Sulfur Transformation in Microbially Mediated Pyrite Oxidation by <i>Acidithiobacillus ferrooxidans</i>: Insights From X-ray Photoelectron Spectroscopy-Based Quantitative Depth Profiling

Li, Juan; Lu, Jianjun; Lu, Xiancai; Tu, Bowen; Ouyang, Bingjie; Han, Xiaodong; Wang, Rucheng

doi:10.1080/01490451.2015.1041182

Cited by 33 publications

(9 citation statements)

References 73 publications

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“…Soil was collected from surface layer of soil up to 6–8 inches, placed in sterilized zipper bags and transferred to Soil and Aquatic Microbiology Lab at Soil and Crop Sciences Department of Texas A&M University. For isolation of targeted microorganisms, the soil sample was enriched with 9K medium 25 supplemented with 1% pyrite and incubated at 25 °C for fifteen days in rotary shaker at 120 rpm. About 50 µl of each enrichment was plated on ferrous tryptic soya agar plates 26 and incubated at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Whole genome sequence analysis reveals high genetic variation of newly isolated Acidithiobacillus ferrooxidans IO-2C

Fariq

Blazier

Yasmin

et al. 2019

Sci Rep

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Acidithiobacillus ferrooxidans, a chemolithoautotrophic bacterium, is well known for its mineral oxidizing properties. The current study combines experimental and whole genome sequencing approaches to investigate an iron oxidizing, extreme acidophilic bacterium, A. ferrooxidans isolate (IO-2C) from an acid seep area near Carlos, TX, USA. Strain IO-2C was capable of oxidizing iron i.e. iron sulphate and iron ammonium sulphate yielding shwertmannite and jarosite minerals. Further, the bacterium’s genome was sequenced, assembled and annotated to study its general features, structure and functions. To determine genetic heterogeneity, it was compared with the genomes of other published A. ferrooxidans strains. Pan-genome analysis displayed low gene conservation and significant genetic diversity in A. ferrooxidans species comprising of 6926 protein coding sequences with 23.04% (1596) core genes, 46.13% (3195) unique and 30.82% (2135) accessory genes. Variant analysis showed >75,000 variants, 287 of them with a predicted high impact, in A. ferrooxidans IO-2C genome compared to the reference strain, resulting in abandonment of some important functional key genes. The genome contains numerous functional genes for iron and sulphur metabolism, nitrogen fixation, secondary metabolites, degradation of aromatic compounds, and multidrug and heavy metal resistance. This study demonstrated the bio-oxidation of iron by newly isolated A. ferrooxidans IO-2C under acidic conditions, which was further supported by genomic analysis. Genomic analysis of this strain provided valuable information about the complement of genes responsible for the utilization of iron and tolerance of other metals.

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Section: Methodsmentioning

confidence: 99%

Whole genome sequence analysis reveals high genetic variation of newly isolated Acidithiobacillus ferrooxidans IO-2C

Fariq

Blazier

Yasmin

et al. 2019

Sci Rep

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“…Tested with six pentlandite-bearing sulfide ores, a similar nickel leaching efficiency was achieved at both pH 2.00 and 3.00. Unlike chalcopyrite [28] and pyrite [29], the bioleaching of nickel from pentlandite could be achieved with a lower concentration of Fe 3+ and higher pH value. Most pentlandite-bearing ores are associated with magnesium-bearing silicate gangue mineral such as olivine and serpentine.…”

Section: Implications For Hydrometallurgical Applications In the Nickmentioning

confidence: 99%

Mechanism for the Bio-Oxidation and Decomposition of Pentlandite: Implication for Nickel Bioleaching at Elevated pH

Sun

Wen

et al. 2020

Minerals

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This work investigated the effects of Fe3+, H+ and adsorbed leaching bacteria on the bioleaching of pentlandite. Collectively, an integrated model for the oxidation and decomposition of pentlandite was built to describe the behaviors of different components in a bioleaching system. Proton ions and ferric ions could promote the break and oxidation of Ni-S and Fe-S bonds. The iron-oxidizing microorganisms could regenerate ferric ions and maintain a high Eh value. The sulfur-oxidizing microorganisms showed significant importance in the oxidation of polysulfide and elemental sulfur. The atoms in pentlandite show different modification pathways during the bioleaching process: iron transformed through a (Ni,Fe)9S8 → Fe2+ → Fe3+ → KFe3(SO4)2(OH)6 pathway; nickel experienced a transformation of (Ni,Fe)9S8 → NiS → Ni2+; sulfur modified through the pathway of S2−/S22− → Sn2− → S0 → SO32− → SO42−. During bioleaching, a sulfur-rich layer and jarosite layer formed on the mineral surface, and the rise of pH value accelerated the process. However, no evidence for the inhibition of the layers was shown in the bioleaching of pentlandite at pH 3.00. This study provides a novel method for the extraction of nickel from pentlandite by bioleaching at elevated pH values.

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“…EDS maps of oxygen showed that elements of oxygen mainly exist in oxides but are scarce in background minerals ( Figure 6H ). These element compositions were akin to the product shapes of pyrite oxide mediated by iron-oxidizing bacteria, such as Acidithiobacillus ferrooxidans ( Li J. et al, 2015 ) and Leptospirillum ferrooxidans ( Harneit et al, 2006 ), and further indicated the iron oxidation function of MM125-6. Together, the functions of autotrophic, iron oxidation and production of exopolysaccharides enable us to explain why the ability of strain MM125-6 grows in oligotrophic modified MMJHS medium and how the genus Alcanivorax adheres to and is active on the surface of iron-rich substrates in various deep sea environments.…”

Section: Discussionmentioning

confidence: 93%

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

et al. 2022

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The genus Alcanivorax is common in various marine environments, including in hydrothermal fields. They were previously recognized as obligate hydrocarbonoclastic bacteria, but their potential for autotrophic carbon fixation and Fe(II)-oxidation remains largely elusive. In this study, an in situ enrichment experiment was performed using a hydrothermal massive sulfide slab deployed 300 m away from the Wocan hydrothermal vent. Furthermore, the biofilms on the surface of the slab were used as an inoculum, with hydrothermal massive sulfide powder from the same vent as an energy source, to enrich the potential iron oxidizer in the laboratory. Three dominant bacterial families, Alcanivoraceae, Pseudomonadaceae, and Rhizobiaceae, were enriched in the medium with hydrothermal massive sulfides. Subsequently, strain Alcanivorax sp. MM125-6 was isolated from the enrichment culture. It belongs to the genus Alcanivorax and is closely related to Alcanivorax profundimaris ST75FaO-1T (98.9% sequence similarity) indicated by a phylogenetic analysis based on 16S rRNA gene sequences. Autotrophic growth experiments on strain MM125-6 revealed that the cell concentrations were increased from an initial 7.5 × 105 cells/ml to 3.13 × 108 cells/ml after 10 days, and that the δ13CVPDB in the cell biomass was also increased from 234.25‰ on day 2 to gradually 345.66 ‰ on day 10. The gradient tube incubation showed that bands of iron oxides and cells formed approximately 1 and 1.5 cm, respectively, below the air-agarose medium interface. In addition, the SEM-EDS data demonstrated that it can also secrete acidic exopolysaccharides and adhere to the surface of sulfide minerals to oxidize Fe(II) with NaHCO3 as the sole carbon source, which accelerates hydrothermal massive sulfide dissolution. These results support the conclusion that strain MM125-6 is capable of autotrophic carbon fixation and Fe(II) oxidization chemoautotrophically. This study expands our understanding of the metabolic versatility of the Alcanivorax genus as well as their important role(s) in coupling hydrothermal massive sulfide weathering and iron and carbon cycles in hydrothermal fields.

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Sulfur Transformation in Microbially Mediated Pyrite Oxidation by Acidithiobacillus ferrooxidans: Insights From X-ray Photoelectron Spectroscopy-Based Quantitative Depth Profiling

Cited by 33 publications

References 73 publications

Whole genome sequence analysis reveals high genetic variation of newly isolated Acidithiobacillus ferrooxidans IO-2C

Whole genome sequence analysis reveals high genetic variation of newly isolated Acidithiobacillus ferrooxidans IO-2C

Mechanism for the Bio-Oxidation and Decomposition of Pentlandite: Implication for Nickel Bioleaching at Elevated pH

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

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