Sulfur metabolism in Thiobacillus denitrificans

Abstract

The relatively high specific sulfite reductase activity of 25 mU/mg protein was found in extracts from Thiobacillus dentrificans. The absorption spectrum of the partially pruified enzyme was similar to the siroheme containing sulfite reductases from other sources. It is suggested that the T. denitrificans sulfite reductase may function during the oxidation of reduced sulfur compounds.

Assessment of the stoichiometry and efficiency of CO2 fixation coupled to reduced sulfur oxidation

“… k Nap refers to the periplasmic nitrate reductase system. l References: 1, (Justin and Kelly, 1978 ); 2, (Aminuddin, 1980 ); 3, (Kelly, 1999 ); 4, (Beller et al, 2006 ); 5, (Meyer et al, 2007 ); 6, (Hoor, 1981 ); 7, (Rossetti, 2003 ); 8, (Grabovich et al, 2001 ); 9, (Brannan and Caldwell, 1983 ); 10, (Mason et al, 1987 ); 11, (Lu and Kelly, 1988 ); 12, (Wood and Kelly, 1986 ); 13, (Sorokin et al, 2001 ); 14, (Sorokin et al, 2013 ); 15, (Friedrich et al, 2001 ); 16, (Eccleston and Kelly, 1978 ); 17, (Rohwerder and Sand, 2003 ); 18, (Pronk et al, 1990 ); 19, (Hempfling and Vishniac, 1967 ); 20, (Kelly, 1982 ); 21, (Lengeler et al, 2009 ); 22, (Masau et al, 2001 ); 23, (Kamimura et al, 2005 ); 24, (Nakamura et al, 2014 ); 25, (Wood and Kelly, 1991 ); 26, (Kelly and Wood, 2000 ); 27, (Shi et al, 2011 ); 28, (Odintsova et al, 1993 ); 29, (Sorokin, 2002 ); 30, (Sorokin et al, 2006 ); 31, (Banciu et al, 2008 ); 32, (Smith and Kelly, 1988 ); 33, (Peck, 1960 ); 34, (Suzuki, 1974 ); 35, (Trüper, 1982 ); 36, (Loy et al, 2009 ); 37, (Sorokin et al, 2005 ); 38, (Ruby and Jannasch, 1982 ); 39, (Sievert et al, 2007 ); 40, (Schedel et al, 1975 ); 41, (Cai et al, 2014 ); 42, (Bruckner et al, 2013 ); 43, (Grote et al, 2012 ); 44, (Brettar et al, 2006 ); 45, (Sievert et al, 2008 ); 46, (Nelson and Hagen, 1995 ); 47, (Girguis et al, 20...…”

Phylogeny of Dissimilatory Sulfite Reductases Supports an Early Origin of Sulfate Respiration

“…No amplification could be observed with DNA from bacterial and archaeal species that do not derive energy from sulfate reduction ( Fig. 1), demonstrating that this primer pair does not amplify (i) genes encoding assimilatory sulfite reductase, (ii) genes encoding the sulfite reductase from an organism having the capacity to respire sulfite but not sulfate (Shewanella putrefaciens), or (iii) the genes for the reverse sulfite reductases, which show some similarity to DSR with respect to catalytic parameters and subunit composition (31,32). This is also consistent with recent sequence analysis of this enzyme (siroheme sulfite reductase) from the sulfur-oxidizing phototrophic bacterium, Chromatium vinosum (16).…”

“…Members of the redox enzyme superfamily share enzyme properties or gene sequence motifs with the anaerobically expressed sulfite reductase from Salmonella typhimurium (17), the inducible sulfite reductase from Clostridium pasteurianum (13), and the "reverse sulfite reductases" detectable in the phototrophic sulfur bacterium Chromatium vinosum and in the sulfur-oxidizing chemolithotroph Thiobacillus denitrificans (31,32). Thus, all characterized enzymes that catalyze either the oxidative or reductive (dissimilatory or assimilatory) transformation between sulfite and sulfide appear to be related.…”

Molecular Properties of the Dissimilatory Sulfite Reductase from Desulfovibrio Desulfuricans (Essex) and Comparison with the Enzyme from Desulfovibrio Vulgaris (Hildenborough)

“…Sulfite reductase activity. The sulfite reductase activity was determined in the presence of methyl viologen or cytochrome c3 as mediators (Schedel et al, 1975;Pierik and Hagen, 1991;Steuber et al, 1994) using the manometric method by Umbreit et al (1972). In experiments with whole cells, redox mediators were absent.…”