Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines

Abstract

The studies concerned the expression of sulfurtransferases and cystathionine beta-synthase in six human leukemia cell lines: B cell acute lymphoblastic leukemia-B-ALL (REH cells), T cell acute lymphoblastic leukemia-T-ALL (DND-41 and MOLT-4 cells), acute myeloid leukemia—AML (MV4-11 and MOLM-14 cells), and chronic myeloid leukemia—CML (K562 cells). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed to determine the expression of thiosulfate sulfurtransferase, 3-mercaptopyruvate sulfurtransferase, gamma-cystathionase, and cystathionine beta-synthase on the mRNA and protein level. Interestingly, we found significant differences in the mRNA and protein levels of sulfurtransferases and cystathionine beta-synthase in the studied leukemia cells. The obtained results may contribute to elucidating the significance of the differences between the studied cells in the field of sulfur compound metabolism and finding new promising ways to inhibit the proliferation of various types of leukemic cells by modulating the activity of sulfurtransferases, cystathionine beta-synthase, and, consequently, the change of intracellular level of sulfane sulfur as well as H2S and reactive oxygen species production.

Bridging the Gap in Cancer Research: Sulfur Metabolism of Leukemic Cells with a Focus on L-Cysteine Metabolism and Hydrogen Sulfide-Producing Enzymes

“…The research results find consistency with our previous work by Jurkowska et al [27]. The expression of TST in MOLT-4 was practically absent, which aligns with the data presented in Figure 4.…”

“…Furthermore, our previous research aligns with these findings, as we discovered unchanged rhodanese expression in all examined AML (MOLM-14, MV4 cell lines) and CML (K562 cell line) cells. However, rhodanese was not expressed in T-cell Acute Lymphoblastic Leukemia (T-cell ALL) cell lines (DND-41 and MOLT-4) [27]. The results concerning the MOLT-4 cell line are consistent with the data presented on Figure 4.…”

“…All of these factors underscore the need for research into new therapeutic techniques that can either further extend patients' lives or offer complete recovery. In light of these challenges, our laboratory's recent investigations into specific enzymes in leukemic cell lines have yielded promising results [27]. This paper aims to offer an indepth review of the current literature, focusing specifically on the role of enzymes in sulfur metabolism within leukemic cells.…”

Mutations in Genes Producing Nitric Oxide and Hydrogen Sulfide and Their Connection With Apoptotic Genes in Chronic Myeloid Leukemia

“…Chronic myeloid leukemia (CML), also known as chronic myelogenous leukemia, is a myeloproliferative neoplasm that involves uncontrolled myeloid cell growth [1]. CML differs from other myeloproliferative neoplasms because of the BCR-ABL1 fusion gene and Philadelphia chromosome (Ph) caused by t(9;22) (q34.1;q11.2) [2][3][4].…”

Novel Regulatory Roles of Hydrogen Sulfide in Health and Disease

“…Since the demonstration [ 24 ] that the H 2 S-producing enzyme CBS is overexpressed in colon cancer, and that it plays various cancer-cell-supporting roles (such as cytoprotection, proliferative effects, pro-angiogenic effects and others), this field has steadily expanded (as reviewed recently in [ 10 ]). In the current Special Issue, Wrobel’s group published two articles focusing on the expression patterns of various H 2 S-producing enzymes in a variety of commonly used cell lines, and identified some commonalities as well as important cell-type-differences [ 25 , 26 ]. Moreover, in a study utilizing a patient-derived xenograft model, our own group has demonstrated that those colon cancers that express higher levels of CBS, when implanted onto nude mice, proliferate faster and respond better to pharmacological CBS inhibition than those colon cancers that express low levels of CBS [ 27 ].…”