Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Clayton, Aled; Buschmann, Dominik; Byrd, James Brian; Carter, David R. F.; Cheng, Lesley; Compton, Carolyn C.; Daaboul, George; Devitt, Andrew; Falcón‐Pérez, Juan Manuel; Gardiner, Chris; Gustafson, Dakota; Harrison, Paul; Helmbrecht, Clemens; Hendrix, An; Hill, Andrew; Hoffman, Andrew M.; Jones, Jennifer C.; Kalluri, Raghu; Kang, Ji Yoon; Kirchner, Benedikt; Lässer, Cecilia; Lawson, Charlotte; Lenassi, Metka; Levin, Carina; Llorente, Alicia; Martens‐Uzunova, Elena S.; Möller, Andreas; Musante, Luca; Ochiya, Takahiro; Pink, Ryan C.; Tahara, Hidetoshi; Wauben, Marca H. M.; Webber, Jason P.; Welsh, Joshua; Witwer, Kenneth W.; Yin, Hang; Nieuwland, Rienk

doi:10.1080/20013078.2018.1473707

Cited by 60 publications

(51 citation statements)

References 31 publications

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“…First of all, EV isolation needs to become straightforward, rapid and cost-effective, with pre-analytical procedures that preserve EV structural and molecular integrity. Second, there is pressing need for gold standards for instruments and assays for detection of EV-associated biomarkers, as discussed in the latest ISEV workshop on the topic “EVs as disease biomarkers” ( Clayton et al, 2018 ). In ALS, few data are publicly available on biological fluids from patients.…”

Section: Extracellular Vesicles As Biomarkers In Alsmentioning

confidence: 99%

Role of Extracellular Vesicles in Amyotrophic Lateral Sclerosis

et al. 2018

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Amyotrophic Lateral Sclerosis (ALS) is the most common motor neuron disease in adults and primarily targets upper and lower motor neurons. The progression of the disease is mostly mediated by altered intercellular communication in the spinal cord between neurons and glial cells. One of the possible ways by which intercellular communication occurs is through extracellular vesicles (EVs) that are responsible for the horizontal transfer of proteins and RNAs to recipient cells. EVs are nanoparticles released by the plasma membrane and this review will describe all evidence connecting ALS, intercellular miscommunication and EVs. We mainly focus on mutant proteins causing ALS and their accumulation in EVs, along with the propensity of mutant proteins to misfold and propagate through EVs in prion-like behavior. EVs are a promising source of biomarkers and the state of the art in ALS will be discussed along with the gaps and challenges still present in this blooming field of investigation.

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Section: Extracellular Vesicles As Biomarkers In Alsmentioning

confidence: 99%

Role of Extracellular Vesicles in Amyotrophic Lateral Sclerosis

et al. 2018

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“…To which extent this procedure provides a comparable and reproducible impression of the overall sample quality, however, is questionable. At present, there is a strong tendency to improve the comparability and reproducibility in (bio) medical science, including the new field of EV research [5,8,9]. Therefore, as a first step, we compared operator image selection to images taken at predefined locations from the same TEM grids.…”

Section: Introductionmentioning

confidence: 99%

Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent

Rikkert

Nieuwland

Terstappen

et al. 2019

J of Extracellular Vesicle

176

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Transmission electron microscopy (TEM) has nanometre resolution and can be used to distinguish single extracellular vesicles (EVs) from non-EV particles. TEM images of EVs are a result of operator image selection. To which extent operator image selection reflects the overall sample quality, and to which extent the images are comparable and reproducible, is unclear. In a first attempt to improve the comparability and reproducibility of TEM to visualise EVs, we compared operator image selection to images taken at predefined locations from the same grids, using four EV TEM preparation protocols, a single EV-containing sample and a single TEM instrument. Operator image selection leads to high-quality images that are more similar between the protocols. In contrast, images taken at predefined locations reveal differences between the protocols, for example in number of EVs per image and background quality. From the evaluated protocols, for only one protocol the operator image selection is comparable to the TEM images taken at predefined locations. Taken together, operator image selection can be used to demonstrate the presence of EVs in a sample, but seem less suitable to demonstrate the quality of a sample. Because images taken at predefined locations reflect the overall quality of the EV-containing sample rather than the presence of EVs alone, this is a first step to improve the comparability and reproducibility of TEM for monitoring the quality of EV-containing samples.

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“…The past few years have focused on the exploration of shed vesicles, on account of that the vesicles not only deposit paracrine information, but also alter the recipient behaviors. Exosomes, a kind of extracellular vesicles that formed by the inward budding or shedding from parent cells and transferred various regulatory elements to the recipient cells, has recently captured attention in cellular communication study (6). Exosomes are nano-sized vesicles with the diameter of 20-200 nm, and carried amount of cellular components, such as RNA, DNA, protein and lipids (7).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Targeting exosomal miR-488 inhibits head and neck squamous cell carcinoma growth by mediating RAB25

Wang

Zhu

Zhao

2020

Preprint

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Background: Cancer cell-derived exosomes and its packaged miRNAs have been identified to regulate tumor growth and progression. However, its role in head and neck squamous cell carcinoma (HNSCC) and the potential mechanism still need to be further investigated. Methods: RNA sequencing was conducted to select the dysregulated genes in HNSCC. Gene ontology (GO) analysis and TCGA database were performed to analyze the potential candidate genes for HNSCC progression. Cell viability was analyzed using MTT, and colony formation was visualized using crystal violet staining. Luciferase reporter assay was employed to identify the interaction between miR-488 and RAB25. The role of miR-488 and RAB25 in tumor growth and drug response were investigated in vivo and in vitro. Results: The dysregulated genes in HNSCC captured the signaling of exosomes biogenesis dysfunction. Compared with the normal cells NP69, HNSCC cells had enriched exosomes and its packaged miRNAs, including miR-488. Luciferase reporter assay showed that RAB25 is a downstream target of miR-488. RAB25 was downregulated in HNSCC patients and predicted a poor prognosis. MiR-488/RAB25 signaling controlled cancer cell viability and colony formation ability in vitro and growth in vivo. Importantly, targeting miR-488 significantly inhibited tumor growth and promoted drug response to chemotherapy, suggesting a potential therapeutic promising for HNSCC. Conclusion These findings demonstrate a tumor-cell derived exosomal miR-488 promotes tumor growth by targeting RAB25 that could be targeted for HNSCC treatment.

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Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Cited by 60 publications

References 31 publications

Role of Extracellular Vesicles in Amyotrophic Lateral Sclerosis

Role of Extracellular Vesicles in Amyotrophic Lateral Sclerosis

Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent

WITHDRAWN: Targeting exosomal miR-488 inhibits head and neck squamous cell carcinoma growth by mediating RAB25

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