Sunlight Brightens Learning and Memory

Chantranupong, Lynne; Sabatini, Bernardo L.

doi:10.1016/j.cell.2018.05.044

Cited by 9 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[40] It has been reported that exposure to light improves brain responses to cognitive tasks, and short-and long-term plasticity. [41,42] To mimic the memory consolidation effect of biological synapse, we illuminated 1 mW cm -2 of polychromatic light on the DGOST-DREGE device (Figure S11, Supporting Information). Light-induced postsynaptic signal enhancement and charge retention were clearly observed from the DGOST-DREGE device, indicating that our device behaves similarly to an actual synapse.…”

Section: Resultsmentioning

confidence: 99%

A Hippocampus‐Inspired Dual‐Gated Organic Artificial Synapse for Simultaneous Sensing of a Neurotransmitter and Light

Lee

2021

Advanced Materials

View full text Add to dashboard Cite

such as light or pressure as inputs. [7,[9][10][11][12][13] Little effort has been made to develop organic artificial synapses that can detect chemicals, especially analytes in a liquid medium.Among the platforms of organic synapses, electrolyte-based organic synaptic transistors have been widely studied because of their high biocompatibility required for future applications of artificial sensory synaptic system from in vitro to in vivo. [14][15][16][17] In order to optimize the synaptic performance and expand the functionality of electrolyte-based synaptic transistors, state-of-the-art organic artificial synapse studies are attempting to implement two or more types of synapse-driving mechanisms in a single device or devise new synaptic device structure. For example, a ferroelectric/electrochemical synapse successfully supplemented conventional electrochemical transistors with longer persistent nonvolatile plasticity and unique threshold switching properties. [18] Also, parallel programming of organic synapses has been demonstrated by connecting a gate terminal of an organic redox transistor to a diffusive memristor. [19] Therefore, in order to expand functionality of various organic synapses, it is necessary to explore organic synaptic transistors with various operation mechanisms and device structures and, as a result, combine their advantageous characteristics.In a biological synapse, which is a principal emulation target of neuromorphic devices, excitation or inhibition occurs via the release or reception of neurotransmitters. Therefore, it is of great benefit to develop artificial sensory synaptic system which recognizes neurotransmitter signals and modulates postsynaptic currents including potentiation/depression, paired-pulse facilitation, and Hebbian learning. [20][21][22] Among various neurotransmitters, dopamine plays an important role in learning and memory consolidating functions of hippocampal memory, affecting plasticity, synaptic transmission, and network activity in the hippocampal circuitry. [23][24][25] Recently, a biohybrid synapse with dopaminergic PC-12 cells coupled to an organic neuromorphic device has been reported to enable dopamine signaling in artificial synapses. [26] This approach was based on continuous exposure of both sensing and signal processing parts to the wet environment, cell incubation, and immobilizing process. Thus, it will also be interesting to develop a dopaminergic signaling Organic neuromorphic devices and sensors that mimic the functions of chemical synapses and sensory perception in humans have received much attention for next-generation computing and integrated logic circuits. Despite recent advances, organic artificial synapses capable of detecting both neurotransmitters in liquid environments and light are not reported. Herein, inspired by hippocampal synapses, a dual-gate organic synaptic transistor platform with a photoconductive polymer semiconductor, a ferroelectric insulator of P(VDF-TrFE), and an extended-gate electrode functionalized with boronic acid is d...

show abstract

Section: Resultsmentioning

confidence: 99%

A Hippocampus‐Inspired Dual‐Gated Organic Artificial Synapse for Simultaneous Sensing of a Neurotransmitter and Light

Lee

2021

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…In PD, an in vivo study has shown that chronic unregulated cytosolic dopamine alone is enough to cause neurodegeneration [ 196 ]. Glutamate is an excitatory neurotransmitter implicated in the regulation of neurogenesis, synaptogenesis, memory, and neuronal plasticity [ 197 , 198 , 199 , 200 ]. This is especially true of the action of glutamate at synaptic NMDARs; however, glutamate activity at extrasynaptic NMDARs can lead to neurotoxicity and cell death [ 201 , 202 , 203 ].…”

Section: Leveraging Gefb and Ipsc Technologies For Pre-clinical Appli...mentioning

confidence: 99%

Seeing Neurodegeneration in a New Light Using Genetically Encoded Fluorescent Biosensors and iPSCs

Stellon

Talbot

Hewitt

et al. 2023

IJMS

View full text Add to dashboard Cite

Neurodegenerative diseases present a progressive loss of neuronal structure and function, leading to cell death and irrecoverable brain atrophy. Most have disease-modifying therapies, in part because the mechanisms of neurodegeneration are yet to be defined, preventing the development of targeted therapies. To overcome this, there is a need for tools that enable a quantitative assessment of how cellular mechanisms and diverse environmental conditions contribute to disease. One such tool is genetically encodable fluorescent biosensors (GEFBs), engineered constructs encoding proteins with novel functions capable of sensing spatiotemporal changes in specific pathways, enzyme functions, or metabolite levels. GEFB technology therefore presents a plethora of unique sensing capabilities that, when coupled with induced pluripotent stem cells (iPSCs), present a powerful tool for exploring disease mechanisms and identifying novel therapeutics. In this review, we discuss different GEFBs relevant to neurodegenerative disease and how they can be used with iPSCs to illuminate unresolved questions about causes and risks for neurodegenerative disease.

show abstract

“…Glutamate is the predominant excitatory neurotransmitter in the brain ( Curtis and Johnston, 1974 ; Fonnum, 1984 ) and is critical to numerous central nervous system (CNS) functions ( Tang et al, 1999 ; Matsuzaki et al, 2004 ; Chantranupong and Sabatini, 2018 ; Boender et al, 2020 ; Stephenson-Jones et al, 2020 ). Anomalously elevated glutamate in the synapse results in CNS neurotoxicity; removal of glutamate occurs via glutamate transporters (excitatory amino acid transporters; EAATs), as enzymes that metabolize glutamate in the synaptic cleft are scarce.…”

Section: Introductionmentioning

confidence: 99%

The Conformationally Sensitive Spatial Distance Between the TM3-4 Loop and Transmembrane Segment 7 in the Glutamate Transporter Revealed by Paired-Cysteine Mutagenesis

Wang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Excitatory amino acid transporters can maintain extracellular glutamate concentrations lower than neurotoxic levels by transferring neurotransmitters from the synaptic cleft into surrounding glial cells and neurons. Previous work regarding the structural studies of GltPh, GltTK, excitatory amino acid transporter 1 (EAAT1), EAAT3 and alanine serine cysteine transporter 2 described the transport mechanism of the glutamate transporter in depth. However, much remains unknown about the role of the loop between transmembrane segment 3 and 4 during transport. To probe the function of this loop in the transport cycle, we engineered a pair of cysteine residues between the TM3-TM4 loop and TM7 in cysteine-less EAAT2. Here, we show that the oxidative cross-linking reagent CuPh inhibits transport activity of the paired mutant L149C/M414C, whereas DTT inhibits the effect of CuPh on transport activity of L149C/M414C. Additionally, we show that the effect of cross-linking in the mutant is due to the formation of the disulfide bond within the molecules of EAAT2. Further, L-glutamate or KCl protect, and D,L-threo-β-benzyloxy-aspartate (TBOA) increases, CuPh-induced inhibition in the L149C/M414 mutant, suggesting that the L149C and M414C cysteines are closer or farther away in the outward- or inward-facing conformations, respectively. Together, our findings provide evidence that the distance between TM3-TM4 loop and TM7 alter when substrates are transported.

show abstract

Sunlight Brightens Learning and Memory

Cited by 9 publications

References 10 publications

A Hippocampus‐Inspired Dual‐Gated Organic Artificial Synapse for Simultaneous Sensing of a Neurotransmitter and Light

A Hippocampus‐Inspired Dual‐Gated Organic Artificial Synapse for Simultaneous Sensing of a Neurotransmitter and Light

Seeing Neurodegeneration in a New Light Using Genetically Encoded Fluorescent Biosensors and iPSCs

The Conformationally Sensitive Spatial Distance Between the TM3-4 Loop and Transmembrane Segment 7 in the Glutamate Transporter Revealed by Paired-Cysteine Mutagenesis

Contact Info

Product

Resources

About