. 2009. Effects of a single cycle of tillage on long-term no-till prairie soils. Can. J. Soil Sci. 89: 521Á530. A study was conducted to examine the effect of tillage on soil conditions and crop growth at three long-term (10 yr) no-till sites, one in each of the Brown, Black, and Gray soil zones of Saskatchewan. The four tillage treatments consisted of one cycle of tillage at three levels of intensity: spring cultivation only, fall'spring cultivation, and fall'spring'disc cultivation, all applied to no-till and also a no-till control. Total and particulate soil organic carbon, soil pH, and soil aggregation were not affected by the tillage operations. Tillage decreased the bulk density in the 5-to 10-cm soil depth, but did not affect soil water content (0Á10 cm) or spring soil temperature (0Á5 cm). Tillage decreased stratification of available phosphorus to some extent, but there appeared to be no associated effect on crop P uptake. Tillage did not effect crop production in any of the 3 yr following its imposition, except at one site where, in the first year, apparent tillage-induced nutrient immobilization resulted in lower yields. Overall, the imposition of one cycle of tillage on long-term no-till soils appears to have little effect on soil properties or crop growth.