2015
DOI: 10.1093/nar/gkv842
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Sumoylation of Sir2 differentially regulates transcriptional silencing in yeast

Abstract: Silent information regulator 2 (Sir2), the founding member of the conserved sirtuin family of NAD+-dependent histone deacetylase, regulates several physiological processes including genome stability, gene silencing, metabolism and life span in yeast. Within the nucleus, Sir2 is associated with telomere clusters in the nuclear periphery and rDNA in the nucleolus and regulates gene silencing at these genomic sites. How distribution of Sir2 between telomere and rDNA is regulated is not known. Here we show that Si… Show more

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Cited by 19 publications
(18 citation statements)
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“…Similar to S. cerevisiae (48,49), SUMOylation appears to be involved in the regulation of the telomere position effect in C. glabrata. Adherence to the host tissue, an important virulence attribute of C. glabrata, is mediated by a large family of cell wall proteins, including Epa adhesins (30,40).…”
Section: Discussionmentioning
confidence: 87%
See 1 more Smart Citation
“…Similar to S. cerevisiae (48,49), SUMOylation appears to be involved in the regulation of the telomere position effect in C. glabrata. Adherence to the host tissue, an important virulence attribute of C. glabrata, is mediated by a large family of cell wall proteins, including Epa adhesins (30,40).…”
Section: Discussionmentioning
confidence: 87%
“…Western Blot-Whole cell extracts were made from overnight C. glabrata cultures using trichloroacetic acid (TCA) precipitation (49). An equal number of cells as measured by absorbance were used for protein extractions, and protein levels in samples were estimated by SDS-PAGE/Ponceau-stained blots and, in some cases, additionally by Pierce BCA kit.…”
Section: Methodsmentioning
confidence: 99%
“…Indeed, Sir2 was recently reported to be modified and regulated by sumoylation and phosphorylation (Hannan et al 2015;Kang et al 2015). Sir2 is sumoylated by the SUMO ligase Siz2 on three lysines (K106, K132, and K215) within the N-terminal extension domain that associates with Sir4.…”
Section: Sir2 Post-translational Modificationsmentioning
confidence: 99%
“…Sir2 is sumoylated by the SUMO ligase Siz2 on three lysines (K106, K132, and K215) within the N-terminal extension domain that associates with Sir4. Modification of K215 appears to be important in regulating Sir2 distribution between telomeres and the rDNA (Hannan et al 2015), perhaps by disrupting the Sir2-Sir4 interaction.…”
Section: Sir2 Post-translational Modificationsmentioning
confidence: 99%
“…40 Sumoylation of the Sir2 histone deacetylase disrupts its interaction with the telomeric silencing complex, further promoting transcriptional derepression at subtelomeres. 41 Sumoylation of the Hpr1 subunit of the THO complex regulates mRNP assembly, 28 possibly impacting on transcriptional elongation. Epistasis analyses further support the fact that some of the contributions of Ulp1 to gene expression depend on Hpr1 sumoylation.…”
Section: A Role For the Nup84 Complex And Ulp1 In Gene Transcriptionmentioning
confidence: 99%