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Glass, W.A.; Varma, M.N.

doi:10.1007/978-1-4684-7627-9_19

Cited by 5 publications

(5 citation statements)

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“…This is supported by observations that damage at telomeres recruits additional response and repair factors compared to non-telomeric mitotic damage [24]. Along the same vein, cells exposed to as much as 12Gy – a dose of ionizing radiation that produces as many as ~420 DSBs per cell [27] but not specifically at the telomeres – are not the subject of an appreciable mitotic delay [6]. Thus, in the case of DNA damage-induced mitotic delay, location might be ascendant to the number of DSBs.…”

Section: Consequences Of Dna Damage During Mitosismentioning

confidence: 78%

Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

et al. 2017

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DNA double-strand breaks (DSBs) prevents cells from entering mitosis in the presence of genomic damage. Little is known about the response to DSBs once cells have already committed to mitosis. Here we review the genome-protective role of mitotic DNA damage response and evidence suggesting that its untimely activation induces chromosome segregation errors and paradoxically undermines genomic integrity. In contrast to normal cells, cancer cells coopt this pathway to propagate structural and numerical chromosomal instabilities. Cells derived from genomically unstable tumors exhibit evidence for a partially activated DNA damage response during mitosis, which leads to ongoing chromosome segregation errors. Thus, a thorough understanding of the consequences of mitotic DNA is key to our ability to devise novel anti-cancer therapeutic strategies.

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Section: Consequences Of Dna Damage During Mitosismentioning

confidence: 78%

Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

et al. 2017

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“…Oxidation in biological systems can damage biomolecules including nucleic acid and protein. Hydrogen peroxide (H 2 O 2 ) generating highly reactive radicals has been proven to play a role in the oxidation reactions . Significant quantities of H 2 O 2 can be detected as a byproduct of several metabolic pathways in human blood .…”

Section: Introductionmentioning

confidence: 99%

Quantum Chemistry Studies of Glycine−H₂O₂Complexes

Tian

2004

J. Phys. Chem. B

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Twelve glycine−H2O2 complexes are studied at the density three-parameter hybrid functional DFT-B3LYP/6-31++G(d,p) level regarding their geometries, energies, vibrational frequencies, and topological features of the electron density. Natural bond orbital (NBO) analysis and the Bader's theory of atoms in molecules (AIM) are employed to elucidate the interaction characteristics in the complexes. These complexes except for III-1 are doubly hydrogen bonding. The distinct cooperative effect and the typical resonance-assisted hydrogen-bonding mechanism are exhibited in the six-membered rings of complexes I-1 and II-1. The binding energies play a central role in the relative stabilities of the complexes, among which I-1 is the most stable conformer. A strength sequence of the hydrogen bonds from the strongest to the weakest is found: O−H···N, O−H···O, N−H···O, and C−H···O. The blue- and red-shifts in the local X−H (X = O, C, N) stretching frequencies are proportional to the bond elongations or shortening lengths. The NBO analyses show that the electronic charge is transferred from glycine to H2O2 in these complexes except for I-1 and II-1. The electron density ρ at the hydrogen bond critical point predicted by AIM is strongly correlated with the hydrogen bond parameter δRH···Y (the difference between the sum of the van der Waals radii of H and Y atoms and the length of hydrogen bond H···Y), the Fock matrix element F ij in the NBO scheme, and the interaction energy of the complex.

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“…The formation of H-type dimers characterized by blue shifts in absorption spectra and fluorescence quenching , has been the subject of a multitude of studies in a variety of physical settings. Spectral characteristics of fluorophore-containing systems ranging from simple aqueous solutions − to more complex lamellar ensembles, multilayers adsorbed onto fused quartz plates, and mixed jets in stopped flow devices have been described by exciton theory.…”

Section: Introductionmentioning

confidence: 99%

Structural Characteristics of Fluorophores That Form Intramolecular H-Type Dimers in a Protease Substrate

Packard¹,

Komoriya²,

Toptygin³

et al. 1997

J. Phys. Chem. B

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Recently, we designed and synthesized a new class of profluorescent protease substrates whose spectral properties fit the exciton model; more specifically, spectra of these polypeptides which were doubly labeled with rhodamines showed blue-shifted absorption peaks and fluorescence quenching, both indicators of H-dimer formation. In the work described here NorFES, an undecapeptide which is cleaved by the serine protease elastase, was homodoubly labeled on opposite sides of its cleavage site with six fluorophores in order to identify structural elements of dyes which influence intramolecular H-type dimer formation. Absorption and fluorescence spectra of these six substrates obtained before and after enzymatic cleavage indicate that the exciton band is strongest in the peptide doubly labeled with tetramethylrhodamine, followed by rhodamine-X, and then (diethylamino)coumarin. In contrast, spectra of NorFES homodoubly labeled with fluorescein, hydroxycoumarin, or pyrene do not exhibit exciton bands. These data suggest that factors significant in H-type dimerization are as follows (in decreasing order): delocalized charge, symmetry, and magnitude of the lowest energy electronic transition dipole. Surprisingly, in the group of fluorophores in this study, no evidence for hydrophobic interactions as an important influence was observed.

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Cited by 5 publications

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Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

Quantum Chemistry Studies of Glycine−H₂O₂Complexes

Structural Characteristics of Fluorophores That Form Intramolecular H-Type Dimers in a Protease Substrate

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Summary

Cited by 5 publications

References 0 publications

Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

Mitotic DNA Damage Response: At the Crossroads of Structural and Numerical Cancer Chromosome Instabilities

Quantum Chemistry Studies of Glycine−H2O2Complexes

Structural Characteristics of Fluorophores That Form Intramolecular H-Type Dimers in a Protease Substrate

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Product

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Quantum Chemistry Studies of Glycine−H₂O₂Complexes