Silvopasture, a traditional agroforestry practice, combines the presence of trees, shrubs, herbage, and livestock in time and space to provide multiple ecosystem services that contribute to human well-being. However, the abandonment of traditional agroforestry practices across Europe has led to substantial changes in vegetation characteristics, mainly due to woody plant expansion and, as a consequence, changes in wildlife that rely on open habitats. This study examines the effects of a 20-year abandonment of silvopastoral practices (i.e., livestock grazing and fuelwood harvesting) in a typical agroforestry Mediterranean landscape (kermes oak shrubland, natural grassland, and olive groves) on European hare (Lepus europaeus) habitat use. We estimated tree, shrub, and herb cover using a densitometer and hare habitat use using pellet counts within 2004-m2 rectangular plots in 2002, 2011, and 2021. Hare pellet density in olive groves was significantly lower in 2021 compared to 2002, while the opposite trend was found in grassland for the same period. Woody plant cover expanded from 2002 to 2021. We suggest that the woody plant encroachment that followed the abandonment of traditional silvopastoral practices in the area is the main driver behind the reported decline in hare use of the habitat, as it became less open and therefore less favorable for the species. Maintaining a mosaic of open and closed habitats at the landscape level, which was once provided by silvopastures, is vital for the conservation of this species.