2003
DOI: 10.1007/s00425-003-1076-6
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SulP , a nuclear gene encoding a putative chloroplast-targeted sulfate permease in Chlamydomonas reinhardtii

Abstract: Genomic, proteomic, phylogenetic and evolutionary aspects of a novel gene encoding a putative chloroplast-targeted sulfate permease of prokaryotic origin in the green alga Chlamydomonas reinhardtii are described. This nuclear-encoded sulfate permease gene (SulP) contains four introns, whereas all other known chloroplast sulfate permease genes lack introns and are encoded by the chloroplast genome. The deduced amino acid sequence of the protein showed an extended N-terminus, which includes a putative chloroplas… Show more

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Cited by 47 publications
(53 citation statements)
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“…Transcripts from some of the transporter genes increase significantly during S deprivation; increased accumulation of SLT1 and SULTR2 transcripts during S starvation was previously reported (Gonzalez-Ballester et al, 2008). Chlamydomonas also possesses SO 4 22 permeases (SulP1 and SulP2) that resemble the permeases of bacteria (Laudenbach and Grossman, 1991;Chen et al, 2003;Lindberg and Melis, 2008). These transport proteins have been localized to the chloroplast envelope and function in the transport of SO 4 22 from the cytosol into chloroplasts, where reductive assimilation of the anion occurs.…”
mentioning
confidence: 90%
“…Transcripts from some of the transporter genes increase significantly during S deprivation; increased accumulation of SLT1 and SULTR2 transcripts during S starvation was previously reported (Gonzalez-Ballester et al, 2008). Chlamydomonas also possesses SO 4 22 permeases (SulP1 and SulP2) that resemble the permeases of bacteria (Laudenbach and Grossman, 1991;Chen et al, 2003;Lindberg and Melis, 2008). These transport proteins have been localized to the chloroplast envelope and function in the transport of SO 4 22 from the cytosol into chloroplasts, where reductive assimilation of the anion occurs.…”
mentioning
confidence: 90%
“…Until recently, however, genes encoding for the sulfate transporters in C. reinhardtii were not identified. In recent work, we reported the identification and characterization of SulP (GenBank Accession numbers AF467891 and AF481828), a novel and unusual nuclear gene encoding a putative chloroplast-targeted sulfate permease (Chen et al 2003). The deduced amino acid sequence of the SulP protein had a substantial homology with the CysT gene product of cyanobacteria and with the chloroplast-encoded sulfate permease in many green algae (Chen et al 2003).…”
Section: Introductionmentioning
confidence: 98%
“…Available evidence supports a role for the SulP gene product in chloroplast sulfate transport in Chlamydomonas as, for example, it is induced by sulfur-deficiency and antisense transformants are impaired in sulfate transport (Chen and Melis 2004;Chen et al 2005). No such genes have been found in vascular plants (Chen et al 2003).…”
Section: Transport Into the Chloroplastmentioning
confidence: 97%
“…The triose-phosphate/phosphate translocator has been cloned (Flügge et al 1989) but no conclusive evidence has been presented that this transporter is responsible for sulfate uptake into the chloroplast in vivo. A multi-subunit ABC-type transporter has been shown to be present in the chloroplast membrane of Chlamydomonas reinhardtii (Chen and Melis 2004;Chen et al 2005;Chen et al 2003;Melis and Chen 2005). It is proposed that the transporter comprises a heterodimer of transmembrane proteins constituting the 'pore', two associated cytosolic sulfate binding proteins and two stroma located ATP binding subunits attached to each of the membrane subunits .…”
Section: Transport Into the Chloroplastmentioning
confidence: 99%