Sulfur starvation reduces phytosiderophores release by iron-deficient barley plants

Astolfi, Stefania; Cesco, Stefano; Zuchi, Sabrina; Neumann, Günter; Roemheld, Volker

doi:10.1111/j.1747-0765.2006.00010.x

Cited by 66 publications

(58 citation statements)

References 19 publications

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“…It has been demonstrated that S deWciency may limit the release of PS and Fe uptake in barley roots, thus leading to a reduced accumulation of Fe in the leaf tissue (AstolW et al 2006a). Furthermore, it has been shown that Fe deWciency caused an increase in 35 SO 4 2¡ uptake rates in maize and barley plants (AstolW et al 2004(AstolW et al , 2006b.…”

Section: Discussionmentioning

confidence: 98%

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Sulphur deprivation limits Fe-deficiency responses in tomato plants

Zuchi

Cesco

Varanini

et al. 2009

Planta

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111

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The aim of this work was to clarify the role of S supply in the development of the response to Fe depletion in Strategy I plants. In S-sufficient plants, Fe-deficiency caused an increase in the Fe(III)-chelate reductase activity, 59Fe uptake rate and ethylene production at root level. This response was associated with increased expression of LeFRO1 [Fe(III)-chelate reductase] and LeIRT1 (Fe2+ transporter) genes. Instead, when S-deficient plants were transferred to a Fe-free solution, no induction of Fe(III)-chelate reductase activity and ethylene production was observed. The same held true for LeFRO1 gene expression, while the increase in 59Fe2+ uptake rate and LeIRT1 gene over-expression were limited. Sulphur deficiency caused a decrease in total sulphur and thiol content; a concomitant increase in 35SO4(2-) uptake rate was observed, this behaviour being particularly evident in Fe-deficient plants. Sulphur deficiency also virtually abolished expression of the nicotianamine synthase gene (LeNAS), independently of the Fe growth conditions. Sulphur deficiency alone also caused a decrease in Fe content in tomato leaves and an increase in root ethylene production; however, these events were not associated with either increased Fe(III)-chelate reductase activity, higher rates of 59Fe uptake or over-expression of either LeFRO1 or LeIRT1 genes. Results show that S deficiency could limit the capacity of tomato plants to cope with Fe-shortage by preventing the induction of the Fe(III)-chelate reductase and limiting the activity and expression of the Fe2+ transporter. Furthermore, the results support the idea that ethylene alone cannot trigger specific Fe-deficiency physiological responses in a Strategy I plant, such as tomato.

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Section: Discussionmentioning

confidence: 98%

“…Interactions between S and Fe nutrition have been shown in Strategy II plants (Kuwajima and Kawai 1997;AstolW et al 2003AstolW et al , 2004AstolW et al , 2006aBouranis et al 2003), indicating that S deWciency could limit Fe-deWciency response through a decrease in the production and release of PS.…”

Section: Introductionmentioning

confidence: 98%

Sulphur deprivation limits Fe-deficiency responses in tomato plants

Zuchi

Cesco

Varanini

et al. 2009

Planta

Self Cite

111

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“…Gene mRNA levels were normalized with respect to the internal control EF1; relative changes in gene expression were calculated on the basis of their expression levels in Fedeficient plants. Bars represent means ± SD of transcript levels on 2 independent experiments with 3 replicates; capital letters refer to statistically significant differences between treatments (t-test, P<0.05) which limited the uptake of exogenous Fe-PS complexes from the external medium (Astolfi et al 2006). …”

Section: Discussionmentioning

confidence: 99%

Micro-analytical, physiological and molecular aspects of Fe acquisition in leaves of Fe-deficient tomato plants re-supplied with natural Fe-complexes in nutrient solution

et al. 2009

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It is well known that in the rhizosphere soluble Fe sources available for plants are mainly a mixture of complexes between the micronutrient and organic ligands such as organic acids and phytosiderophores (PS) released by roots, microbial siderophores as well as fractions of humified organic matter. In the present work, mechanisms of Fe acquisition operating at the leaf level of plants fed with different Fe-complexes were investigated at the micro-analytical, physiological and molecular levels. Fe-deficient tomato plants (Solanum Lycopersicum L., cv. 'Marmande') were fed for 24 h with a solution (pH 7.5) containing 1 A mu M Fe as Fe-PS, Fe-citrate or Fe-WEHS. Thereafter, leaf tissue was used for the visualization of Fe distribution, measurements of Fe content, reduction and uptake, and evaluation of expression of Fe-chelate reductase (LeFRO1), Fe-transporter (LeIRT1) and Ferritin (Ferritin2) genes. Leaf discs isolated from Fe-deficient plants treated for 24 h with Fe-WEHS developed higher rates of translocation, Fe-chelate reduction and Fe-59 uptake as compared to plants supplied with Fe-citrate or Fe-PS. Leaves of plants treated with Fe-WEHS also showed higher transcript levels of LeFRO1, LeIRT1 and Ferritin2 genes with respect to plants fed with the other Fe-sources. Data obtained support the idea that the efficient use of Fe complexed to WEHS-like humic fractions involves, at least in part, also the activation of Fe-acquisition mechanisms operating at the leaf level

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“…This idea is also supported by the higher uptake rate and translocation of 35S recorded in Fe deprived plants. Interestingly, it was shown that the availability of SO/-can affect accumulation (Kuwajima and Kawai 1997) and release (Astolfi et al 2004) of phytosiderophores in Fe-deficient barley roots.…”

Section: Discussionmentioning

confidence: 99%