SUMOylation and de‐SUMOylation in response to DNA damage

Dou, Hong-Liang; Huang, Chao; Nguyen, Thang Van; Long, Lu; Yeh, Edward T.H.

doi:10.1016/j.febslet.2011.04.002

Cited by 76 publications

(64 citation statements)

References 123 publications

(162 reference statements)

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“…In support, Ishida et al (2009) previously connected SUMOylation to the cell cycle based on the misregulated endocycles and high ploidy levels seen in Arabidopsis mms21 null roots. Alternatively, this endosperm-specific response could reflect the known connections of SUMO to (1) nutrient/carbohydrate metabolism, (2) epigenetic regulation, (3) responses to hypoxia-induced stress related to high metabolic activity within the endosperm, and (4) DNA damage responses common to highly proliferating tissues such as endosperm (Miura et al, 2005;Rolletschek et al, 2005;Agbor and Taylor, 2008;Sabelli and Larkins, 2009;Dou et al, 2011;Park et al, 2011Park et al, , 2012Cubeñas-Potts and Matunis, 2013).…”

Section: Discussionmentioning

confidence: 99%

Defining the SUMO System in Maize: SUMOylation Is Up-Regulated during Endosperm Development and Rapidly Induced by Stress

et al. 2016

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In response to abiotic and biotic challenges, plants rapidly attach small ubiquitin-related modifier (SUMO) to a large collection of nuclear proteins, with studies in Arabidopsis (Arabidopsis thaliana) linking SUMOylation to stress tolerance via its modification of factors involved in chromatin and RNA dynamics. Despite this importance, little is known about SUMOylation in crop species. Here, we describe the plant SUMO system at the phylogenetic, biochemical, and transcriptional levels with a focus on maize (Zea mays). In addition to canonical SUMOs, land plants encode a loosely constrained noncanonical isoform and a variant containing a long extension upstream of the signature b-grasp fold, with cereals also expressing a novel diSUMO polypeptide bearing two SUMO b-grasp domains in tandem. Maize and other cereals also synthesize a unique SUMO-conjugating enzyme variant with more restricted expression patterns that is enzymatically active despite a distinct electrostatic surface. Maize SUMOylation primarily impacts nuclear substrates, is strongly induced by high temperatures, and displays a memory that suppresses subsequent conjugation. Both in-depth transcript and conjugate profiles in various maize organs point to tissue/cell-specific functions for SUMOylation, with potentially significant roles during embryo and endosperm maturation. Collectively, these studies define the organization of the maize SUMO system and imply important functions during seed development and stress defense.

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Section: Discussionmentioning

confidence: 99%

Defining the SUMO System in Maize: SUMOylation Is Up-Regulated during Endosperm Development and Rapidly Induced by Stress

et al. 2016

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“…Interestingly, down-regulation of several subunits of the Nup107/Nup160 complex, the vertebrate ortholog of the yeast Nup84 complex, generates the accumulation of spontaneous DNA damage in human cells (Paulsen et al 2009). Mechanistically, concentration of the SUMO machinery at the NPC could modulate proper ubiquitination levels of intervening factors and facilitate repair (Palancade et al 2007;Nagai et al 2008;Dou et al 2011). Thus, the yeast NPC can also accommodate a favorable environment for DNA repair, preventing genome instability.…”

Section: Dna Replication and Genome Integrity Maintenancementioning

confidence: 99%

Nuclear pore proteins and the control of genome functions

2015

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Nuclear pore complexes (NPCs) are composed of several copies of ∼30 different proteins called nucleoporins (Nups). NPCs penetrate the nuclear envelope (NE) and regulate the nucleocytoplasmic trafficking of macromolecules. Beyond this vital role, NPC components influence genome functions in a transport-independent manner. Nups play an evolutionarily conserved role in gene expression regulation that, in metazoans, extends into the nuclear interior. Additionally, in proliferative cells, Nups play a crucial role in genome integrity maintenance and mitotic progression. Here we discuss genome-related functions of Nups and their impact on essential DNA metabolism processes such as transcription, chromosome duplication, and segregation.

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“…The SUMO ligase activity of MMS21 contributes to the roles of the SMC5/6 complex in recombinational repair, collapsed replication fork restart, and ribosomal DNA and telomere maintenance, with some relevant substrates having been identified (Potts and Yu, 2005;Zhao and Blobel, 2005). Although protein sumoylation plays important roles in the maintenance of genomic stability from yeast (Saccharomyces cerevisiae) to humans (Dou et al, 2011), it is unknown whether plants possess sumoylation mechanisms that act in the response to DNA damage. In Arabidopsis, AtMMS21/HIGH PLOIDY2 (HPY2) is a functional SUMO ligase and plays an important role in plant root development (Huang et al, 2009;Ishida et al, 2009), but the precise mechanism of AtMMS21 in regulating root meristem function is unclear.…”

mentioning

confidence: 99%

“…SUMO (for small ubiquitin-related modifier) has emerged as a significant regulator of genomic stability (Dou et al, 2011). MMS21 (for methyl methanesulfonate sensitivity gene21) encodes a SUMO E3 ligase that forms a critical component of the STRUCTURAL MAINTENANCE OF CHROMOSOMES5/6 (SMC5/6) complex, which is an evolutionarily conserved chromosomal ATPase required for cell growth and DNA repair (Duan et al, 2009).…”

mentioning

confidence: 99%

AtMMS21, an SMC5/6 Complex Subunit, Is Involved in Stem Cell Niche Maintenance and DNA Damage Responses in Arabidopsis Roots

Yuan

Liu

et al. 2013

Plant Physiology

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Plants maintain stem cells in meristems to sustain lifelong growth; these stem cells must have effective DNA damage responses to prevent mutations that can propagate to large parts of the plant. However, the molecular links between stem cell functions and DNA damage responses remain largely unexplored. Here, we report that the small ubiquitin-related modifier E3 ligase AtMMS21 (for methyl methanesulfonate sensitivity gene21) acts to maintain the root stem cell niche by mediating DNA damage responses in Arabidopsis (Arabidopsis thaliana). Mutation of AtMMS21 causes defects in the root stem cell niche during embryogenesis and postembryonic stages. AtMMS21 is essential for the proper expression of stem cell niche-defining transcription factors. Moreover, mms21-1 mutants are hypersensitive to DNA-damaging agents, have a constitutively increased DNA damage response, and have more DNA double-strand breaks (DSBs) in the roots. Also, mms21-1 mutants exhibit spontaneous cell death within the root stem cell niche, and treatment with DSB-inducing agents increases this cell death, suggesting that AtMMS21 is required to prevent DSB-induced stem cell death. We further show that AtMMS21 functions as a subunit of the STRUCTURAL MAINTENANCE OF CHROMOSOMES5/6 complex, an evolutionarily conserved chromosomal ATPase required for DNA repair. These data reveal that AtMMS21 acts in DSB amelioration and stem cell niche maintenance during Arabidopsis root development.

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SUMOylation and de‐SUMOylation in response to DNA damage

Cited by 76 publications

References 123 publications

Defining the SUMO System in Maize: SUMOylation Is Up-Regulated during Endosperm Development and Rapidly Induced by Stress

Defining the SUMO System in Maize: SUMOylation Is Up-Regulated during Endosperm Development and Rapidly Induced by Stress

Nuclear pore proteins and the control of genome functions

AtMMS21, an SMC5/6 Complex Subunit, Is Involved in Stem Cell Niche Maintenance and DNA Damage Responses in Arabidopsis Roots

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