Sumoylation of the Plant Clock Transcription Factor CCA1 Suppresses DNA Binding

Hansen, Louise L.; Imrie, Lisa; Bihan, Thierry Le; Burg, H.A. van den; Ooijen, Gerben van

doi:10.1177/0748730417737695

Cited by 15 publications

(8 citation statements)

References 43 publications

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“…Mutation of SPF1 and SPF2 genes also results in the misexpression of generative and embryo-specific genes. SPF1 has been reported to interact with Embryo sac Development Arrest 19 (EDA19) [ 62 , 80 , 81 ].…”

Section: Sumo Conjugation and Deconjugation In The Nucleusmentioning

confidence: 99%

An Insight into the Factors Influencing Specificity of the SUMO System in Plants

Srivastava

Sadanandom

2020

Plants

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Due to their sessile nature, plants are constantly subjected to various environmental stresses such as drought, salinity, and pathogen infections. Post-translational modifications (PTMs), like SUMOylation, play a vital role in the regulation of plant responses to their environment. The process of SUMOylation typically involves an enzymatic cascade containing the activation, (E1), conjugation (E2), and ligation (E3) of SUMO to a target protein. Additionally, it also requires a class of SUMO proteases that generate mature SUMO from its precursor and cleave it off the target protein, a process termed deSUMOylation. It is now clear that SUMOylation in plants is key to a plethora of adaptive responses. How this is achieved with an extremely limited set of machinery components is still unclear. One possibility is that novel SUMO components are yet to be discovered. However, current knowledge indicates that only a small set of enzymes seem to be responsible for the modification of a large number of SUMO substrates. It is yet unknown where the specificity lies within the SUMO system. Although this seems to be a crucial question in the field of SUMOylation studies, not much is known about the factors that provide specificity. In this review, we highlight the role of the localisation of SUMO components as an important factor that can play a vital role in contributing to the specificity within the process. This will introduce a new facet to our understanding of the mechanisms underlying such a dynamic process.

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Section: Sumo Conjugation and Deconjugation In The Nucleusmentioning

confidence: 99%

An Insight into the Factors Influencing Specificity of the SUMO System in Plants

Srivastava

Sadanandom

2020

Plants

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“…Post-translational modification also plays a vital role in ensuring the robustness of the core oscillator. For instance, many components of the core oscillator, including CCA1 and PRRs, are subjected to post-translational modifications such as phosphorylation (Sugano et al, 1998;Daniel et al, 2004;Fujiwara et al, 2008;Wang et al, 2010;Uehara et al, 2019), sumoylation (Hansen et al, 2017), and ubiquitination (Han et al, 2004;Baudry et al, 2010). Post-translational modifications are critical for maintaining protein activities and stability and subsequently play fundamental roles in an intricate control of the circadian clock.…”

Section: Introductionmentioning

confidence: 99%

Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock

Wang

et al. 2020

Molecular Plant

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Post-translational modifications play essential roles in finely modulating eukaryotic circadian clock systems. In plants, the effects of O-glycosylation on the circadian clock and the underlying mechanisms remain largely unknown. The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transferase SECRET AGENT (SEC) are two prominent O-glycosylation enzymes in higher plants, with both overlapped and unique functions in plant growth and development. Unlike the critical role of O-GlcNAc in regulating the animal circadian clock, here we report that nuclearlocalized SPY, but not SEC, specifically modulates the pace of the Arabidopsis circadian clock. By identifying the interactome of SPY, we identified PSEUDO-RESPONSE REGULATOR 5 (PRR5), one of the core circadian clock components, as a new SPY-interacting protein. PRR5 can be Ofucosylated by SPY in planta, while point mutation in the catalytic domain of SPY abolishes the O-fucosylation of PRR5. The protein abundance of PRR5 is strongly increased in spy mutants, while the degradation rate of PRR5 is much reduced, suggesting that PRR5 proteolysis is promoted by SPY-mediated O-fucosylation. Moreover, multiple lines of genetic evidence indicate that PRR5 is a major downstream target of SPY to specifically mediate its modulation of the circadian clock. Collectively, our findings provide novel insights into the specific role of the Ofucosyltransferase activity of SPY in modulating the circadian clock and implicate that Oglycosylation might play an evolutionarily conserved role in modulating the circadian clock system, via O-GlcNAcylation in mammals, but via O-fucosylation in higher plants.

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“…Neither the localization nor the stability of CCA1 is significantly affected by its SUMOylation state. However binding of CCA1 to the evening element within the PRR9 promoter was significantly reduced in the ots1 ots2 mutant, indicating SUMOylation suppresses the binding activity of CCA1 to target genes [97].…”

Section: Sumoylationmentioning

confidence: 96%

“…Analyses of circadian rhythm in ots1 ots2 and siz1 mutants at different temperatures showed that the clock in siz1 is undercompensated at higher temperatures, whereas the clock in ots1 ots2 is undercompensated at the lower temperatures, suggesting the level of SUMOylation contributes to temperature compensation of clock [96]. Subsequent work showed CCA1 SUMOylation in vivo oscillates in similar proportion to the abundance of the protein [97]. Neither the localization nor the stability of CCA1 is significantly affected by its SUMOylation state.…”

Section: Sumoylationmentioning

confidence: 98%

Post-Translational Mechanisms of Plant Circadian Regulation

Yan

Kim

Somers

2021

Genes

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The molecular components of the circadian system possess the interesting feature of acting together to create a self-sustaining oscillator, while at the same time acting individually, and in complexes, to confer phase-specific circadian control over a wide range of physiological and developmental outputs. This means that many circadian oscillator proteins are simultaneously also part of the circadian output pathway. Most studies have focused on transcriptional control of circadian rhythms, but work in plants and metazoans has shown the importance of post-transcriptional and post-translational processes within the circadian system. Here we highlight recent work describing post-translational mechanisms that impact both the function of the oscillator and the clock-controlled outputs.

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Sumoylation of the Plant Clock Transcription Factor CCA1 Suppresses DNA Binding

Cited by 15 publications

References 43 publications

An Insight into the Factors Influencing Specificity of the SUMO System in Plants

An Insight into the Factors Influencing Specificity of the SUMO System in Plants

Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock

Post-Translational Mechanisms of Plant Circadian Regulation

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