Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer

Takai, Ken; Kobayashi, Hideki; Nealson, Kenneth H.; Horikoshi, Koki

doi:10.1099/ijs.0.02506-0

Cited by 92 publications

(51 citation statements)

References 28 publications

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“…From the growth rates determined in this study, the doubling time was calculated to range from 80 to 92 min. The doubling time agreed very closely with that of culturable strain Sulfurihydrogenibium subterraneum in optimal growth conditions, which is known as a hydrogen-or sulfur-oxidizing chemolithoautotroph and which is phylogenetically related to the large sausage-shaped bacteria associated with the sulfurturf microbial mat (20).…”

supporting

confidence: 59%

“…Microscopic observation and fluorescence in situ hybridization have suggested the dominance of a large sausage-shaped bacterium in sulfur-turf microbial mats (5,8,13,22). Phylogenetic analyses have further revealed that the bacterium belongs to the Hydrogenothermus-Sulfurihydrogenibium clade within the phylum Aquificae (20,22). So far, however, the sausageshaped bacterium has not yet been isolated from sulfur-turf microbial mats as a cultivable strain.…”

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confidence: 99%

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In Situ Biomass Production of a Hot Spring Sulfur-Turf Microbial Mat

et al. 2010

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Sulfur-turf microbial mats develop in sulfide-containing hot spring water dominated by chemolithoautotrophic sulfur-oxidizing bacteria. The sulfur-turf mat that developed at a source of hot water (72°C, pH 6.8) exhibited a growth rate of 0.48±0.04 h −1 and biomass production of 4.6±1.0 mg of C h −1 . On a per-cell basis, this biomass production was at least an order of magnitude higher than the CO2 uptake rate calculated for a photosynthetic mat dominated by thermophilic Synechococcus spp. at 70°C. The sulfur-turf-associated microbial community likely contributes to carbon fixation and primary production in this geothermal habitat.

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confidence: 59%

mentioning

confidence: 99%

In Situ Biomass Production of a Hot Spring Sulfur-Turf Microbial Mat

et al. 2010

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“…DGGE bands NAB10, YSa, and YSe amplified by RT-PCR (Fig. 3B and C) exhibited the highest sequence similarity to a strain of Sulfurihydrogenibium subterraneum (46) and clones GANI3, NAK9, and SRI-240 retrieved from sulfide-rich Japanese (55) and Icelandic (40) geothermal springs (Fig. 4).…”

Section: Phylogenetic Analysis Based On 16s Rdnasmentioning

confidence: 99%

“…There have been several reports concerning the presence of phylotypes related to the sulfur turf clones retrieved from terrestrial hot springs (20,32,37,40) and geothermal water (29,33,45). Recently, a strain of Sulfurihydrogenibium subterraneum, a facultatively anaerobic, hydrogen-or sulfur-thiosulfate-oxidizing, thermophilic chemolithoautotroph, was isolated from subsurface hot aquifer water in a Japanese gold mine (46).…”

mentioning

confidence: 99%

Molecular Characterization of Community Structures and Sulfur Metabolism within Microbial Streamers in Japanese Hot Springs

Nakagawa

Fukui

2003

Appl Environ Microbiol

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Community structures of submerged microbial slime streamers (SMSS) in sulfide-containing hot springs at 72 to 80°C at Nakabusa and Yumata, Japan, were investigated by molecular analysis based on the 16S rRNA gene. The SMSS were classified into two consortia; consortium I occurred at lower levels of sulfide in the hot springs (less than 0.1 mM), and consortium II dominated when the sulfide levels were higher (more than 0.1 mM). The dominant cell morphotypes in consortium I were filamentous and small rod-shaped cells. The filamentous cells hybridized with fluorescent oligonucleotide probes for the domain Bacteria, the domain Archaea, and the family Aquificaceae. Our analysis of the denaturing gradient gel electrophoresis (DGGE) bands by using reverse transcription (RT)-PCR amplification with two primer sets (Eub341-F with the GC clamp and Univ907R for the Bacteria and Eub341-F with the GC clamp and Arch915R) indicated that dominant bands were phylogenetically related to microbes in the genus Aquifex. On the other hand, consortium II was dominated by long, small, rod-shaped cells, which hybridized with the oligonucleotide probe S-‫-ء‬Tdes-0830-a-A-20 developed in this study for the majority of as-yet-uncultivated microbes in the class Thermodesulfobacteria. The dominant DGGE band obtained by PCR and RT-PCR was affiliated with the genus Sulfurihydrogenibium. Moreover, our analysis of dissimilatory sulfite reductase (DSR) gene sequences retrieved from both consortia revealed a high frequency of DSR genes corresponding to the DSR of Thermodesulfobacteria-like microorganisms. Using both sulfide monitoring and 35 SO 4 2؊ tracer experiments, we observed microbial sulfide production and consumption by SMSS, suggesting that there is in situ sulfide production by as-yet-uncultivated Thermodesulfobacteria-like microbes and there is in situ sulfide consumption by Sulfurihydrogenibium-like microbes within the SMSS in the Nakabusa and Yumata hot springs.Over the past decade, studies of the microbiology of hightemperature terrestrial hot springs by both molecular-ecological and culture-based approaches have revealed phylogenetic and physiological diversity (20-23, 32, 37, 38, 40, 55). While culture-dependent approaches are effective for physiological characterization of isolated thermophiles, they are not useful for providing comprehensive ecophysiological information about the microbial populations in thermophilic environments. One reason for this is that cultivated microbes from hightemperature habitats may frequently represent minor members of a microbial community whose representation is similar to the representation found in the native setting (52).Sulfate-reducing bacteria (SRB), which obtain energy from dissimilatory sulfate reduction, are widespread in anoxic environments and play an important role in the sulfur cycle (53). Several strains belonging to the genus Thermodesulfobacterium, which includes thermophilic and gram-negative SRB, have been isolated from sediments and filamentous microbial communities in terrestrial...

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“…Persephonella, Hydrogenothermus and Sulfurihydrogenibium are located in the family Hydrogenothermaceae of the order (Eder and Huder, 2002;Takai et al, 2003a). Thermovibrio and Balnearium together with Desulfurobacterium, represent a tentative family Desulfurobacteriaceae of the order Takai et al, 2003b).…”

Section: Aquificalesmentioning

confidence: 99%

Polyamine analysis for chemotaxonomy of thermophilic eubacteria: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales

Hosoya

Hamana

Niitsu

et al. 2004

J. Gen. Appl. Microbiol.

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IntroductionAliphatic cellular polyamines, linear diamines, triamines, tetra-amines, penta-amines and hexa-amines, and quaternary branched penta-amines, were found in eubacteria (domain Bacteria) and archaebacteria (doPolyamine analysis for chemotaxonomy of thermophilic eubacteria:Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales Cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by HPLC and GC analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. Spermidine and a quaternary branched penta-amine, N 4 -bis(aminopropyl)norspermidine, were the major polyamine in Thermocrinis, Hydrogenobacter, Hydrogenobaculum, Aquifex, Persephonella, Sulfurihydrogenibium, Hydrogenothermus, Balnearium and Thermovibrio, located in the order Aquificales. Thermodesulfobacterium and Thermodesulfatator belonging to the order Thermodesulfobacteriales contained another quaternary penta-amine, N 4 -bis(aminopropyl)spermidine. In the order Thermotogales, Thermotoga contained spermidine, norspermidine, caldopentamine and homocaldopentamine. The latter two linear penta-amines were not found in Marinitoga and Petrotoga. In the order Thermales, Thermus and Marinithermus contained homospermidine, norspermine and the linear penta-amines. Meiothermus lacked penta-amines. Vulcanithermus contained linear penta-amines and hexa-amines but not homospermidine. Oceanithermus contained spermine alone. Within the order Thermoanaerobacteriales, the two quaternary branched penta-amines were found in Thermanaeromonas and Thermoanaerobacter. Caldanaerobacter contained N 4 -bis(aminopropyl)spermidine. Thermoanaerobacterium lacked penta-amines. Thermaerobacter of the order Clostridiales contained N 4 -bis(aminopropyl)spermidine and agmatine. Thermosyntropha, Thermanaerovibrio, Thermobrachium (the order Clostridiales), Sulfobacillus, Alicyclobacillus, Anoxybacillus, Ureibacillus, Thermicanus (the order Bacillales), Desulfotomaculum, Desulfitobacterium and Pelotomaculum (the family Peptococcaceae) ubiquitously contained spermine. Some thermophiles of Bacillales added linear and branched penta-amines.

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Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer

Cited by 92 publications

References 28 publications

In Situ Biomass Production of a Hot Spring Sulfur-Turf Microbial Mat

In Situ Biomass Production of a Hot Spring Sulfur-Turf Microbial Mat

Molecular Characterization of Community Structures and Sulfur Metabolism within Microbial Streamers in Japanese Hot Springs

Polyamine analysis for chemotaxonomy of thermophilic eubacteria: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales

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