Sulphur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells ofChromatium okenii

Trueper, H. G.; Schlegel, H. G.

doi:10.1007/bf02046728

Cited by 795 publications

(321 citation statements)

References 23 publications

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“…of headspace. Sulfide (HS − ) formation was measured using the standard methylene blue method (Trüper and Schlegel, 1964). The values were obtained by subtraction of the measurements in control (no cells) from the data in the culture.…”

Section: Origin Of Samples Enrichments and Cultivation Conditionsmentioning

confidence: 99%

Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon

et al. 2015

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Archaea domain is comprised of many versatile taxa that often colonize extreme habitats. Here, we report the discovery of strictly anaerobic extremely halophilic euryarchaeon, capable of obtaining energy by dissimilatory reduction of elemental sulfur using acetate as the only electron donor and forming sulfide and CO 2 as the only products. This type of respiration has never been observed in hypersaline anoxic habitats and is the first example of such metabolic capability in the entire Archaea domain. We isolated and cultivated these unusual organisms, selecting one representative strain, HSR2, for detailed characterization. Our studies including physiological tests, genome sequencing, gene expression, metabolomics and [ 14 C]-bicarbonate assimilation assays revealed that HSR2 oxidized acetate completely via the tricarboxylic acid cycle. Anabolic assimilation of acetate occurred via activated glyoxylate bypass and anaplerotic carboxylation. HSR2 possessed sulfurtransferase and an array of membrane-bound polysulfide reductase genes, all of which were expressed during the growth. Our findings suggest the biogeochemical contribution of haloarchaea in hypersaline anoxic environments must be reconsidered.

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Section: Origin Of Samples Enrichments and Cultivation Conditionsmentioning

confidence: 99%

Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon

et al. 2015

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“…An ethanolic solution of d-UQ was then added at a concentration of 200 lM and after creating anaerobic conditions, 100 lM of sulfide was introduced by a syringe. The bottles were incubated up to 30 min with regular sampling of 0.2 mL directly into 1 mL of 2% Zn-acetate to analyze residual sulfide by the methylene-blue method (Trüper and Schlegel 1964). Boiled membrane preparations were used as a biological control.…”

Section: Enzymatic and Chemical Analysesmentioning

confidence: 99%

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

et al. 2008

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“…The inoculation was done directly in the field. Growth was monitored within 4-8 weeks incubation at room temperature by estimating the sulfide production (Trüper and Schlegel 1964). Tubes with the highest positive dilutions were then subjected to DGGE analysis.…”

Section: Quantification Of Sulfate-reducing Bacteria By Serial Dilutionsmentioning

confidence: 99%

Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia)

et al. 2007

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Here we describe the diversity and activity of sulfate reducing bacteria along a salinity gradient in four different soda lakes from the Kulunda Steppe (South East Siberia, Russia). For this purpose, a combination of culture-dependent and independent techniques was applied. The general bacterial and SRB diversity were analyzed by denaturing gradient gel electrophoresis (DGGE) targeting the 16S rDNA gene. DNA was used to detect the microbial populations that were present in the soda lake sediments, whereas ribosomal RNA was used as a template to obtain information on those that were active. Individual DGGE bands were sequenced and a phylogenetic analysis was performed. In addition, the overall activity of SRB was obtained by measuring the sulfate reduction rates (SRR) and their abundance was estimated by serial dilution. Our results showed the presence of minor, but highly active microbial populations, mostly represented by members of the Proteobacteria. Remarkably high SRR were measured at hypersaline conditions (200 g L @1 ). A relatively high viable count indicated that sulfate reducing bacteria could be highly active in hypersaline soda lakes. Furthermore, the increase of sodium carbonate/bicarbonate seemed to affect the composition of the microbial community in soda lakes, but not the rate of sulfate reduction.

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Sulphur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells ofChromatium okenii

Cited by 795 publications

References 23 publications

Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon

Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia)

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