¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Abstract

Introduction Nicotiana tabacum is a plant model intensively used in the bio‐engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non‐targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available. Objective To compare the proton nuclear magnetic resonance (1H‐NMR) metabolomics profiling of a recombinant Nicotiana tabacum strain containing a promoter of a sesquiterpene cyclase from Capsicum annuum driving GUS expression, versus wild‐type samples. Methodology The non‐targeted 1H‐NMR metabolome was obtained and processed by principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS‐DA). The differential metabolites were quantified by quantitative NMR. Results PCA and OPLS‐DA revealed 37 metabolites including 16 discriminant compounds for transgenic samples. Ethanol (0.4 mg g‐1), the main differential compound, was exclusively detected in transgenic tobacco; however, high levels of formate (0.28 mg g‐1) and acetate (0.3 mg g‐1) were simultaneously observed in the same group of samples. Cembratriene‐4,6‐diol, an antitumour and neuroprotective compound, and capsidiol, a known phytoalexin, increased by about 30% in transgenic samples. In addition, the endogenous levels of the antioxidant caffeoylquinic acid isomers increased by 50% in comparison to those of wild‐type tobaccos. Conclusion Our results support the occurrence of metabolic differences between wild type and transgenic tobacco containing a promoter of a Capsicum sesquiterpene cyclase gene. Interestingly, the recombinant transgenic strain studied accumulated high amounts of added value compounds with biological activity.

Untargeted 1H‐NMR Metabolome of Celery During Fusarium Wilt: Implications for Vegetable Quality

“…The first one could involve the colonization of saprophytic microorganisms such as yeasts which convert assimilable sugars into methanol. [22] The second is regarded to the degradation of cell walls leading to the hydrolysis of cell polysaccharides to produce methanol. [14] Previous studies revealed that plants subjected to chronic stress such as those containing recombinant DNA, can accumulate high amounts of methanol.…”

Influence of Environmental Factors on the Genetic and Chemical Diversity of Brickellia veronicifolia Populations Growing in Fragmented Shrublands from Mexico

“…Validation was done with the R 2 x and Q 2 values using 999 permutations analysis and performed with SIMCA ver. 16.0 [77].…”

Antivirus isoindolinone alkaloids with rare oxocyclopenta[f]isoindole frameworks isolated from the stems of flue cured tobacco

“…In addition, tobacco also has important significance to humans for their ornamental values and medicinal uses [2]. In recent years, many bioactive naturally small molecule metabolites have been reported from this genus, including diterpenoids [3], sesquiterpenoids [4,5], flavonoids [6,7], coumarins [8,9], furans [10,11], alkaloids [12,13], sterols [6], and the like. The biosynthesis and pharmacological properties of some metabolites also have been widely elucidated, such as pyridine alkaloids, including the eponymous nicotine [14], isoprenoids [15], aromatic compounds [16], and polyphenols [17].…”

“…Fig 3. Plausible biosynthetic pathway of the oxocyclopentane moieties in nicoisoindoles A-D(1)(2)(3)(4) …”