Sulfur metabolism in bacteria associated with cheese

Weimer, Bart C.; Seefeldt, Kimberly; Dias, Benjamin

doi:10.1007/978-94-017-2027-4_12

Cited by 57 publications

(90 citation statements)

References 77 publications

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“…Guo et al (1993a) deprived Yoshida sarcoma-bearing nude mice of dietary methionine resulting in tumour regression and extended survival of the mice. Other investigators have used the enzyme methioninase in vivo (Tan et al, 1996a); this enzyme from Pseudomonas putida catalyses the conversion of methionine to methanethiol (Weimer et al, 1999). It has been shown to deplete circulating methionine levels in mice and in humans (Lishko et al, 1993;Tan et al, 1996b).…”

Section: Sw620 Skbr3mentioning

confidence: 99%

Antisense inhibition of methylenetetrahydrofolate reductase reduces survival of methionine-dependent tumour lines

et al. 2002

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Transformed cells have been documented to be methionine-dependent, suggesting that inhibition of methionine synthesis might be useful for cancer therapy. Methylenetetrahydrofolate reductase synthesises 5-methyltetrahydrofolate, the methyl donor utilised in methionine synthesis from homocysteine by vitamin B 12 -dependent methionine synthase. We hypothesised that methylenetetrahydrofolate reductase inhibition would affect cell viability through decreased methionine synthesis. Using medium lacking methionine, but containing homocysteine and vitamin B 12 (M-H+), we found that nontransformed human fibroblasts could maintain growth. In contrast, four transformed cell lines (one colon carcinoma, two neuroblastoma and one breast carcinoma) increased proliferation only slightly in the M-H+ medium. To downregulate methylenetetrahydrofolate reductase expression, two phosphorothioate antisense oligonucleotides, EX5 and 677T, were used to target methylenetetrahydrofolate reductase in the colon carcinoma line SW620; 400 nM of each antisense oligonucleotide decreased cell survival by approximately 80% (P50.01) and 70% (P50.0001), respectively, compared to cell survival after the respective control mismatched oligonucleotide. Western blotting and enzyme assays confirmed that methylenetetrahydrofolate reductase expression was decreased. Two neuroblastoma and two breast carcinoma lines also demonstrated decreased survival following EX5 treatment whereas nontransformed human fibroblasts were not affected. This study suggests that methylenetetrahydrofolate reductase may be required for tumour cell survival and that methylenetetrahydrofolate reductase inhibition should be considered for anti-tumour therapy.

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Section: Sw620 Skbr3mentioning

confidence: 99%

Antisense inhibition of methylenetetrahydrofolate reductase reduces survival of methionine-dependent tumour lines

et al. 2002

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“…These characteristics have resulted in the development of many enzyme drugs for a wide range of disorders (1). L-Methionine γ-lyase (MGL; EC 4.4.1.11) is a pyridoxal 5'-phosphate (PLP)-dependent multifunctional enzyme which catalyzes α, γ-elimination and γ-replacement of L-methionine and its derivatives and also α, β-elimination and β-replacement of S-substituted L-cysteines (2)(3)(4). This activity was first found using dried cells of E. coli and Proteus vulgaris (4).…”

Section: Introductionmentioning

confidence: 99%

“…L-Methionine γ-lyase (MGL; EC 4.4.1.11) is a pyridoxal 5'-phosphate (PLP)-dependent multifunctional enzyme which catalyzes α, γ-elimination and γ-replacement of L-methionine and its derivatives and also α, β-elimination and β-replacement of S-substituted L-cysteines (2)(3)(4). This activity was first found using dried cells of E. coli and Proteus vulgaris (4). MGL have been isolated, purified, and characterized from several microorganisms such as Pseudomonas putida, Clostridium sporogenes, Aeromonas sp., Citrobacter intermedius, Citrobacter freundii, Brevibacterium linens, Trichomonas vaginalis, and Porphyromonas gingivalis (3)(4)(5)(6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

“…This activity was first found using dried cells of E. coli and Proteus vulgaris (4). MGL have been isolated, purified, and characterized from several microorganisms such as Pseudomonas putida, Clostridium sporogenes, Aeromonas sp., Citrobacter intermedius, Citrobacter freundii, Brevibacterium linens, Trichomonas vaginalis, and Porphyromonas gingivalis (3)(4)(5)(6)(7)(8)(9)(10). MGL has not been found in yeast and mammals (7,10,11).…”

Section: Introductionmentioning

confidence: 99%

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Production of methionine γ- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene

Kahraman¹,

Aytan²,

Kurt³

2011

BMB Reports

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“…Sulfate-reducing bacteria are one of the major agents responsible for cecal and colonic hydrogen sulfide (3), probably in feces. However, the bacteria responsible for desulfhydration of sulfur amino acids in the large intestine and feces have not yet been defined; however, some industrial and oralmethanethiol and hydrogen-sulfide producers have been identified (5, 12, 15,22,23,25).…”

mentioning

confidence: 99%

Evaluation of Methanethiol and Hydrogen Sulfide Production by Standard Strains of Intestinal Bacteria and Isolates from Pig Feces

Ushida

Oshima

Tanimura

et al. 2001

Bioscience Microflora

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Methanethiol and hydrogen sulfide generated from feces present an environmental issue in the intensive field of animal agriculture. We have identified four strains of clostridia and five others as methanethiol producers from a range of laboratory strains, which may be present in the large intestines of livestock. We have also isolated one Clostridium perfringens from pig feces as a potent producer of methanethiol. Unlike methanethiol from L-methionine, manylaboratory strains produced hydrogen sulfide from L-cystine.

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Sulfur metabolism in bacteria associated with cheese

Cited by 57 publications

References 77 publications

Antisense inhibition of methylenetetrahydrofolate reductase reduces survival of methionine-dependent tumour lines

Antisense inhibition of methylenetetrahydrofolate reductase reduces survival of methionine-dependent tumour lines

Production of methionine γ- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene

Evaluation of Methanethiol and Hydrogen Sulfide Production by Standard Strains of Intestinal Bacteria and Isolates from Pig Feces

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