Sulphate and Chloride-Dependent Potassium Transport in Human Erythrocytes are Affected by Crude Venom from Nematocysts of the Jellyfish<b><i>Pelagia noctiluca</i></b>

Morabito, Rossana; Marino, Angela; Romano, P.; Rigano, Caterina; Spada, Giuseppina La

doi:10.1159/000356630

Cited by 20 publications

(21 citation statements)

References 41 publications

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“…7 To prove this latter feature, a variety of assays has been validated so far, with the cytotoxic assay, performed on erythrocytes 18,19 or on cultured cells [20][21][22] chosen as the most suitable for in vitro studies. For the present investigation, human epithelial kidney cultured cells (HEK 293 Phoenix) were used as a model to verify the cytotoxic effect of both S. plicata and A. mentula extracts.…”

Section: Discussionmentioning

confidence: 99%

Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)

et al. 2016

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The biological activity of extract obtained from the ascidians Styela plicata and Ascidia mentula, living in the coastal lake Faro (Messina, Italy) was ascertained with specific regard to cytotoxic effect, tested on cultured human embryonic kidney (HEK 293 Phoenix) cells after 12, 24 or 48 h, and to antimicrobial effect (10 mg/mL), assayed on both Gram positive and negative strains. Nitrosative stress, possibly induced by the extract on HEK 293 cells and assessed by NO2 -/NO3 -production measurement, was also verified. With regard to cytotoxic activity, A. mentula extract was more effective than S. plicata one, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, with toxic action due to nitrosative stress. S. plicata extract had a more significant antimicrobial activity than A. mentula. The present findings provide a first evidence of the biological power exhibited by both S. plicata and A. mentula extracts, thus increasing the knowledge about the biological activity of marine compounds and providing novel information to possibly correlate the different toxicity pattern displayed by both specimens and their distribution in the lake Faro (Messina).

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Section: Discussionmentioning

confidence: 99%

Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)

et al. 2016

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“…It is thus reasonable to consider this parameter to monitor cell function in different conditions, such as diseases, exposure to drugs, pollutants and metabolites, with their possible detrimental effects, including oxidative stress events. [26][27][28][29][30][31] Amongst renal alterations, interesting is the case of renal I/R injury, which causes acute renal failure in several clinical settings like kidney transplantation. 32,33 The mechanisms of renal acute injury induced by I/R is multifactorial and involves hypoperfusion, inflammatory responses, along with free radical induced damage.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress affects responsiveness to hypotonicity of renal cells

et al. 2014

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Oxidative stress plays a critical role in the pathophysiology of several kidney diseases and is the consequence of alterations like ischemic events. The regulatory volume decrease (RVD) is an homeostatic response essential to many cells, including renal cells, to counteract changes in the osmolarity of the external medium. The aim of the present work is to verify whether oxidative stress affects RVD in a model of renal cells (human embryonic kidney cells, HEK 293 Phoenix). To accomplish this aim, the experimental procedure consisted in: i) cell culture preparation and treatment with 200 µM H2O2; and ii) measurement of cell volume changes in isotonic conditions or following hypotonic stress. H2O2 added to the extracellular isotonic solution induced a significant reduction in cell volume, and added to the extracellular hypotonic solution dramatically impaired the expected osmotic cell swelling. Pre-incubation of cells in an extracellular isotonic solution containing H2O2 prevented cell from swelling after hypotonic stress application. In conclusion, H2O2 leads to cell shrinkage in isotonic conditions, inhibits the hypotonicity-induced cell swelling and consequently prevents RVD, hypothetically due to an activation of transport pathways determining ion loss and, in turn, water efflux. Cell shrinkage in isotonic conditions is a hallmark of apoptosis and is known as the apoptotic volume decrease.

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“…-SH groups determination Erythrocytes, after exposure to external medium at different pH values (6.5, 7.4, 8.5) or to 2 mM NEM, with or without curcumin (1 or 10 µM), were addressed to -SH groups determination technique [15,23], after Band 3 protein isolation. For this purpose, erythrocytes were re-suspended to 10% hematocrit in 20 ml pH 7.4 isotonic buffer and then hemolyzed by re-suspension in 20 ml of cold hypotonic buffer (5mM sodium phosphate, pH 7.4).…”

Section: Exposure To Medium At Different Ph Values or To Nemmentioning

confidence: 99%

Curcumin Protects -SH Groups and Sulphate Transport after Oxidative Damage in Human Erythrocytes

Morabito

Falliti

Geraci³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Erythrocytes, continuously exposed to oxygen pressure and toxic compounds, are sensitive to oxidative stress, namely acting on integral Band 3 protein, with consequences on cell membranes deformability and anion transport efficiency. The aim of the present investigation, conducted on human erythrocytes, is to verify whether curcumin (1 or 10µM), a natural compound with proved antioxidant properties, may counteract Band 3-mediated anion transport alterations due to oxidative stress. Methods: Oxidative conditions were induced by exposure to, alternatively, either 2 mM N-ethylmaleimide (NEM) or pH-modified solutions (6.5 and 8.5). Rate constant for SO₄⁼ uptake and -SH groups estimation were measured to verify the effect of oxidative stress on anion transport efficiency and erythrocyte membranes. Results: After the exposure of erythrocytes to, alternatively, NEM or pH-modified solutions, a significant decrease in both rate constant for SO₄⁼ uptake and -SH groups was observed, which was prevented by curcumin, with a dose-dependent effect. Conclusions: Our results show that: i) the decreased efficiency of anion transport may be due to changes in Band 3 protein structure caused by cysteine -SH groups oxidation, especially after exposure to NEM and pH 6.5; ii) 10 µM Curcumin is effective in protecting erythrocytes from oxidative stress events at level of cell membrane transport.

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Sulphate and Chloride-Dependent Potassium Transport in Human Erythrocytes are Affected by Crude Venom from Nematocysts of the JellyfishPelagia noctiluca

Cited by 20 publications

References 41 publications

Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)

Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)

Oxidative stress affects responsiveness to hypotonicity of renal cells

Curcumin Protects -SH Groups and Sulphate Transport after Oxidative Damage in Human Erythrocytes

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