¹H NMR Study on the Short- and Long-Term Impact of Two Training Programs of Sprint Running on the Metabolic Fingerprint of Human Serum

Abstract

Metabonomics is an established strategy in the exploration of the effects of various stimuli on the metabolic fingerprint of biofluids. Here, we present an application of (1)H NMR-based metabonomics on the field of exercise biochemistry. Fourteen men were assigned to either of two training programs, which lasted 8 weeks and involved sets of 80-m maximal runs separated by either 10 s or 1 min of rest. Analysis of pre- and postexercise serum samples, both at the beginning and end of training, by (1)H NMR spectroscopy and subsequent multivariate statistical techniques revealed alterations in the levels of 18 metabolites. Validated O-PLS models could classify the samples in regard to exercise, the separation being mainly due to lactate, pyruvate, alanine, leucine, valine, isoleucine, arginine/lysine, glycoprotein acetyls, and an unidentified metabolite resonating at 8.17 ppm. Samples were also classified safely with respect to training, the separation being mainly due to lactate, pyruvate, methylguanidine, citrate, glucose, valine, taurine, trimethylamine N-oxide, choline-containing compounds, histidines, acetoacetate/acetone, glycoprotein acetyls, and lipids. Samples could not be classified according to the duration of the rest interval between sprints. Our findings underline the power of metabonomics to offer new insights into the short- and long-term impact of exercise on metabolism.

Global Metabolic Stress of Isoeffort Continuous and High Intensity Interval Aerobic Exercise: A Comparative 1H NMR Metabonomic Study

“…Our results imply that when matched for overall effort and strain (RPE and HR peak ) the three endurance protocols may produce similar overall metabolic challenge and possibly similar muscular long-term adaptations [3][4][5]29 . This is in line with a recent study showing that two different sprint training programs consisting of maximal runs showed similar acute and long-term impact on serum metabolic fingerprint 14 . Thus, the overall metabolic response is mostly tied up to the overall effort and strain rather than to the characteristics (intensity, duration, work-rest transitions) of the endurance protocol per se.…”

“…Therefore, metabonomics is being increasingly used to examine the metabolic stress to a single exercise bout [7][8][9][10][11][12][13][14] .…”

Quality Markers Addressing Preanalytical Variations of Blood and Plasma Processing Identified by Broad and Targeted Metabolite Profiling

“…Examples of metabolites that are highly affected by preanalytics and previously reported as biomarkers in various biomed-ical indications (11,(33)(34)(35)(36)(37)(38)(39) are serotonin, taurine, sphingosine-1-phosphate, glutamine, and glutamate (Fig. 2C, Fig.…”

Serum Nuclear Magnetic Resonance Spectroscopy: One More Step toward Clinical Utility

“…More recently, in the NMR-based metabolomics-type of applications, increased circulating glycoproteins have been linked to rheumatoid arthritis and its severity (7 ) as well as to cardiovascular and cancer mortality (4 ). Differences in glycoprotein concentrations have also been seen during pregnancy (8 ), in relation to liver fibrosis (9 ), after exercise (10 ), and owing to long-term physical activity (11 ). Increased adiposity causally affects increased circulating glycoprotein concentrations (12 ).…”