“…Moreover, several chemical inhibitors of the PI3K/Akt signaling were investigated in vitro and in vivo for prevention of TGFβ-mediated scar formation in eye disorders. Specifically, sulforaphane, a molecule capable of modulating PI3K/Akt activity, inhibited proliferation, migration, and synthesis of the ECM in human conjunctival fibroblast [ 87 ]. Similarly, lithium chloride was shown to abolish myofibroblast transdifferentiation via PI3K/Akt signal in HTFs [ 88 ].…”

Section: Preclinical Studies With Drugs Affecting Tgfβ For Prevention/treatment Of Ocular Fibrosismentioning

confidence: 99%

Understanding Drivers of Ocular Fibrosis: Current and Future Therapeutic Perspectives

Mallone

Costi

Marenco

et al. 2021

IJMS

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Ocular fibrosis leads to severe visual impairment and blindness worldwide, being a major area of unmet need in ophthalmology and medicine. To date, the only available treatments are antimetabolite drugs that have significant potentially blinding side effects, such as tissue damage and infection. There is thus an urgent need to identify novel targets to prevent/treat scarring and postsurgical fibrosis in the eye. In this review, the latest progress in biological mechanisms underlying ocular fibrosis are discussed. We also summarize the current knowledge on preclinical studies based on viral and non-viral gene therapy, as well as chemical inhibitors, for targeting TGFβ or downstream effectors in fibrotic disorders of the eye. Moreover, the role of angiogenetic and biomechanical factors in ocular fibrosis is discussed, focusing on related preclinical treatment approaches. Moreover, we describe available evidence on clinical studies investigating the use of therapies targeting TGFβ-dependent pathways, angiogenetic factors, and biomechanical factors, alone or in combination with other strategies, in ocular tissue fibrosis. Finally, the recent progress in cell-based therapies for treating fibrotic eye disorders is discussed. The increasing knowledge of these disorders in the eye and the promising results from testing of novel targeted therapies could offer viable perspectives for translation into clinical use.

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Herbal glycosides in healthcare

Soto-Blanco¹

2022

Herbal Biomolecules in Healthcare Applications

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Sulforaphane modulates TGFβ2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling

Cited by 5 publications

References 24 publications

The integrated transcriptome bioinformatics analysis identifies key genes and cellular components for proliferative diabetic retinopathy

The integrated transcriptome bioinformatics analysis identifies key genes and cellular components for proliferative diabetic retinopathy

Understanding Drivers of Ocular Fibrosis: Current and Future Therapeutic Perspectives

Herbal glycosides in healthcare

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