Summing up particular features of protein secretion in Streptomyces lividans

Abstract

In recent years much attention has been given to the identification and characterisation of the key elements of the secretory machinery of Streptomyces lividans, a non-pathogenic filamentous Gram-positive soil bacterium, whose metabolism is relatively well characterised and capable of secreting large amounts of proteins when grown in laboratory conditions. The relevance of S. lividans from a commercial standpoint is due to its potential usefulness for the overproduction of secretory homologous and heterologous proteins of interest. Therefore, this review focuses on the knowledge already obtained on the S. lividans secretion pathways.

Co-culture of the fungusFusarium tricinctumwithStreptomyces lividansinduces production of cryptic naphthoquinone dimers

“…The EtOAc extracts of both axenic fungal cultures and cocultures resulting from mixed fermentation of F. tricinctum and S. lividans on solid rice medium were analyzed by HPLC. When fermented axenically on solid rice medium containing liquid YM medium, the fungus accumulates a complex pattern of bioactive metabolites consisting of several enniatines (11)(12)(13), polyketides (e.g. lateropyrone, 6) and lipopeptides (e.g.…”

“…The observed upregulation of antibiotically active lateropyrone (6) and enniatins (11)(12)(13) during co-cultivation of F. tricinctum and S. lividans (Table 1) may be interpreted as chemical defense of the fungus. 5 The most interesting nding from this study is, however, the observation that F. tricinctum responds to the presence of S. lividans in a metabolically different way compared to co-cultures of this fungus with B. subtilis, leading to the accumulation of the new compounds 1-4 that are not detected when the fungus is co-cultured with B. subtilis.…”

“…S. lividans is known both as a plant-endophyte 11 or as a soil-bacterium. 12,13 Co-cultivation of F. tricinctum and S. lividans resulted in the accumulation of several new compounds, the dimeric naphthoquinones (1)(2)(3)(4) and dihydrolateropyrone (5), that were not detected in axenic fungal controls (Fig. 1).…”

“…1). In addition, several known metabolites such as enniatin derivatives (11)(12)(13) showed an enhanced accumulation in the co-cultures. Structure elucidation of the new compounds by one-and two-dimensional NMR, HRMS, ECD and quantum chemical calculations is discussed.…”

Exploring the Feasibility of the Sec Route to Secrete Proteins Using the Tat Route in Streptomyces lividans

“…The second pathway, the Tat pathway, was initially discovered in the thylakoid membranes of plant plastids, and is shown to be a minor pathway responsible for exporting relatively few Tat proteins in Escherichia coli and Bacillus subtilis [3,4] in a folded conformation. Up to 27 proteins have been found to be substrates of the S. coelicolor Tat system [5,6]. TatA, TatB and TatC constitute the S. lividans Tat system [7].…”