Sulphur Nutrition and Legume Seed Quality

Imsande, John

doi:10.1007/978-94-017-0289-8_16

Cited by 5 publications

(4 citation statements)

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“…This is in accordance with the findings of other studies that revealed the remarkable plasticity of the soybean seed proteome and the adaptive response to insufficient amounts of β-conglycinin. − Furthermore, the content of sulfur-containing amino acids was significantly higher in the seeds of the CF- LincCG1 plants when compared to those of DN50 plants. Consistent with this observation, the content of the sulfur-containing amino acids Met and Cys in glycinin is 3- to 4-fold higher than that in β-conglycinin. , The total sulfur-containing amino acid content is likely to be higher in the β-conglycinin-deficient lines than in the wild-type varieties. − In the current study, the free Arg content was found to be significantly higher in the seeds of the CF- LincCG1 plants when compared to those of the DN50 plants. In fact, the loss of major storage proteins is occasionally compensated by an increase in free amino acid content. − The results obtained herein suggest that the β-conglycinin deficiency arising from the CRISPR/Cas9-mediated knockout of lincCG1 (resulting in the null allele LincCG1 ) does not exert any detrimental effects on the total protein content or amino acid quality of the mature seeds from the mutant lines.…”

Section: Discussionsupporting

confidence: 80%

Generation of New β-Conglycinin-Deficient Soybean Lines by Editing the lincRNA lincCG1 Using the CRISPR/Cas9 System

Song,

Luo,

Fan

et al. 2024

J. Agric. Food Chem.

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Soybean β-conglycinin is a major allergen that adversely affects the nutritional properties of soybean. Soybean deficient in β-conglycinin is associated with low allergenicity and high nutritional value. Long intergenic noncoding RNAs (lincRNAs) regulate gene expression and are considered important regulators of essential biological processes. Despite increasing knowledge of the functions of lincRNAs, relatively little is known about the effects of lincRNAs on the accumulation of soybean βconglycinin. The current study presents the identification of a lincRNA lincCG1 that was mapped to the intergenic noncoding region of the β-conglycinin α-subunit locus. The full-length lincCG1 sequence was cloned and found to regulate the expression of soybean seed storage protein (SSP) genes via both cis-and trans-acting regulatory mechanisms. Loss-of-function lincCG1 mutations generated using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system led to the deficiency of the allergenic α′-, α-, and β-subunits of soybean β-conglycinin as well as higher content of proteins, sulfur-containing amino acids, and free arginine. The dominant null allele LincCG1, and consequently, the β-conglycinin-deficient phenotype associated with the lincCG1-gene-edited line was stably inherited by the progenies in a Mendelian fashion. The dominant null allele LincCG1 may therefore be exploited for engineering/developing novel hypoallergenic soybean varieties. Furthermore, Cas9-free and β-conglycinin-deficient homozygous mutant lines were obtained in the T 1 generation. This study is the first to employ the CRISPR/ Cas9 technology for editing a lincRNA gene associated with the soybean allergenic protein β-conglycinin. Moreover, this study reveals that lincCG1 plays a crucial role in regulating the expression of the β-conglycinin subunit gene cluster, besides highlighting the efficiency of employing the CRISPR/Cas9 system for modulating lincRNAs, and thereby regulating soybean seed components.

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Section: Discussionsupporting

confidence: 80%

Generation of New β-Conglycinin-Deficient Soybean Lines by Editing the lincRNA lincCG1 Using the CRISPR/Cas9 System

Song,

Luo,

Fan

et al. 2024

J. Agric. Food Chem.

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“…Several studies have established that the accumulation of the ␤-subunit Because of their abundance, these two groups of proteins are essentially responsible for the nutritive value of ␤-conglycinin is promoted either by excess nitrogen or by sulfur deficiency (Gayler and Sykes, 1985; Paek of foods and feeds derived from soybean. The glycinins, comprising approximately 40% of the total seed protein, et al, 1997Imsande and Schmidt, 1998;Imsande, 2003). An increase in the accumulation of ␤-conglycinin occur as hexamers with an estimated molecular mass of 320 to 375 kDa (Wolf and Briggs, 1958;Bradley et al, lowers the cysteine and methionine content of soybean seed protein and thus its nutritive quality.…”

Section: Soybean Is An Excellent Protein Sourcementioning

confidence: 99%

“…β‐Conglycinin, isolated as a trimer and having a molecular mass of 150 to 175 kDa (Thanh and Shibasaki, 1976, 1978), is composed of α′ (76 kDa), α (72 kDa), and β (53 kDa) subunits (Coates et al, 1985; Tierney et al, 1987). Comparison of sulfur amino acid contents indicates that glycinin contains substantially more cysteine and methionine than the β‐conglycinin (Table 1) Several studies have established that the accumulation of the β‐subunit of β‐conglycinin is promoted either by excess nitrogen or by sulfur deficiency (Gayler and Sykes, 1985; Paek et al, 1997, 2000; Imsande and Schmidt, 1998; Imsande, 2003). An increase in the accumulation of β‐conglycinin lowers the cysteine and methionine content of soybean seed protein and thus its nutritive quality.…”

Section: Abundant Soybean Seed Proteins and Their Sulfur Amino Acid C...mentioning

confidence: 99%

Engineering Soybean for Enhanced Sulfur Amino Acid Content

2005

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Soybean [Glycine max (L.) Merr.] is an important protein source in human diets and animal rations. Additionally, human consumption of soybean protein is reported to provide specific health benefits. Since methionine and cysteine contents limit the nutritional value of soybean, efforts involving both traditional breeding and genetic engineering have been employed in attempts to increase the presence of these essential amino acids. Traditional breeding has been primarily utilized to increase the total protein content but not to enhance the sulfur amino acid content of soybean. Although mutagenesis in conjunction with traditional breeding is a viable approach for enhancing the sulfur amino acid content of soybean, genetic engineering appears to be a more realistic approach. Introduction of methionine‐rich heterologous proteins has resulted in a modest increase of this amino acid in soybean. Either elevating the expression of endogenous methionine‐rich proteins or introducing synthetic proteins containing a high percentage of essential amino acids are other possible approaches that may increase the nutritional quality of the seed. Even though considerable progress has been made in enhancing the methionine content of soybean, several obstacles remain. A thorough understanding the sulfur assimilatory pathway in soybean is a prerequisite for improving the sulfur amino acid content. Expression of feedback‐insensitive forms of serine acetyl transferase and O‐acetylserine (thiol) lyase, two key enzymes in sulfur assimilatory pathway, could lead to an increase in the availability of sulfur amino acids. An adequate supply of sulfur amino acids in developing seeds may facilitate accumulation of sulfur‐rich proteins to a level sufficient to meet the nutritional requirement of livestock and poultry.

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“…Proteins are among the major constituents of a healthy and balanced human diet. Legumes have been serving as a human food-source for many centuries (Imsande, 2003 ). Notably, grain legumes (pulses) are very rich in protein-types and contribute to the human protein requirements as a primary and affordable source of proteins and minerals (Bohra et al, 2014 ).…”

mentioning

confidence: 99%

Book Review: Legumes under Environmental Stress: Yield, Improvement and Adaptations

Anjum

2016

Front. Plant Sci.

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Sulphur Nutrition and Legume Seed Quality

Cited by 5 publications

References 50 publications

Generation of New β-Conglycinin-Deficient Soybean Lines by Editing the lincRNA lincCG1 Using the CRISPR/Cas9 System

Generation of New β-Conglycinin-Deficient Soybean Lines by Editing the lincRNA lincCG1 Using the CRISPR/Cas9 System

Engineering Soybean for Enhanced Sulfur Amino Acid Content

Book Review: Legumes under Environmental Stress: Yield, Improvement and Adaptations

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