Sunflower

Paniego, Norma; Heinz, Ruth Amelia; Fernández, Paula; Talia, Paola; Nishinakamasu, Verónica; Hopp, H. Esteban

doi:10.1007/978-3-540-34388-2_4

Cited by 18 publications

(10 citation statements)

References 134 publications

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“…BAC-FISH techniques have not been widely used in sunflower mainly due to difficulties in chromosome preparations (Paniego et al 2007). Signal sensitivity of in situ hybridization largely relies on the use of high-quality metaphase preparations with well-spread chromosomes that are free from cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…In the past decades, various genetic markers and the corresponding linkage maps have been developed, such as restriction fragment length polymorphism [RFLP (Berry et al 1995, 1997; Gentzbittel et al 1995, 1999; Jan et al 1998)], simple sequence repeats [SSR (Gedil et al 2001; Paniego et al 2002; Tang et al 2002, 2003; Yu et al 2002, 2003)], expressed sequence tag (EST)-SSR, EST-INDEL (insertion or deletion), and EST−single-nucleotide polymorphism (Lai et al 2005; Pashley et al 2006; Heesacker et al 2008)]. Moreover, the consensus genetic maps integrating the independently developed linkage maps based on the shared markers have been reported (Yu et al 2003; Paniego et al 2007). Yu et al (2003) integrated 120 SSR loci from the public SSR map and 28 RFLP markers from Jan et al (1998) map to a backbone of 80 RFLP loci on the genetic map of Berry et al (1997).…”

mentioning

confidence: 99%

“…Yu et al (2003) integrated 120 SSR loci from the public SSR map and 28 RFLP markers from Jan et al (1998) map to a backbone of 80 RFLP loci on the genetic map of Berry et al (1997). Another high-density composite genetic map (Paniego et al 2007) was established by integrating a set of 161 SSR marker loci (Al-Chaarani et al 2004) and 58 HA-SSR markers (Paniego et al 2002) to the public SSR map (Tang et al 2003). These maps with almost full sunflower genome coverage allow for the cross-reference with each other and provide a dense genome-wide framework for sunflower research.…”

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confidence: 99%

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Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

Feng

Liu

Cai

et al. 2013

G3 Genes|Genomes|Genetics

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Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivated sunflower cv. HA89. In the present study, we used these linkage group-specific clones (∼100 kb in size) as probes to in situ hybridize to HA89 mitotic chromosomes at metaphase using the BAC- fluorescence in situ hybridization (FISH) technique. Because a characteristic of the sunflower genome is the abundance of repetitive DNA sequences, a high ratio of blocking DNA to probe DNA was applied to hybridization reactions to minimize the background noise. As a result, all sunflower chromosomes were anchored by one or two BAC/BIBAC clones with specific FISH signals. FISH analysis based on tandem repetitive sequences, such as rRNA genes, has been previously reported; however, the BAC-FISH technique developed here using restriction fragment length polymorphism (RFLP)−derived BAC/BIBAC clones as probes to apply genome-wide analysis is new for sunflower. As chromosome-specific cytogenetic markers, the selected BAC/BIBAC clones that encompass the 17 linkage groups provide a valuable tool for identifying sunflower cytogenetic stocks (such as trisomics) and tracking alien chromosomes in interspecific crosses. This work also demonstrates the potential of using a large-insert DNA library for the development of molecular cytogenetic resources.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

Feng

Liu

Cai

et al. 2013

G3 Genes|Genomes|Genetics

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“…Sunflower (Helianthus annuus L.) is one of the major sources for edible oil production worldwide, ranking fourth after soybean, palm and rapeseed (canola), accounting for approximately 13% of the world's total edible oil production (Paniego et al 2007). Sunflower seed is consumed as confectionary product as well.…”

Section: Introductionmentioning

confidence: 99%

Morphology, phylogeny and pathogenicity ofAlternariaspecies, involved in leaf spot disease of sunflower in northern Iran

Khodaei

Arzanlou

2013

Archives Of Phytopathology And Plant Protection

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Leaf spot disease of sunflower is one of the most important foliar diseases on this crop worldwide. Several fungal groups are known to cause leaf spot disease on sunflower. Species of the genus Alternaria are the most common and serious leaf spot causing fungi on this crop. Leaf spot disease is the most destructive foliar diseases on sunflower in northern Iran; however, the identity of the causal agent remains unknown. The present study was aimed to characterise the identity of the causal agent of the disease by means of morphological and molecular data as well as to evaluate the pathogenicity of the responsible species. For this purpose, a total number of 97 fungal isolates were recovered from sunflower leaves with leaf spot disease symptoms from the sunflower fields in northwestern zone of Iran. All of the isolates were identified as Alternaria alternata based on cultural and morphological characteristics. A subset of isolates was subjected to phylogenetic analysis using sequence data from ITS-rDNA region, gpd and rpb2 genes. Sequence data from ITS-rDNA and gpd did not discriminate A. alternata from the other small-spored Alternaria species. A phylogeny inferred using sequence data from rpb2 gene clustered our isolates in several sub-clades within a single monophyletic clade. Sequence data for the type strain of the other small-spored Alternaria species has to be included in phylogenetic analysis, in order to make sure, whether the observed variations in rpb2 gene sequences are an indication for the population variation in sunflower isolates of A. alternata or define species boundaries among the small-spored Alternaria species. The results of pathogenicity assay on sunflower plants (cultivar Euroflor) under greenhouse condition revealed that A. alternata is pathogenic on sunflower.

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“…Such differences could be increased because of the inability of current hybrids to retain its green leaf area for a longer period, especially during the grain filling phase; therefore, senescence onset and rate are key determinants of yield and oil content in sunflower [29,30]. [18,21,[31][32][33][34][35][36][37][38][39][40][41]. Recently, a characterization of leaf senescence process in sunflower was developed through a systems biology approach integrating transcriptomic and metabolomic analyses, thus allowing the detection of early metabolic changes prior to anthesis and before the onset of the first senescence symptoms [42].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)

et al. 2016

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Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.

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Sunflower

Cited by 18 publications

References 134 publications

Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

Morphology, phylogeny and pathogenicity ofAlternariaspecies, involved in leaf spot disease of sunflower in northern Iran

Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)

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